Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2016, 4(4), 210-215
DOI: 10.12691/jfnr-4-4-3
Open AccessArticle

Reduction of Adipogenesis by Chokeberry (Aronia melanocarpa) Extract in 3T3-L1 Preadipocytes

Jung-Hee Shin1 and Ji-Hye Jung2,

1Department of food & nutrition, Joongbu University, Chungnam, Korea

2Institute for Clinical Nutrition, INJE University, Seoul, Korea

Pub. Date: May 16, 2016

Cite this paper:
Jung-Hee Shin and Ji-Hye Jung. Reduction of Adipogenesis by Chokeberry (Aronia melanocarpa) Extract in 3T3-L1 Preadipocytes. Journal of Food and Nutrition Research. 2016; 4(4):210-215. doi: 10.12691/jfnr-4-4-3


The aim of this study was investigate the anti-obesity effect and mechanism of action of chokeberry (Aronia melanocarpa) extract (CBE) in 3T3-L1 cells. CBE was tested for its antioxidant compounds content (total phenol and flavonoid). Lipid accumulation levels were measured by determining the expression of genes associated with adipocyte differentiation and adipokine secretion in 3T3-L1 cells. In CBE, the total phenolic content was 212.0 ± 3.11 mg/g and the flavonoids content was 113.25 ± 2.5 mg/g. Oil Red O staining showed that CBE inhibited lipid accumulation at concentration of 0, 0.5, and 1.0 mg/mL in a dose-dependent manner. CBE decreased the expression of the key adipocyte differentiation regulator peroxisome proliferator-activated receptor-γ and the fatty acid binding protein 4 gene during the differentiation of preadipocytes into adipocytes. Moreover, CBE down regulated adipokine-specific genes such as leptin and monocyte chemoattractant protein-1 relative to non-treated adipocytes. These results demonstrated that CBE inhibited adipogenesis by down regulating peroxisome proliferator-activated receptor-γ and fatty acid binding protein 4 and by reducing adipokine expression in 3T3-L1 cells.

chokeberry extract adipocyte differentiation adipokine secretion anti-obesity

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[1]  Ministry of Health and Welfare, “2012 Korean National Health & Nutrition Examination Survey,” [accessed Nov. 02, 2016].
[2]  Ogden, C.L., Carroll, M.D., Kit, B.K., Flegal, K.M., “Prevalence of obesity among adults: United States, 2011-2012,” NCHS, Data Brief, 131, 2013.
[3]  Yaemsiri, S., Slining, M.M., Agarwal, S.K., “Perceived weight status, overweight diagnosis, and weight control among US adults: the NHANES 2003-2008 Study,” International Journal of Obesity (Lond), 35(8), 1063-70, 2011.
[4]  Tontonoz, P., Hu, E., Spiegelman, B.M., “Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor,” Cell, 79(7), 1147-56, 1994.
[5]  Ranganathan, G., Unal, R., Pokrovskaya, I., Yao-Borengasser, A., Phanavanh, B., Lecka-Czernik, B., Rasouli, N., Kern, P.A., “The lipogenic enzymes DGAT1, FAS, and LPL in adipose tissue: effects of obesity, insulin resistance, and TZD treatment,” Journal of Lipid Research, 47(11), 2444-50, 2006.
[6]  Chang, E., Choi, J.M., Kim, W.J., Rhee, E.J., Oh, K.W., Lee, W.Y., Park, S.E., Park, S.W., Park, C.Y., “Restoration of adiponectin expression via the ERK pathway in TNFα-treated 3T3-L1 adipocytes,” Molecular Medicine Reports, 10(2), 905-10, 2014.
[7]  Abe, Y., Kawakami, A., Osaka, M., Uematsu, S., Akira, S., Shimokado, K., Sacks, F.M., Yoshida, M., “Apolipoprotein CIII induces monocyte chemoattractant protein-1 and interleukin 6 expression via Toll-like receptor 2 pathway in mouse adipocytes,” Arteriosclerosis, thrombosis, and vascular biology, 30(11), 2242-8, 2010.
[8]  Lee, N.Y., Park, K.Y., Min, H.J., Song, K.Y., Lim, Y.Y., Park, J., Kim, B.J., Kim, M.N., “Inhibitory effect of vitamin U (S-methylmethionine sulfonium chloride) on differentiation in 3T3-L1 pre-adipocyte cell lines,” Annals of Dermatology, 24(1), 39-44, 2012.
[9]  Magrone, T., Perez de Heredia, F., Jirillo, E., Morabito, G., Marcos, A., Serafini, M., “Functional foods and nutraceuticals as therapeutic tools for the treatment of diet-related diseases,” Canadian Journal of Physiology and Pharmacology, 91(6), 387-96, 2013.
[10]  Subbaramaiah, K., Sue, E., Bhardwaj, P., Du, B., Hudis, C.A., Giri, D., Kopelovich, L., Zhou, X.K., Dannenberg, A.J., “Dietary polyphenols suppress elevated levels of proinflammatory mediators and aromatase in the mammary gland of obese mice,” Cancer Prevention Research (Philadelphia). 6(9), 886-97, 2013.
[11]  Meydani, M., Hasan, S.T., “Dietary polyphenols and obesity,” Nutrients, 2(7), 737-51, Jul.2010.
[12]  Badescu, M., Badulescu, O., Badescu, L., Ciocoiu, M., “Effects of Sambucus nigra and Aronia melanocarpa extracts on immune system disorders within diabetes mellitus,” Pharmaceutical Biology, 53(4), 533-9, 2015.
[13]  Skarpańska-Stejnborn, A., Basta, P., Sadowska, J., Pilaczyńska-Szcześniak, L., “Effect of supplementation with chokeberry juice on the inflammatory status and markers of iron metabolism in rowers,” Journal of the International Society of Sports Nutrition, 11(1), 48, 2014.
[14]  Tanaka, T., Tanaka, A., “Chemical components and characteristics of Black chokeberry,” Journal of the Japanese Society for Food Science and Technology, 48(8), 606-10, 2001.
[15]  Kokotkiewicz, A., Jaremicz, Z., Luczkiewicz, M., “Aronia plants: a review of traditional use, biological activities, and perspectives for modern medicine,” Journal of Medicinal Food, 13(2), 255-69, 2010.
[16]  Vlachojannis, C., Zimmermann, B.F., Chrubasik-Hausmann, S., “Quantification of anthocyanins in elderberry and chokeberry dietary supplements,” Phytotherapy Research, 29(4), 561-5. 2015.
[17]  Hwang, E.S., Nhuan, D.T., “Antioxidant contents and antioxidant activities of hot-water extracts of aronia (aronia melancocarpa) with different drying methods,” Food Science and Biotechnology, 46(3), 303-8, 2014.
[18]  Lim, J.D., Cha, H.S., Choung, M.G., Choi, R.N., Choi, D.J., Youn, A.R., “Antioxidant activities of acidic ethanol extract and the anthocyanin rich fraction from aronia melanocarpa,” Korean Journal of Food & Cookery Science, 30(5), 573-8, Oct.2014.
[19]  Jurgonski, A., Juskiewicz, J., Zdunczyk, Z., “Ingestion of black chokeberry fruit extract leads to intestinal and systemic changes in a rat model of prediabetes and hyperlipidemia,” Plant Foods for Human Nutrition, 63(4), 176-82, Dec.2008.
[20]  Qin, B., Anderson, R.A., “An extract of chokeberry attenuates weight gain and modulates insulin, adipogenic and inflammatory signalling pathways in epididymal adipose tissue of rats fed a fructose-rich diet,” The British Journal of Nutrition, 108(4), 581-7, 2012.
[21]  Park, H., Liu, Y., Kim, H.S., Shin, J.H., “Chokeberry attenuates the expression of genes related to de novo lipogenesis in the hepatocytes of mice with nonalcoholic fatty liver disease,” Nutrition Research, 36(1), 57-64, 2016.
[22]  Abdali, D., Samson, S.E., Grover, A.K., “How effective are antioxidant supplements in obesity and diabetes?” Medical Principles and Practice, 24(3), 201-15, 2015.
[23]  Rangel-Huerta, O.D., Aguilera, C.M., Martin, M.V., Soto, M.J., Rico, M.C., Vallejo, F., Tomas-Barberan, F., Perez-de-la-Cruz, A.J., Gil, A., Mesa, M.D., “Normal or high polyphenol concentration in orange juice affects antioxidant activity, blood pressure, and body weight in obese or overweight Adults,” The Journal of Nutrition, 145(8), 1808-16, 2015.
[24]  Alharbi, K.K., Syed, R., Khan, I.A., “Computational study on the interaction of flavonoids with fat mass and obesity associated protein,” Journal of Environmental Biology, 36(2), 419-24, 2015.
[25]  Mohammed, A., Al-Numair, K.S., Balakrishnan, A., “Docking studies on the interaction of flavonoids with fat mass and obesity associated protein,” Pakistan Journal of Pharmaceutical Sciences, 28(5), 1647-53, 2015.
[26]  Jung, H.J., “Comparison of Total Polyphenols, Total Flavonoids, and Biological Activities of Black Chokeberry and Blueberry Cultivated in Korea,” Journal of the Korean Society of Food Science and Nutrition, 43(9), 1349-56, Sep.2014.
[27]  Lee, S.L., Lee, H.K., Chin, T.Y., Tu, S.C., Kuo, M.H., Kao, M.C., Wu, Y.C., “Inhibitory effects of purple sweet potato leaf extract on the proliferation and lipogenesis of the 3T3-L1 preadipocytes,” The American Journal of Chinese Medicine, 43(5), 915-25, 2015.
[28]  Fujiwara, M., Mori, N., Sato, T., Tazaki, H., Ishikawa, S., Yamamoto, I., Arai, T., “Changes in fatty acid composition in tissue and serum of obese cats fed a high fat diet,” BMC Veterinary Research, 11, 200, 2015.
[29]  Sun, Q., Chou, G., “Isoflavonoids from Crotalaria albida inhibit adipocyte differentiation and lipid accumulation in 3T3-L1 cells via suppression of PPAR-γ pathway,” PLoS One, 10(8), e0135893, 2015.
[30]  Berger, J.P., “Role of PPARgamma, transcriptional cofactors, and adiponectin in the regulation of nutrient metabolism, adipogenesis and insulin action: view from the chair,” International Journal of Obesity (2005), 29 Suppl 1, S3-S4, 2005.
[31]  Oliveira, D.M., Chalfun-Junior, A., Chizzotti, M.L., Barreto, H.G., Coelho, T.C., Paiva, L.V., Coelho, C.P., Teixeira, P.D., Schoonmaker, J.P., Ladeira, M.M., “Expression of genes involved in lipid metabolism in the muscle of beef cattle fed soybean or rumen-protected fat, with or without monensin supplementation,” Journal of Animal Science, 92(12), 5426-36, 2014.
[32]  Gregoire, F.M., Smas, C.M., Sul, H.S., “Understanding adipocyte differentiation,” Physiological Reviews, 78(3), 783-809. Jul.1998.
[33]  Kim, S.P., Nam, S.H., Friedman, M., “Mechanism of the antiadipogenic-antiobesity effects of a rice hull smoke extract in 3T3-L1 preadipocyte cells and in mice on a high-fat diet,” Food & Function, 6(9), 2939-48, 2015.
[34]  Farr, O.M., Gavrieli, A., Mantzoros, C.S., “Leptin applications in 2015: what have we learned about leptin and obesity?” Current Opinion in Endocrinology, Diabetes, and Obesity, 22(5), 353-9. 2015.
[35]  Lubkowska, A., Dudzińska, W., Bryczkowska, I., Dołęgowska, B., “Body composition, lipid profile, adipokine concentration, and antioxidant capacity changes during interventions to treat overweight with exercise programme and whole-body cryostimulation,” Oxidative Medicine and Cellular Longevity, 2015, 803197, 2015.
[36]  Witzel, A.L., Kirk, C.A., Kania, S.A., Bartges, J.W., Boston, R.C., Moyers, T., Byrd, H., Lauten, S., “Relationship of adiponectin and its multimers to metabolic indices in cats during weight change,” Domestic Animal Endocrinology, 53, 70-7, 2015.
[37]  Cantini, G., Di Franco, A., Samavat, J., Forti, G., Mannucci, E., Luconi, M., “Effect of liraglutide on proliferation and differentiation of human adipose stem cells,” Molecular and Cellular Endocrinology, 402, 43-50, 2015.
[38]  Pan, Z., Wang, H., Liu, Y., Yu, C., Zhang, Y., Chen, J., Wang, X., Guan, Q., “Involvement of CSE/ H2S in high glucose induced aberrant secretion of adipokines in 3T3-L1 adipocytes,” Lipids in Health and Disease, 13, 155, 2014.
[39]  Shen, Y., Song, S.J., Keum, N., Park, T., “Olive leaf extract attenuates obesity in high-fat diet-fed mice by modulating the expression of molecules involved in adipogenesis and thermogenesis,” Evidence-Based. Complementary and Alternative Medicine, 2014, 971890, 2014.