Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2016, 4(4), 210-215
DOI: 10.12691/jfnr-4-4-3
Open AccessArticle

Reduction of Adipogenesis by Chokeberry (Aronia melanocarpa) Extract in 3T3-L1 Preadipocytes

Jung-Hee Shin1 and Ji-Hye Jung2,

1Department of food & nutrition, Joongbu University, Chungnam, Korea

2Institute for Clinical Nutrition, INJE University, Seoul, Korea

Pub. Date: May 16, 2016

Cite this paper:
Jung-Hee Shin and Ji-Hye Jung. Reduction of Adipogenesis by Chokeberry (Aronia melanocarpa) Extract in 3T3-L1 Preadipocytes. Journal of Food and Nutrition Research. 2016; 4(4):210-215. doi: 10.12691/jfnr-4-4-3

Abstract

The aim of this study was investigate the anti-obesity effect and mechanism of action of chokeberry (Aronia melanocarpa) extract (CBE) in 3T3-L1 cells. CBE was tested for its antioxidant compounds content (total phenol and flavonoid). Lipid accumulation levels were measured by determining the expression of genes associated with adipocyte differentiation and adipokine secretion in 3T3-L1 cells. In CBE, the total phenolic content was 212.0 ± 3.11 mg/g and the flavonoids content was 113.25 ± 2.5 mg/g. Oil Red O staining showed that CBE inhibited lipid accumulation at concentration of 0, 0.5, and 1.0 mg/mL in a dose-dependent manner. CBE decreased the expression of the key adipocyte differentiation regulator peroxisome proliferator-activated receptor-γ and the fatty acid binding protein 4 gene during the differentiation of preadipocytes into adipocytes. Moreover, CBE down regulated adipokine-specific genes such as leptin and monocyte chemoattractant protein-1 relative to non-treated adipocytes. These results demonstrated that CBE inhibited adipogenesis by down regulating peroxisome proliferator-activated receptor-γ and fatty acid binding protein 4 and by reducing adipokine expression in 3T3-L1 cells.

Keywords:
chokeberry extract adipocyte differentiation adipokine secretion anti-obesity

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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