Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2013, 1(4), 37-41
DOI: 10.12691/jfnr-1-4-1
Open AccessArticle

Effectiveness of Crowberry on Plasma Total Antioxidant Status, Lipid Profile and Homocysteine

Shin Young Park1 and Sang Pyung Lee2,

1Department of Clinical Pathology, Cheju Halla University, South Korea

2Neuroscience Center, Cheju Halla Hospital, South Korea

Pub. Date: August 16, 2013

Cite this paper:
Shin Young Park and Sang Pyung Lee. Effectiveness of Crowberry on Plasma Total Antioxidant Status, Lipid Profile and Homocysteine. Journal of Food and Nutrition Research. 2013; 1(4):37-41. doi: 10.12691/jfnr-1-4-1


The objective of this study was to assess whether the consumption of crowberry could affect serum lipid profile, homocysteine (Hcy) level, and antioxidant status of healthy subjects. Out of 55 healthy volunteered subjects, 51 completed this investigation to final analysis. Each subject consumed 2 grams of powdered crowberries everyday for four weeks. Crowberry consumption led to significant increase in total antioxidant status (TAS) and superoxide dismutase (SOD), while it resulted in significant decrease in total cholesterol and low-density lipoprotein (LDL) levels. The differences in the levels of antioxidant markers and lipid profiles taken before and after crowberry intake were most significant. The levels of Hcy, catalase, T-cho, triglyceride, and LDL in the higher T-cho group decreased significantly after crowberry intake. Furthermore, this study revealed a significant positive correlation between Hcy level and body weight index (BMI). Crowberry intake improved lipid profile as demonstrated by having decreased T-cho, TG and LDL; increased antioxidative reactions (such as TAS or SOD enzymatic activities) and decreased Hcy levels in healthy subjects.

crowberry antioxidative activity homocysteine lipid profile

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[1]  Basu, A., Rhone, M. and Lyons, T.J, “Berries: emerging impact on cardiovascular health,” Nutrition Reviews, 68(3). 168-177. Mar. 2010.
[2]  Bradley, J.R, TNF-mediated inflammatory disease. Journal of Pathology, 214 (2). 149-160. Jan.2008.
[3]  Xu, H., Uysal, K.T., Becherer, J.D., Arner, P. and Hotamisligil, G.S, Altered tumor necrosis factor-α (TNF-α) processing in adipocytes and increased expression of transmembrane TNF-α in Obesity. Diabetes, 51 (6). 1876-1883. Jun.2002.
[4]  Nagao, T., Hase, T. and Tokimitsu, I, “A green tea extract high in catechins reduces body fat and cardiovascular risks in humans,” Obesity, 15(6). 1473-1483. Jun. 2007.
[5]  Zaveri, N.T, “Green tea and its polyphenolic catechins: medicinal uses in cancer and noncancer applications,” Life Sciences, 78(18). 2073-2080. Mar. 2006.
[6]  Black, R.A., Rauch, C.T., Kozlosky, C.J., Peschon J.J., Slack, J.L., Wolfson, M.F., Castner, B.J., Stocking, K.L., Reddy, P., Srinivasan, S., Nelson, N., Boiani, N., Schooley, K.A., Gerhart, M., Davis, R., Fitzner, J.N., Johnson, R.S., Paxton, R.J., March, C.J. and Cerretti, D.P, A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature, 385 (6618). 729-733. Feb.1997.
[7]  Horiuchi, T., Mitoma, H., Harashima, S., Tsukamoto, H. and Shimoda, T, Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents. Rheumatology (Oxford), 49 (7). 1215-1228. Jul.2010.
[8]  Bosetti, C., Spertini, L., Parpinel, M., Gnagnarella, P., Lagiou, P., Negri, E., Franceschi, S., Montella, M., Peterson, J., Dwyer, J., Giacosa, A. and La Vecchia, C, “Flavonoids and breast cancer risk in Italy,” Cancer Epidemiology, Biomarkers&Prevention, 14(4). 805-808. Apr. 2005.
[9]  Riboli, E. and Norat, T, “Epidemiologic evidence of the protective effect of fruit and vegetables on cancer risk,” The American Journal of Clinical Nutrition, 78(3 suppl.). 559S-569S. Sep. 2003.
[10]  Calzascia, T., Pellegrini, M., Hall, H., Sabbagh, L., Ono, N., Elford, A.R., Mak, T.W. and Ohashi, P.S, TNF-α is critical for antitumor but not antiviral T cell immunity in mice. Journal of Clinical Investigation, 117 (12). 3833-3845. Dec.2007.
[11]  Van Horssen, R., Ten Hagen, T.L. and Errgermont, A.M, TNF-alpha in cancer treatment: molecular insights, antitumor effects, and clinical utility. Oncologist, 11 (4). 397-408. Apr.2006.
[12]  Rietveld, A. and Wiseman, S, “Antioxidant effects of tea: evidence from human clinical trials,” The Journal of Nutrition, 133(10). 3285S-3292S. Oct. 2003.
[13]  Moselhy, S.S. and Demerdash, S.H, “Plasma homocysteine and oxidative stress in cardiovascular disease,” Disease Markers, 19(1). 27-31. Dec. 2003.
[14]  Zykova, S.N., Svartberg, J., Seljelid, R., Iversen, H., Lund, A., Svistounov, D.N. and Jenssen, T.G, Release of TNF-alpha from in vitro-stimulated monocytes is negatively associated with serum levels of apolipoprotein B in patients with type 2 diabetes. Scandinavian Journal of Immunology, 60 (5). 535-542. Nov.2004.
[15]  Braun, S., Bitton-Worms, K. and LeRoith, D, The link between the metabolic syndrome and cancer. International Journal of Biological Sciences, 7 (7). 1003-1015. 2011.
[16]  Tyagi, N., Sedoris, K.C., Steed, M., Ovechkin, A.V., Moshal, K.S. and Tyagi, S.C, “Mechanisms of homocysteine-induced oxidative stress,” American Journal of Physiology. Heart and Circulatory Physiology, 289(6). H2649-2656. Dec. 2005.
[17]  Park, S.Y., Lee, E.S., Han, S.H., Lee, H.Y. and Lee, S.J, "Antioxidative effects of two native berry species, Empetrum nigrum Var. Japonicum K. Koch and Rubus buergeri Miq., from the Jeju Island of Korea", Journal of Food Biochemistry, 36(6). 675-682. Dec. 2012.
[18]  Grundy, S.M., Brewer, H.B. Jr., Cleeman, J.I., Smith, S.C. Jr., Lenfant, C., American Heart Association and National Heart, Lung, and Blood Institute, Definition of metabolic syndrome: Report of the National Heart, Lung, and Blood Institute/American Heart Association conference on scientific issues related to definition. Circulation, 109 (3). 433. Jan.2004.
[19]  Geerlings, S.E., Brouwer, E.C., Van Kessel, K.C., Gaastra, W., Stolk, R.P. and Hoepelman, A.I, Cytokine secretion is impaired in women with diabetes mellitus. European Journal of Clinical Investigation, 30 (11). 995-1001. Nov.2000.
[20]  Mezesova, L., Bartekova, M., Javorkova, V., Vlkovicova, J., Breier, A. and Vrbjar, N, “Effect of quercetin on kinetic properties of renal Na,K-ATPase in normotensive and hypertensive rats,” Journal of Physiology and Pharmacology, 61(5). 593-598. Oct. 2010.
[21]  Ogawa, K., Sakakibara, H., Iwata, R., Ishii, T., Sato, T., Goda, T., Shimoi, K. and Kumazawa, S, “Anthocyanin composition and antioxidant activity of the Crowberry (Empetrum nigrum) and other berries,” Journal of Agricultural and Food Chemistry, 56(12). 4457-4462. Jun. 2008.
[22]  Miyazaki, Y., Pipek, R., Mandarino, L.J. and DeFronzo, R.A, Tumor necrosis factor alpha and insulin resistance in obese type 2 diabetic patients. International Journal of Obesity and Related Metabolic Disorders, 27 (1). 88-94. Jan.2003.
[23]  Li, Q., Li, L., Shi, W., Jiang, X., Xu, Y., Gong, F., Zhou, M., Edwards, C.K. 3rd. and Li, Z, Mechanism of action differences in the antitumor effects of transmembrane and secretory tumor necrosis factor-alpha in vitro and in vivo. Cancer Immunology, Immunotherapy, 55 (12). 1470-1479. Dec.2006.
[24]  Cao, G. and Prior, R.L, “Comparison of different analytical methods for assessing total antioxidant capacity of human serum,” Clinical Chemistry, 44(6). 1309-1315. Jun. 1998.
[25]  Defuria, J., Bennett, G., Strissel, K.J., Perfield, J.W., 2nd, Milbury, P.E., Greenberg, A.S. and Obin, M.S, “Dietary blueberry attenuates whole-body insulin resistance in high fat-fed mice by reducing adipocyte death and its inflammatory sequelae,” The Journal of Nutrition, 139(8). 1510-1516. Aug. 2009.
[26]  Maggini, S., Wintergerst, E.S., Beveridge, S. and Hornig, D.H, Selected vitamins and trace elements support immune function by strengthening epithelial barriers and cellular and humoral immune responses. British Journal of Nutrition, 98 (Suppl 1). S29-S35. Oct.2007.
[27]  Grimble, R.F, Nutritional modulation of immune function. Proceedings of Nutrition Society, 60 (3), 389-397. Aug.2001.
[28]  Prior, R.L., Wu, X., Gu, L., Hager, T.J., Hager, A. and Howard, L.R, “Whole berries versus berry anthocyanins: interactions with dietary fat levels in the C57BL/6J mouse model of obesity,” Journal of Agricultural and Food Chemistry, 56(3). 647-653. Feb. 2008.
[29]  Noda, Y., Kaneyuki, T., Mori, A. and Packer, L, “Antioxidant activities of pomegranate fruit extract and its anthocyanidins: delphinidin, cyanidin, and pelargonidin,” Journal of Agricultural and Food Chemistry, 50(1). 166-171. Jan. 2002.
[30]  Cole, C.R. and Lifshitz, F, Zinc nutrition and growth retardation. Pediatric Endocrinology Review, 5 (4). 889-896. Jun.2008.
[31]  Bao, B., Prasad, A.S., Beck, F.W. and Godmere, M, Zinc modulates mRNA levels of cytokines. American Journal of Physiology. Endocrinology Metabolism, 285 (5). E1095- E1102. Nov.2003.
[32]  Heinonen, M, “Antioxidant activity and antimicrobial effect of berry phenolics--a Finnish perspective,” Molecular Nutrition & Food Research, 51(6). 684-691. Jun. 2007.
[33]  Wu, X., Kang, J., Xie, C., Burris, R., Ferguson, M.E., Badger, T.M. and Nagarajan, S, “Dietary blueberries attenuate atherosclerosis in apolipoprotein E-deficient mice by upregulating antioxidant enzyme expression,” The Journal of Nutrition, 140(9). 1628-1632. Sep. 2010.
[34]  Umeta, M., West, C.E., Haidar, J., Deurenberg, P. and Hautvast, J.G, Zinc supplementation and stunted infants in Ethiopia: a randomized controlled trial. Lancet, 355 (9220). 2021-2026. Jun.2000.
[35]  Shay, N.F. and Mangian, H.F, Neurobiology of zinc-influenced eating behavior. Journal of Nutrition, 130 (5S Suppl). 1493S- 1499S. May.2000.
[36]  Chen, P., Chien, K., Su, T., Chang, C., Liu, T., Cheng, H. and Tsai, C, “Dietary sesame reduces serum cholesterol and enhances antioxidant capacity in hypercholesterolemia,” Nutrition Research, 25(6). 559-567. Jun. 2005.
[37]  Mccully, K.S, 1969. “Vascular pathology of homocysteinemia: implications for the pathogenesis of homocysteinemia: implications for the pathogenesis of arteriosclerosis,” The American Journal of Pathology, 56(1). 111-128. Jul. 1969.
[38]  Ibs, K.H. and Rink, L, Zinc-Altered Immune Function. Journal of Nutrition, 133 (5 Suppl 1). 1452S-1456S. May.2003.
[39]  Haase, H., Ober-Blobaum, J.L., Engelhardt, G., Hebel, S., Heit, A., Heine, H. and Rink, L, Zinc signals are essential for lipopolysaccharide-induced signal transduction in monocytes. Journal of Immunology, 181 (9). 6491-6502. Nov.2008.
[40]  Targher, G., Bertolini, L., Zenari, L., Cacciatori, V., Muggeo, M., Faccini, G. and Zoppini, G, “Cigarette smoking and plasma total homocysteine levels in young adults with type 1 diabetes,” Diabetes Care, 23(4). 524-528. Apr. 2000.
[41]  Blom, H.J, “Determinants of plasma homocysteine,” The American Journal of Clinical Nutrition, 67(2). 188-189. Feb. 1998.
[42]  Bao, B., Prasad, A.S., Beck, F.W., Snell, D., Suneja, A., Sarkar, F.H., Doshi, N., Fitzgerald, J.T. and Swerdlow, P, Zinc supplementation decreases oxidative stress, incidence of infection, and generation of inflammatory cytokines in sickle cell disease patients. Translational Research, 152 (2). 67-80. Aug.2008.
[43]  Baum, M.K., Lai, S., Sales, S., Page, J.B. and Campa, A, Randomized, controlled clinical trial of zinc supplementation to prevent immunological failure in HIV-infected adults. Clinical Infectious Diseases, 50 (12). 1653-1660. Jun.2010.
[44]  Bergmark, C., Mansoor, M.A., Svardal, A. and De Faire, U, “Redox status of plasma homocysteine and related aminothiols in smoking and nonsmoking young adults,” Clinical Chemistry, 43(10). 1997-1999. Oct. 1997.
[45]  Prior, R.L., Wu, X., Gu, L., Hager, T.J., Hager, A. and Howard, L.R, “Whole berries versus berry anthocyanins: interactions with dietary fat levels in the C57BL/6J mouse model of obesity,” Journal of Agricultural and Food Chemistry, 56(3). 647-653. Feb. 2008.
[46]  Yalçın, S.S., Engür-Karasimav, D., Alehan, D., Yurdakök, K., Ozkutlu, S. and Coşkun, T, Zinc supplementation and TNF-α levels in vaccinated cardiac patients. Journal of Trace Elements in Medicine and Biology, 25 (2). 85-90. Apr.2011.
[47]  Aydemir, T.B., Blanchard, R.K. and Cousins, R.J, Zinc supplementation of young men alters metallothionein, zinc transporter, and cytokine gene expression in leukocyte populations. Proceedings of the National Academy of Sciences of the United State of America, 103 (6). 1699-1704. Feb.2006.
[48]  Manach, C., Mazur, A. and Scalbert, A, “Polyphenols and prevention of cardiovascular diseases,” Current Opinion in Lipidology, 16(1). 77-84. Feb. 2005.
[49]  Alberti, K.G., Zimmet, P., Shaw, J, IDF Epidemiology Task Force Consensus Group. The metabolic syndrome--a new worldwide definition. Lancet, 366 (9491). 1059-1062. Sep.2005.
[50]  Aprikian, O., Duclos, V., Guyot, S., Besson, C., Manach, C., Bernalier, A., Morand, C., Remesy, C. and Demigne, C, “Apple pectin and a polyphenol-rich apple concentrate are more effective together than separately on cecal fermentations and plasma lipids in rats,” The Journal of Nutrition, 133(6). 1860-186.
[51]  Brown, K.H, Effect of infections on plasma zinc concentration and implications for zinc status assessment in low-income countries. American Journal of Clinical Nutrition, 68 (2 Suppl). 425S-429S. Aug.1998.
[52]  Al-Maroof, R.A. and Al-Sharbatti, S.S, Serum zinc levels in diabetic patients and effect of zinc supplementation on glycemic control of type 2 diabetics. Saudi Medical Journal, 27 (3). 344-350. Mar.2006.
[53]  Chausmer, A.B, Zinc, insulin and diabetes. Journal of American College of Nutrition, 17 (2). 109-115. Apr.1998.
[54]  Soinio, M., Marniemi, J., Laakso, M., Pyörälä, K., Lehto, S. and Rönnemaa, T, Serum zinc level and coronary heart disease events in patients with type 2 diabetes. Diabetes Care, 30 (3). 523-528. Mar.2007.
[55]  Martin, J.H. and Edwards, S.W, Changes in mechanisms of monocyte/macrophage-mediated cytotoxicity during culture. Reactive oxygen intermediates are involved in monocyte-mediated cytotoxicity, whereas reactive nitrogen intermediates are employed by macrophages in tumor cell killing. Journal of Immunology, 150 (8 Pt 1). 3478-3486. Apr.1993.
[56]  Haffner, S.M, Risk constellations in patients with the metabolic syndrome: epidemiology, diagnosis, and treatment patterns. American Journal of Medicine, 119 (5 Suppl 1). S3-S9. May.2006.
[57]  Komura,T., Sakai, Y., Honda, M., Takamura, T., Matsushima, K. and Kaneko, S, CD14+ monocytes are vulnerable and functionally impaired under endoplasmic reticulum stress in patients with type 2 diabetes. Diabetes, 59 (3). 634-643. Mar.2010.
[58]  Alexandraki, K.I., Piperi, C., Ziakas, P.D., Apostolopoulos, N.V., Makrilakis, K., Syriou, V., Diamanti-Kandarakis, E., Kaltsas, G. and Kalofoutis, A, Cytokine secretion in long-standing diabetes mellitus type 1 and 2: associations with low-grade systemic inflammation. Journal of Clinical Immunology, 28 (4). 314-321. Jul.2008.