Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2016, 4(2), 69-75
DOI: 10.12691/jfnr-4-2-1
Open AccessArticle

Effect of Oat Particle Concentration and Size Distribution on the Phase Behaviour of Mixtures with Gelatin

Nashi K. Alqahtani1, John Ashton2, Lita Katopo1, , Oliver A.H. Jones1 and Stefan Kasapis1

1School of Science, RMIT University, Melbourne, Vic 3001, Australia

2Sanitarium Development and Innovation, Sanitarium Health and Wellbeing Company, Cooranbong, NSW 2265, Australia

Pub. Date: February 17, 2016

Cite this paper:
Nashi K. Alqahtani, John Ashton, Lita Katopo, Oliver A.H. Jones and Stefan Kasapis. Effect of Oat Particle Concentration and Size Distribution on the Phase Behaviour of Mixtures with Gelatin. Journal of Food and Nutrition Research. 2016; 4(2):69-75. doi: 10.12691/jfnr-4-2-1


The present study examines the effect of oat particle addition on the structural properties of gelatin gels. In doing so, gelatin concentration was 2% (w/w) and a variable amount of oat (0-4%, w/w) was employed. The latter came at three different particle size distributions, i.e. 28.2, 82.9, and 182.2 μm. Mechanical observations were carried out using small deformation dynamic oscillation in shear alongside thermal studies with micro differential scanning calorimetry. Scanning electron microscopy images provided tangible evidence of the changing morphology in the binary mixture with the addition of oat particles. Phase separated matrices are formed where gelatin is the continuous phase supporting the discontinuous inclusions of oat particles. There was an immediate decrease in the mechanical strength of the composite gel with the addition of oat particles, which was accompanied by a parallel drop in the enthalpy values of helical associations in gelatin. Increasing concentrations of oat with the smallest particle-size distribution are capable of disturbing rapidly the gelatin network, as compared to the larger counterparts.

oat particles dietary fibre gelatin particle size distribution phase separation

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[1]  Codex Alimentarius, Guidelines on nutrition labelling (2013).
[2]  Cho SS and Prosky L, Complex carbohydrates: Definition and analysis, in Complex carbohydrates, ed by Cho SS, Prosky L and Dreher M. Marcel Dekker, New York, (1999).
[3]  Slavin JL, Jacobs D. and Marquart L, Grain Processing and Nutrition. Crit Rev Food Sci Nutr 40: 309-326 (2000).
[4]  Guillon F and Champ M, Structural and physical properties of dietary fibres, and consequences of processing on human physiology. Food Res Int 33: 233-245 (2000).
[5]  Chau CF, Wen YL and Wang YT, Improvement of the functionality of a potential fruit insoluble fibre by micron technology. Int J Food Sci Technol 41: 1054-1060 (2006).
[6]  Alqahtani NK, Ashton J, Katopo L, Haque E, Jones OAH and Kasapis S, Consistency of UHT beverages enriched with insoluble fibre during storage. Bioactive Carbohydrates and Dietary Fibre 4: 84-92 (2014).
[7]  Anderson JW, Smith BM and Gustafson NJ, Health benefits and practical aspects of high-fiber diets. Am J Clin Nutr 59: 1242S-1247S (1994).
[8]  Tinker LF, Schneeman BO, Davis PA, Gallaher DD and Waggoner CR, Consumption of prunes as a source of dietary fiber in men with mild hypercholesterolemia. Am J Clin Nutr 53: 1259-1265 (1991).
[9]  Collar C and Angioloni A, An approach to structure-function relationships of polymeric dietary fibres in foods: significance in breadmaking applications, in Dietary Fibre: New Frontiers For Food and Health, ed by van der Kamp JW and McCleary BV. Wageningen Academic Publishers, Wageningen (2010).
[10]  Narchi I, Vial C and Djelveh G, Effect of protein–polysaccharide mixtures on the continuous manufacturing of foamed food products. Food Hydrocoll 23: 188-201 (2009).
[11]  Schrieber R and Gareis H, Gelatine handbook - theory and industrial practice. Wiley-VCH Verlag, Weinheim, Germany (2007).
[12]  Haug IJ and Draget KI, Gelatin, in Handbook of Food Protein, ed by Phillips GO and Williams PA. Woodhead Publishing, Cambridge (2011).
[13]  Almrhag O, George P, Bannikova A, Katopo L, Chaudhary D and Kasapis S, Investigation on the phase behaviour of gelatin/agarose mixture in an environment of reduced solvent quality. Food Chem 136: 835-842 (2013).
[14]  Fang Y, Li L, Inoue C, Lundin L and Appelqvist I, Associative and Segregative Phase Separations of Gelatin/κ-Carrageenan Aqueous Mixtures. Langmuir 22: 9532-9537 (2006).
[15]  Harrington JC and Morris ER, Conformational ordering and gelation of gelatin in mixtures with soluble polysaccharides. Food Hydrocoll 23: 327-336 (2009).
[16]  Katopo L, Kasapis S and Hemar Y, Segregative phase separation in agarose/whey protein systems induced by sequence-dependent trapping and change in pH. Carbohydr Polym 87: 2100-2108 (2012).
[17]  Anderson VJ and Jones RAL, The influence of gelation on the mechanism of phase separation of a biopolymer mixture. Polymer 42: 9601-9610 (2001).
[18]  Richardson RK, Robinson G, Ross-Murphy SB and Todd S, Mechanical spectroscopy of filled gelatin gels. Polymer Bull 4: 541-546 (1981).
[19]  Koh LW and Kasapis S, Orientation of short microcrystalline cellulose fibers in a gelatin matrix. Food Hydrocoll 25: 1402-1405 (2011).
[20]  Oomah BD, Mazza G. and Przybylski R, Comparison of flaxseed meal lipids extracted with different solvents. LWT-Food Sci Technol 29:654-658 (1996).
[21]  Bunzel M, Ralph J, Marita JM, Hatfield RD and Steinhart H, Diferulates as structural components in soluble and insoluble cereal dietary fibre. J Sci Food Agric 84: 653-660 (2001).
[22]  Djabourov M, Lechaire JP, and Gaill F, Structure and rheology of gelatin and collagen gels. Biorheology 30: 191 (1993).
[23]  Hawkins K, Lawrence M, Williams PR, and Williams RL, A study of gelatin gelation by Fourier transform mechanical spectroscopy. J Non-Newtonian Fluid Mech 148: 127-133 (2008).
[24]  Fonkwe LG, Narsimhan G, and Cha AS, Characterization of gelation time and texture of gelatin and gelatin–polysaccharide mixed gels. Food Hydrocoll 17: 871-883 (2003).
[25]  Tabilo-Munizaga G and Barbosa-Canovas GV, Rheology for the food industry. J Food Eng 67: 147-156 (2005).
[26]  Kasapis S, Phase separation in biopolymer gels: a low- to high-solid exploration of structural morphology and functionality. Crit Rev Food Sci Nutr 48: 341-359 (2008).
[27]  Gilsenan PM and Ross-Murphy SB, Viscoelasticity of thermoreversible gelatin gels from mammalian and piscine collagens. J Rheol 44: 871-883 (2000).
[28]  Strange ED and Onwulata CI, Effect of particle size on the water sorption properties of cereal fibers. J Food Qual 25: 63-73 (2002).
[29]  Mousia Z, Farhat IA, Blachot JF and Mitchell JR, Effect of water partitioning on the glass-transition behaviour of phase separated amylopectin–gelatin mixtures. Polymer 41: 1841-1848 (2000).
[30]  Rahman MS, State diagram of foods: Its potential use in food processing and product stability. Trends Food Sci Technol 17: 129-141 (2006).
[31]  McElhaney RN, Differential scanning calorimetric studies of lipid-protein interactions in model systems. Biochim Biophys Acta 864: 361-421 (1986).
[32]  Harwalkar VR and Ma CY, Study of thermal properties of oat globulin by differential scanning calorimetry. J of Food Sci 52: 394-398 (1987).
[33]  Stevenson DG, Eller FJ, Jane J, and Inglett GE, Structure and physicochemical properties of defatted and pin-milled oat bran concentrate fractions separated by air-classification. Int J Food Sci Technol 43: 995-1003 (2008).
[34]  Almrhag O, George P, Bannikova A, Katopo L, Chaudhary D, and Kasapis S, Phase behaviour of gelatin/polydextrose mixtures at high level of solids. Food Chem 134: 1938-1946 (2012).
[35]  Djabourov M, Leblond J and Papon P, Gelation of aqueous gelatin solutions. I. Structural investigation. J Phys 49: 319-332 (1988).