Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2015, 3(10), 629-636
DOI: 10.12691/jfnr-3-10-2
Open AccessArticle

Antioxidant Properties and Color Stability of Anthocyanin Purified Extracts from Thai Waxy Purple Corn Cob

Benjaruk Vayupharp1 and Varaporn Laksanalamai1,

1Faculty of Food Technology Rangsit University, Meang-Ake Pahtumthani, Thailand

Pub. Date: December 03, 2015

Cite this paper:
Benjaruk Vayupharp and Varaporn Laksanalamai. Antioxidant Properties and Color Stability of Anthocyanin Purified Extracts from Thai Waxy Purple Corn Cob. Journal of Food and Nutrition Research. 2015; 3(10):629-636. doi: 10.12691/jfnr-3-10-2


The extraction, purification, and identification of extracted anthocyanins from Thai waxy purple corn cob were determined. Anthocyanins and total phenol content were prepared by 0.01% HCl- water and 0.01% HCl-ethanol extractions. The ratios of 1:20 and 1:100 (solid: solvent) for 90 mins were the proper extraction conditions to obtain the maximum amount of anthocyanin extracts and the optimum amount of total phenol content as extracted by acidified water and acidified ethanol, respectively. The antioxidant activities of the anthocyanin extract measured by DPPH radical-scavenging activities and ferric reducing antioxidant powers (FRAP) were in a range of 6.51 mM ascorbic acid /100g-6.29 mM ascorbic acid /100g, and 5.34 mM ascorbic acid /100g-4.68 mM ascorbic acid /100g, respectively. A profile of the anthocyanin extracts identified by LC-MS was composed of 7 major anthocyanins; (1) cyanidin-3-glucoside, (2) pelargonidin-3-glucoside, (3) peonidin-3-glucoside, (4) cyanidin-3-(6-malonylglucoside), (5) pelargonidin-3-(6-malonylglucoside), (6) peonidin-3-(6-malonylglucoside) and (7) cyanidin-3-(6-ethylmalonylglucoside). The color of the anthocyanin extracts was dependent upon pH which gradually changed from dark red to dark brown when pH increased from 1 to 9. An increase in the degradation rate constant (k) with a corresponding decline in the t1/2 values was observed with the increasing temperature at pH 1 and 4. Due to the effect of pH and temperature on the stability of the anthocyanin extract from Thai waxy purple corn cob with the consequence of color changes, it is preferable to apply anthocyanin extract in acidic foods at low temperature to assure stability of color in the products.

anthocyanins antioxidant properties color stability Thai waxy corn cob

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[1]  Cevallos-Casals, B.A. and Cisneros-Zevallos, L. Stability of anthocyanin-based aqueous extracts of Andean purple corn and red-fleshed sweet potato compared to synthetic and natural colorants. Food Chemistry 86(1): 69-77. June 2004.
[2]  Cisse, M., Bohuon, P., Sambe, F., Kan, C., Sakho, M., and Dornier, M. Aqueous extraction of anthocyanins from Hibiscus sabdariffa: Experimental kinetics and modeling. Journal of Food Engineering 109:16-21.March 2012.
[3]  de Pascual-Teresa, S., Santos-Buelga, C., and Rivas-Gonzalo, J.C. LC-MS analysis of anthocyanins from purple corn cob. Journal of the Science of Food and Agriculture 82: 1003-1006. July 2002.
[4]  Fan, G., Han, Y., Gu, Z., and Gu, F. Composition and colour stability of anthocyanins extracted from fermented purple sweet potato culture. LWT-Food Science and Technology 41: 1412-1416.November 2008.
[5]  Hillebrand, S., Naumann, H., Kitzinski, N., Kőhler, N., Winterhalter, P. Isolation and characterization of anthocyanins from blue-fleshed potatoes (Solanum tuberosum L.). In Yee, N. and Bussell, W. T. (Eds.) Potato III Food 3 (Special Issue1), 2009, 96-101.
[6]  Jie, L., Xio-ding, L., Yun, Z., Zheng-dong, Z., Zhi-ya, Q., Meng, L., Shao-hua, Z., Shuo, L., Meng, W., and Lu, Q. Identification and thermal stability of purple-fleshed sweet potato anthocyanins in aqueous solutions with various pH values and fruit juices. Food Chemistry 136: 1429-1434. February 2013.
[7]  Longo, L., and Vasapollo, G. Extraction and identification of anthocyanins from Smilax aspera L. berries. Food Chemistry 94(2): 226-231. January 2006.
[8]  MoBhammer, M.R., Stintzing, F.C., and Carle, R. Evaluation of different methods for the production of juice concentrates and fruit powders from cactus pear. Innovative Food Science and Engineering Technologies 7(4): 275-287. December 2006.
[9]  Moreno, Y.S., Sanchez, G.S., Hernandez, D.R., and Lobato, N.R. Characterization of anthocyanin extracts from maize kernels. Journal of Chromatographic Science 43(9): 483-487. October 2005.
[10]  Obón, J.M., Castellar, M.R., Alacid, M., and Fernandez-Ló, J.A. Production of a red-purple food colorant from Opuntia stricta fruits by spray drying and its application in food model systems. Journal of Food Engineering 90(4): 471-479. February 2009.
[11]  Sun, J., Yao, J., Huang, S., Long, X., Wang, J., and García-García, E. Antioxidant activity of polyphenol and anthocyanin extracts from fruits of Kadsura coccinea (Lem.). Food Chemistry 117(2): 276-281. November 2009.
[12]  Tian S., Nakamura, K., and Kayahara, H. Analysis of phenolic compounds in white rice, brown rice, and germinated brown rice. Journal of Agricultural Food Chemistry 52(15): 4808-4813. July 2004.
[13]  Todaro, A., Cimino, F., Rapisarda, P., Catalano, A.E., Barbagallo, R.N., and Spagna, G. Recovery of anthocyanins from eggplant peel. Food Chemistry 114(2): 434-439. May 2009.
[14]  Wrolstad, R.E., Acree, T.E., Decker, E.A., Penner, M.H., Reid, D.S., Schwartz, S.J., Shoemaker, C.F., Smith, D.M., and Sporns, P. Pigments and Colorants F 1-119. In Handbook of Food Analytical Chemistry. John Wiley& Sons, Inc. Canada. 2000-2005.
[15]  Yang, Z., and Zhai, W. Identification and antioxidant activity of anthocyanins extracted from the seed and cob of purple corn (Zea mays L.). Innovative Food Science and Emerging Technologies 11(1): 169-176.January 2010.
[16]  Yang, Z., and Zhai, W. Optimization of microwave-assisted extraction of anthocyanins from purple corn (Zea mays L.) cob and identification with HPLC-MS. Innovative Food Science and Emerging Technologies 11(3): 470-476. July 2010.
[17]  Zhaohua, H., Qin, P., and Ren, G. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. Japonica) on chronically alcohol-induced liver damage in rats. Journal of Agricultural Food Chemistry 58(5): 3191-3196. March 2010.