Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2015, 3(8), 526-539
DOI: 10.12691/jfnr-3-8-8
Open AccessArticle

Proteomic Identification of Stonefish Synanceja verrucosa Venom

Tai-Yuan Chen1, Yu-Huai Chang1, Hsi-Pin Lin2, Shui-Tein Chen3 and Deng-Fwu Hwang1, 4,

1Department of Food Science and Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan, ROC

2Department of Food Science, Yuanpei University, Hsinchu, Taiwan, ROC

3Institute of Biological Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan, ROC

4Department of Health and Nutrition, Asia University, Taichung, Taiwan, ROC

Pub. Date: October 29, 2015

Cite this paper:
Tai-Yuan Chen, Yu-Huai Chang, Hsi-Pin Lin, Shui-Tein Chen and Deng-Fwu Hwang. Proteomic Identification of Stonefish Synanceja verrucosa Venom. Journal of Food and Nutrition Research. 2015; 3(8):526-539. doi: 10.12691/jfnr-3-8-8

Abstract

Three venom toxins, neoverrucotoxin (neoVTX) α-subunit and β-subunit as well as verrucotoxin (VTX) β-subunit, were identified in the stonefish Synanceja verrucosa by SDS-PAGE, Native-PAGE and two-dimensional electrophoresis (2-DE) coupled with Matrix Assisted Laser Desorption Ionization-Quadrupole-Time-of-Flight (MALDI-Q-TOF). The venom estimated by Native-PAGE were 471, 358, 260 and 166 kDa. The predominate protein bands of crude venom were 84 and 75 kDa by SDS-PAGE. The crude venom protein fell in the region with pI values of 7-9 and molecular weights of 75-90 kDa by 2-DE. Peptide mass fingerprints (PMF) and MS/MS ions originated from MALDI-Q-TOF were used to identify the protein. Our results showed that the complete components of neoverrucotoxin (neoVTX) α-subunit and β-subunit as well as verrucotoxin (VTX) β-subunit were identified from SDS-PAGE and 2-DE patterns. Native-PAGE did not yield protein identifications but revealed the presence of protein complexes.

Keywords:
2-DE MALDI-Q-TOF neoverrucotoxin spine venom Synanceja verrucosa

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