Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2015, 3(7), 437-444
DOI: 10.12691/jfnr-3-7-5
Open AccessArticle

Bioactivity of β-1,3-xylan Extracted from Caulerpa lentillifera by Using Escherichia coli ClearColi BL21(DE3)-β-1,3-xylanase XYLII

Wen-Sing Liang1, 2, Tristan C. Liu1, Chun-Ju Chang1 and Chorng-Liang Pan1, 2,

1Department of Food Science, National Taiwan Ocean University, Jhongjheng District, Keelung City, Taiwan

2Taiwan Algae Research Center, National Taiwan Ocean University, Jhongjheng District, Keelung City, Taiwan

Pub. Date: September 09, 2015

Cite this paper:
Wen-Sing Liang, Tristan C. Liu, Chun-Ju Chang and Chorng-Liang Pan. Bioactivity of β-1,3-xylan Extracted from Caulerpa lentillifera by Using Escherichia coli ClearColi BL21(DE3)-β-1,3-xylanase XYLII. Journal of Food and Nutrition Research. 2015; 3(7):437-444. doi: 10.12691/jfnr-3-7-5


Oligosaccharides extracted from algae exhibit many bioactivities and are used as food additives and dietary supplements. In this study, β-1,3-xylan was extracted from the green algae Caulerpa lentillifera; this compound was hydrolyzed by β-1,3-xylanase XYLII to produce mixed < 3 kDa β-1,3-xylooligosaccharide (XOSmix), which was mainly composed of β-1,3-xylose, β-1,3-xylobiose, and β-1,3-xylotriose. The antioxidant and anticoagulant activities of XOSmix were then examined. Results revealed that the 2,2-diphenyl-1-pikryl-hydrazyl scavenging activity, reducing power, and total antioxidant status of 20 mg/mL XOSmix was equivalent to those of 8.7, 115.1, and 157.3 μg/mL trolox, respectively; whereas the ferrous ion chelating activity of 20 mg/mL XOSmix was equivalent to that of 64.3 μg/mL EDTA. Regarding the anticoagulant activity, XOSmix delayed the activated partial thromboplastin time. These results suggest that XOSmix exhibits potential for application in the food industry.

pseudomonas vesicularis MA103 β-13-xylanase β-13-xylooligosaccharide bioactivity

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