Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Go
Journal of Food and Nutrition Research. 2015, 3(4), 281-284
DOI: 10.12691/jfnr-3-4-8
Open AccessArticle

Inhibitory Effect of Cancer Cells Proliferation from Epigallocatechin-3-O-gallate

Hyun Woo Kang1,

1Department of Korean Food & Culinary Arts, Youngsan University, Busan, Korea

Pub. Date: April 27, 2015

Cite this paper:
Hyun Woo Kang. Inhibitory Effect of Cancer Cells Proliferation from Epigallocatechin-3-O-gallate. Journal of Food and Nutrition Research. 2015; 3(4):281-284. doi: 10.12691/jfnr-3-4-8

Abstract

Epigallocatechin-3-O-gallate (EGCG), the major polyphenol of green tea and a functional food ingredient/nutraceutical with health-promoting properties. However, its anti-cancer activities on various cancer cells are still little information. Here, we show that anti-cancer activities of the EGCG were evaluated using apoptosis assays analyzed by flow cytometry. The inhibitory activities of proliferation in MPC-11, Caco-2, and MCF-7 cells, values were 66.2, 60.3, and 74.8% at 10 μM, respectively. In addition, in an flow cytometry assay on the MPC-11, Caco-2, and MCF-7 cells, the EGCG showed a cell apoptosis effect on cancer/tomor in vitro model. Our results indicate that EGCG has anti-cancer activities against human lung cancer cells through inducing cell cycle arrest, DNA damage and activating mitochondrial signal pathway. These results indicate that EGCG effectively inhibits in vitro tumor growth by inducing apoptosis of cancer cells.

Keywords:
EGCG cancer/tumor cell apoptosis flow cytometer

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Lee, S.J., Kang, H.W., Lee, S.Y., and Hur, S.J., “Green tea polyphenol epigallocatechin-3-O-gallate attenuates lipopolysaccharide-induced nitric oxide production in RAW264.7 cells” J Food Nutr Res 2(7), 425-428. 2014.
 
[2]  MacMicking, J., Xie, Q.W., and Nathan, C., “Nitric oxide and macrophage function”, Annu Rev Immunol 15, 323-350. 1997.
 
[3]  Schoedon, G., Schneemann, M., Walter, R., Blau, N., Hofer, S., and Schaffner, A., “Nitric oxide and infection: another view” Clin Infect Dis 21(Suppl 2), S152-157. 1995.
 
[4]  Forman, H.J., and Torres, M., “Redox signaling in macrophages” Mol Aspects Med 22(4-5), 189-216. 2001.
 
[5]  Kim, S., and Ponka, P., “Nitric oxide-mediated modulation of iron regulatory proteins: implication for cellular iron homeostasis” Blood Cells Mol Diseases 29(3), 400-410. 2002.
 
[6]  Yang, C.S., Wang, X., Lu, G., and Picinich, S.C., “Cancer prevention by tea: animal studies, molecular mechanisms and human relevance” Nat Rev Cancer 9(6), 429-439. 2009.
 
[7]  Tsai, S.Y., Chang, Y., Chen, TL., and Chen, R.M., “Therapeutic concentrations of propofol protects mouse macrophages from nitric oxide-induced cell death and apoptosis” Can J Anaesth 49(5), 477-480. 2002.
 
[8]  Saddoughi, S.A., Song, P.F., and Ogretmen, B., “Roles of bioactive sphingolipids in cancer biology and therapeutics” Subcell Biochem 49, 413–440. 2008.
 
[9]  Hobbs, A.J., Higgs, A., and Moncada, S., “Inhibition of nitric oxide synthase as a potential therapeutic target” Annu Rev Pharmacol Toxicol 39, 191-220. 1999.
 
[10]  Marletta, M.A., “Nitric oxide synthase structure and mechanism” J Biol Chem 268(17), 12231-12234. 1993.
 
[11]  Chung, H.T., Pae, H.O., Choi, B.M., Billiar, T.R., and Kim, Y.M., “Nitric oxide as a bioregulator of apoptosis, Biochem” Biophys Res Commun 282(5), 1075-1079. 2001.
 
[12]  Xie, Q.W., Cho, H.J., Calaycay, J., Mumford, R.A., Swiderek, K.M., and Lee, T.D., “Cloning and characterization of inducible nitric oxide synthase from mouse macrophages” Science 256(5054), 225-228. 1992.
 
[13]  Lee, S.J., Lee, S.Y., Ha, H.J., Cha, S.H., Lee, S.K., and Hur, S.J., “Rutin Attenuates lipopolysaccharide-induced nitric oxide production in macrophage cells” J Food Nutr Res 3(3), 202-205. 2015.
 
[14]  Lee, S.J., Kim, Y.S., Hwang, J.W., Kim, E.K., Moon, S.H., Jeon, B.T., Jeon, Y.J., Kim, J.M., and Park, P.J., “Purification and characterization of a novel antioxidative peptide from duck skin by-products that protects liver against oxidative damage” Food Res Int 49, 285-295. 2012.
 
[15]  Bors, W., and Saran, M., “Radical scavenging by flavonoid antioxidants” Free Radic Res Commun 2(4-6), 289-294. 1987.
 
[16]  Fujimura, Y., Tachibana, H., and Yamada, K., “A tea catechin suppresses the expression of the high-affinity IgE receptor Fc ε RI in human basophilic KU812 cells” J Agric Food Chem 49(5), 2527-2531. 2001.
 
[17]  Chow, H.H., Cai, Y., Hakim, I.A., Crowell, J.A., Shahi, F., Brooks, C.A., Dorr, R.T., Hara, Y., and Alberts, D.S., “Pharmacokinetics and safety of green tea polyphenols after multiple-dose administration of epigallocatechin gallate and polyphenon E in healthy individuals” Clin Cancer Res 9(9), 3312-3319. 2003.
 
[18]  Wu, K.M., Ghantous, H., and Birnkrant, D.B., “Current regulatory toxicology perspectives on the development of herbal medicines to prescription drug products in United States” Food Chem Toxicol 46(8), 2606-2610. 2008.
 
[19]  Lee, S.J., Kim, E.K., Kim, Y.S., Hwang, J.W., Lee, K.H., Choi, D.K., Kang, H., Moon, S.H., Jeon, B.T., and Park, P.J., “Purification and characterization of a nitric oxide inhibitory peptide from Ruditapes philippinarum” Food Chem Toxicol 50(5), 1660-1666. 2012.
 
[20]  Kumazoe, M., Sugihara, K., Tsukamoto, S., Huang, Y., Tsurudome, Y., Takashi, S., Yumi, S., Naoki, U., Shuya, Y., Kim Y.H., Yamadam K., and Tachibana, H., “67-kDa laminin receptor increases cGMP to induce cancer-selective apoptosis” J Clin Invest 132(2), 787-799. 2013.