Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2015, 3(3), 196-201
DOI: 10.12691/jfnr-3-3-11
Open AccessArticle

Effects of Diallyl Disulfide in Elephant Garlic Extract on Breast Cancer Cell Apoptosis in Mitochondrial Pathway

Xin An1, Xiuhai Zhang2, Hongjun Yao1, Hongyang Li1 and Jianwu Ren1,

1Beijing Forestry University, College of Biological Sciences and Biotechnology, Beijing, P.R.China

2Beijing Academy of Agriculture and Forestry Sciences, Beijing, P.R.China

Pub. Date: March 24, 2015

Cite this paper:
Xin An, Xiuhai Zhang, Hongjun Yao, Hongyang Li and Jianwu Ren. Effects of Diallyl Disulfide in Elephant Garlic Extract on Breast Cancer Cell Apoptosis in Mitochondrial Pathway. Journal of Food and Nutrition Research. 2015; 3(3):196-201. doi: 10.12691/jfnr-3-3-11

Abstract

As a close relative of garlic, elephant garlic contains abundant allicin, which means the extract of elephant garlic might induce apoptosis in breast cancer cells. The elephant garlic was extracted in alcohol with Solvent - Microwave Extraction. The main component was analyzed by liquid chromatography. The MCF-7 (michigan cancer foundation -7) cells were cultured in vitro. The apoptosis rate was determined with Annexin V-FITC/PI staining based flow cytometry (FCM). Mitochondrial transmembrane potential variation in the cells was observed with JC-1 probe and fluorescence confocal microscopy. Caspase-3 was determined with fluorescence Western Blot. The main component of alcohol extract of elephant garlic was Diallyl Disulfide (DADS), and its relative content was 17.04 %. The early apoptosis of MCF-7 cells could be induced by the alcohol extract solutions and it involved the activation of mitochondrial pathway. The results indicated that the extract and DADS standards exerted pro-apoptotic effects on breast cancer cells in a dose-response manner. From the results of fluorescence confocal microscopy, it showed that the Red/Green fluorescence ratio of mitochondrial membrane potential in MCF-7 cells could be significantly decreased after inducing with DADS reference substances for 24 h. In addition, mitochondrial depolarization could be observed as adding the extract. The activated Caspase-3 was detected with Western Blot. It was demonstrated that the expression level of procaspase-3 had gone up with increased concentration of alcohol extract. The expression level was also increased with the treatment of DADS and the protein band was clear.

Keywords:
caspase-3 cell apoptosis Diallyl Disulfide (DADS) elephant garlic mitochondrial transmembrane potential

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References:

[1]  Nwachukwu, I.D., and Asawalam, E.F., “Evaluation of freshly prepared juice from garlic (Allium sativum L.) as a biopesticide against the maize weevil, Sitophilus zeamais (Motsch.) (Coleoptera: Curculionidae),” Journal of Plant Protection Research, 54 (2). 132-138. Jul.2014.
 
[2]  Nkrumah-Elie, Y.M., Reuben, J.S., Hudson, A., Taka, E., Badisa, R., Ardley, T., and et al, “Diallyl trisulfide as an inhibitor of benzo(a)pyrene-induced precancerous carcinogenesis in MCF-10A cells,” Food and Chemical Toxicology, 50 (7). 2524-2530. Apr.2012.
 
[3]  Sengupta, A., Ghosh, S., and Bhattacharjee, S., “Allium vegetables in cancer prevention: an overview,” Asian Pacific Journal of Cancer Prevention, 5 (3). 237-245. Jul.2004.
 
[4]  Cutler, R.R., and Wilson, P., “Antibacterial activity of a new, stable, aqueous extract of allicin against methicillin-resistant Staphylococcus aureus,” British Journal of Biomedical Science, 61 (2). 71-74. Mar.2004.
 
[5]  Park, S.Y., Cho, S.J., Kwon, H.C., Lee, K.R., Rhee, D.K., and Pyo, S., “Caspase-independent cell death by allicin in human epithelial carcinoma cells: involvement of PKA,” Cancer Letters, 224 (1). 123-132. Jun.2005.
 
[6]  Davis, S.R., “An overview of the antifungal properties of allicin and its breakdown products-the possibility of a safe and effective antifungal prophylactic,” Mycoses, 48 (2). 95-100. Mar.2005.
 
[7]  Lang, A., Lahav, M., Sakhnini, E., and et al, “Allicin inhibits spontaneous and TNF-alpha induced secretion of proinflammatory cytokines and chemokines from intestinal epithelial cells,” Clinical Nutrition, 23 (5). 1199-1208. Oct.2004.
 
[8]  Ghazanfari, T., Hassan, Z.M., and Khamesipour, A., “Enhancement of peritoneal macrophage phagocytic activity against Leishmania major by garlic (Allium Sativum) treatment,” Journal of Ethnopharmacology, 103 (3). 333-337. Feb.2006.
 
[9]  Galeone, C., Pelucchi, C., Levi, F., and et al, “Onion and garlic use and human cancer,” The American Journal of Clinical Nutrition, 84 (5). 1027-1032. Nov.2006.
 
[10]  Wang, F., Ogansawara, M.A., and Huang, P., “Small mitochondria targeting molecules as anti-cancer agents,” Molecular Aspects of Medicine, 31 (1). 75-92. Dec.2009.
 
[11]  Oommen, S., Anto, R.J., Srinivas, G., and Karunagaran, D., “Allicin (from garlic) induces caspase-mediated apoptosis in cancer cells,” European Journal of Pharmacology, 485 (1-3). 97-103. Feb.2004.
 
[12]  Kim, K., Rhee, D.K., and Pyo, S., “Differential apoptotic response of human breast cancer cells to allicin,” Faseb Journal, 25. 612.3. Mar.2011.
 
[13]  Park, B., Kim, K., Rhee, D.K., and et al, “The apoptotic effect of allicin in MCF-7 human breast cancer cells: role for ATF3,” Faseb Journal, 26. lb367. Mar.2012.
 
[14]  Pongsak, R., and Parichat,P., “Antimicrobial activity of elephant garlic oil against vibrio cholerae in vitro and in a food model,” Bioscience Biotechnology and Biochemistry, 73 (7). 1623-1627. Apr.2009.
 
[15]  Eric Block, John, A. D., and Robert, B.C., “Crushing Garlic and Slicing Onions: Detection of Sulfenic Acids and Other Reactive Organosulfur Intermediates from Garlic and Other Alliums using Direct Analysis in Real-Time Mass Spectrometry (DART-MS),” Phosphorus Sulfur and Silicon and the Related Elements, 186(5). 1085-1093. Jul.2011.
 
[16]  Qiao, A.X., Kang, L.M., Zhi, X.X., and Pan, J.G., “Optimum extraction of allicin from Allium sativum L. and its mathematical model,” Guangdong Agricultural Sciences, 20. 101-104. 2013.
 
[17]  He, L., Gu, Y., Liu, G.Z., Wang, Q.Z., Yan, S.L., and Li, J., “Optimization of ultrasound-assisted extraction of allicin using response surface methodology,” Food Science, 34 (6). 98-101. Mar.2013.
 
[18]  Pirak, T., Jangchud, A., and Jantawat, P., “Characterisation of physical, chemical and antimicrobial properties of allicin–chitosan complexes,” International Journal of Food Science and Technology, 47 (7). 1339-1347. Jul.2012.
 
[19]  Rouhani, M., Goliaei, B., Khodagholi, F., and Nikoofar, A., “Lithium increases radiosensitivity by abrogating DNA repair in breast cancer spheroid culture,” Archives of Iranian Medicine, 17 (5). 352- 360. May.2014.
 
[20]  Kumar, C.K., Lee, J., and Shivendra, V.S., “Critical role for reactive oxygen species in apoptosis induction and cell migration inhibition by diallyl trisulfide, a cancer chemopreventive component of garlic,” Breast Cancer Research and Treatment, 138 (1). 69-79. Feb.2013.
 
[21]  Sireesha, V.G., Kristie, G., Jennifer, L.D., and et al, “Identification of novel molecular regulators of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in breast cancer cells by RNAi screening,” Breast Cancer Research, 16 (2). R41. Apr.2014.