Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2015, 3(3), 162-168
DOI: 10.12691/jfnr-3-3-6
Open AccessArticle

Antimicrobial Effects of Camel Milk against Some Bacterial Pathogens

Magdy Hassan YASSIN1, 2, , Mahamed Mohamed Soliman3, 4, Salama Abd-Elhafez Mostafa1, 5 and Hussein Abdel-Maksoud Ali4

1Department of Medical Microbiology, Faculty of Applied Medical Sciences, Turabah, Taif University, Saudi Arabia

2Reproductive diseases Department, Animal Reproduction Research Institute, Al-Haram, Egypt

3Medical Laboratory Department, Faculty of Applied Medical Sciences, Turabah, Taif University, Saudi Arabia

4Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt

5Immunopharmacology Unit, Animal Reproduction Research Institute, Al-Haram, Egypt

Pub. Date: March 03, 2015

Cite this paper:
Magdy Hassan YASSIN, Mahamed Mohamed Soliman, Salama Abd-Elhafez Mostafa and Hussein Abdel-Maksoud Ali. Antimicrobial Effects of Camel Milk against Some Bacterial Pathogens. Journal of Food and Nutrition Research. 2015; 3(3):162-168. doi: 10.12691/jfnr-3-3-6


The present study was aimed to investigate the protective effects of camel milk against pathogenicity induced by Staphylococcus aureus (S. aureus) and E. coli in Wistar rats. Sixty healthy adult male Wistar rats were divided into six groups (10 per group). Group 1 served as a control without any treatment. Group 2 received camel milk for two consecutive weeks. Group 3 injected intraperitoneally (IP) by S. aureus in a doses of 2x109 CFU/ml per rat. Group 4 injected IP by E.coli in a dose of 5x1010 CFU/ml per rat. Group 5 supplemented with camel milk for two consecutive weeks and then injected IP by S.aureus (2x109 CFU/ml per rat). Group 6 supplemented with camel milk for two consecutive weeks and then injected IP by E.coli (5x1010 CFU/ml per rat). All animals were decapitated after 3 weeks, serum was extracted and liver, kidney and lung tissues were taken for pathogen isolation. The isolation rate and pathogenicity of S. aureus and E. coli was high in rats injected pathogens alone (group 3 and 4) compared to camel milk and pathogens administered rats (group 5 and 6). The isolation of S. aureus and E. coli was high in intestine, then lung, kidney and liver. Prior camel milk supplementation ameliorated the degree of pathogenicity induced by pathogens. Camel milk had synergistic action with ciprofloxacin against S. aureus and E. coli to reduce bacterial resistance and decrease the dose of antibiotics. Pathogens injection alone induced significant amelioration in liver and kidney functions and prior camel milk administration inhibited such changes. Moreover, oxidative stress represented by the increase in malondialdehyde levels in serum of pathogens injected rats was decreased by prior camel milk administration. In conclusion, camel milk has beneficial role as antibacterial food supplement against S.aureus and E.coli pathogenicity in Wistar rats.

camel milk supplementation antibacterial activity E. coli and S. aureus pathogenicity wistar rats

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[1]  El-Agamy, S.I., Ruppanner. R. and Ismail, A, “Antibacterial and antiviral activity of camel milk protective proteins”. Journal of Dairy Research., 59:169-175 . 1992.
[2]  Agrawal, R.P., Dogra, R., Mohta, N., Tiwari, R., Singhal, S. and Sultania, S, “Beneficial effect of camel milk in diabetic nephropathy”. Acta Biomedica., 80:131-134. 2009
[3]  EL-Fakharany, E.M., Nawal, A., Bakry, M.H., Lourdes, S., Nezar, A.R. and Elrashdy, M.R,” Anti-infectivity of camel polyclonal antibodies against hepatitis C virus in Huh7.5 hepatoma,” Virology Journal., 201: 1-9. 2012.
[4]  De Valdez, G.F., Bibi, W. and Bachmann, M.R,” Antimicrobial effect of the lactoperoxydase/thiocyanate/hydrogen peroxide (LP) system on the activity of thermophilic starter culture. Milchwissenschaft., 43: 350-352. 1988.
[5]  Kappeler, S.R., Ackermann, M., Farah, Z. and Puhan, Z,”Sequence analysis of camel (Camelus dromedarius) lactoferrin,” International Dairy Journal, 82 (9): 481-448. 1999.
[6]  Yagil, R,” Camel milk-a review,” International Journal of Animal Science, 2: 81-99. 1987.
[7]  Korhonen, H., Pihlanto, A,” Food-derived bioactive peptides opportunities for designing future foods,” Current Pharmaceutical Design 9: 1297-1308. 2001.
[8]  Omar, R.H., Eltinay, A.H,” Microbial quality of camel's raw milk in central southern region of united Arab Emirates,” Emir J Food Agric., 20 (1): 76-83. 2008.
[9]  Kappeler, S.R., Heuberger, C., Farah, Z. and Puhan, Z,” Expression of the peptidoglycan recognition protein, PGRP, in the lactating mammary gland,” Journal of Dairy Science, 87: 2660-2668. 2004.
[10]  Velioglu Ogünç, A., Manukyan, M., Cingi, A., Eksioglu-Demiralp, E., Ozdemir, A. A. and Süha, Y. A,” Dietary whey supplementation in experimental models of wound healing,” International Journal for Vitamin and Nutrition Research, 78 (2): 70-73. 2008.
[11]  Cimolai, N,” MRSA and the environment: implications for comprehensive control measures,” European Journal of Clinical Microbiology & Infectious Diseases, 27 (7): 481-493. 2008.
[12]  Welinder-Olsson, C., Kaijser, B,” Enterohemorrhagic Escherichia coli (EHEC),” Scandinavian Journal of Infectious Diseases, 37 (6-7): 405-416. 2005.
[13]  Cheesbrough, M,” Medical Laboratory Manual for Tropical Countries,” Microbiology, Linacre House, Jordan Hill Oxford p. 260. 2000.
[14]  Althnaian, T., Albokhadaim, I. and El-Bahr S.M,” Biochemical and histopathological study in rats intoxicated with carbontetrachloride and treated with camel milk,” Springer Plus, 2 (1): 57. 2013.
[15]  Cirioni, O., Giacometti, A., Ghiselli, R., Bergnach, C., Orlando, F., Silvestri, C., Mocchegiani, F., Licci, A., Skerlavaj, B., Rocchi, M., Saba, V. and Zanetti M,” Scalise GLL-37 protects rats against lethal sepsis caused by gram-negative bacteria,” Antimicrobial Agents Chemotherapy, 50 (5): 1672-1679. 2006.
[16]  Hari Prasad, O., Navya, A., Vasu, D. and Chiranjeevi, T,” Protective effects of Prosopis juliflora against Staphylococcus aureus induced hepatotoxicity in rats,” International Journal of Pharmacy & Biomedical Research, 2 (3): 172-178. 2011.
[17]  Osserman, E.F., Lawlor, D.P,” 1966. Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med 124: 921-952.
[18]  Wilson, P., Andrews, J.A., Charlesworth, R., Walesby, R., Singer, M., Farrell, D.J. and Robbins, M,” Linezolid resistance in clinical isolates of Staphylococcus aureus,” The Journal of Antimicrobial Chemotherapy, 51: 186-188. 2003.
[19]  Aarestrup, F.M., Jensen, N.E,” Development of penicillin resistance among Staphylococcus aureus isolated from bovine mastitis in Denmark and other countries,” Microbial & Drug Resistance, 4: 247-256. 1998.
[20]  Kris-Etherton, P. M., Hecker, K.D., Bonanome, A., Coval, S.M., Binkoski, A.E,” Hilpert, K.F., Griel, A.E. and Etherton, T.D,” Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer,” American Journal of Medicine, 113: 71-88. 2002.
[21]  Sboui, A., Khorchani, T., Agrebi, A., Djegham, M., Mokni, M. and Belhadj, O,” Antidiabetic effect of camel milk on alloxan-induced diabetic dogs. Afr J Microbiol Res., 6: 4023-4029. 2012.
[22]  Diarra, M.S., Peticlerc, D. and Lacasse, P,” Effect of lactoferrin in combination with penicillin on the morphology and the physiology of Staphylococcus aureus isolated from bovine mastitis,” Journal of Dairy sciences, 85 (5): 1141-1149. 2002.
[23]  El-Agamy, E.I., Ruppanner, R., Ismail, A., Champagne, C.P. and Assaf, R,” Purification and characterization of lactoferrin, lactoperoxidase, lysozyme and immunoglobulins from camel’s milk,” International Dairy Journal, 6: 129-145. 1996.
[24]  Benkerroum, N., Mekkaoui, M., Bennani, N. and Hidane, K,” Antimicrobial activity of camel’s milk against pathogenic strains of Escherichia coli and Listeria monocytogenes,” International Journal Dairy Technology, 57:39-43. 2008.
[25]  Abbas, S., Hifsa, A., Aalia, N. and Lubna, S,” Physico-chemical analysis and composition of camel milk,” International Research, 2 (2): 85-98. 2013.
[26]  Mwambete, K.D,” The in vitro antimicrobial activity of fruit and leaf crude extracts of Momordica charantia: a Tanzania medicinal plant,” African Health Science, 9 (1): 34-39. 2009.
[27]  Narmadha, G., Muneswararao, K., Rajesh, A. and Yenugu, S,” Characterization of a novel lysozyme-like 4 gene in the rat,” PLoS ONE, 6 (11): E27659-E27659. 2011.
[28]  Viswanathan, K., Thomas, A., Dharmaraj, R., Narayanasamy, M. and Rangarajan, B.N,” Assessment of antimicrobial activity of c-type lysozyme from Indian shrimp Fenneropenaeus Indicus,” Journal of Coastal Life Medicine, 2 (10): 757-761. 2014.
[29]  Shimada, T., Park, B.G., Wolf, A.J., Brikos, C., Goodridge, H.S. and Becker, C.A,” Staphylococcus aureus evades lysozyme-based peptidoglycan digestion that links phagocytosis, inflammasome activation, and IL-1β secretion,” Cell Host Microbe., 7 (1): 38-49. 2010.
[30]  Nikaido, H., Vaara, M,” Molecular basis of bacterial outer membrane permeability,” Microbiol Rev., 49:1-32. 1985.
[31]  Ibrahim, H.R., Matsuzaki, T. and Aoki, T,” Genetic evidence that antibacterial activity of lysozyme is independent of its catalytic function,” FEBS Letters, 506: 27-32. 2001.
[32]  Zdybicka-Barabas, A., Stączek, S., Mak, P., Skrzypiec, K., Mendyk, E., Cytryńska, M,” Synergistic action of Galleria mellonella apolipophorin III and lysozyme against Gram-negative bacteria. Biochim Biophys Acta, 1828 (6): 1449-1456. 2013.
[33]  Arnold, R.R., Brewer, M. and Gauthier, J.J,” Bactericidal activity of human lactoferrin: sensitivity of a variety of microorganisms,” Infection and Immunity, 28: 893-898. 1980.
[34]  Leitch, E.C., Willcox, M.D,” Synergic anti-staphylococcal properties of lactoferrin and lysozyme,” Journal of Medical Microbiology, 47: 837-842. 1998.
[35]  Rossi, P., Giansanti, F., Boffi, A., Ajello, M., Valenti, P., Chiancone, E. and Antonini, G,” Ca2+ binding to bovine lactoferrin enhances protein stability and influences the release of bacterial lipopolysaccharide,” Biochemistry and Cell Biology, 80: 41-48. 2002.
[36]  Valenti, P., Antonini, G,” Lactoferrin: an important host defense against microbial and viral attack,” Cellular and Molecular Life Sciences, 62: 2576-2587. 2005.
[37]  Melda, S., Ekrem, K., Murat, C., Hasan, O., Ilhami, G. and Ali A,” The prohibitive effect of lactoperoxidase system (LPS) on some pathogen fungi and bacteria,'' African Journal of Pharmacy and Pharmacology, 4: (9) 671-677. 2010.
[38]  Andersson, L.A., Bylkas, S.A. and Wilson, A.E,” Spectral analysis of lactoperoxidase. Evidence for a common heme in mammalian peroxidases,” Journal of Biological Chemistry, 271: 3406-3412. 1996.
[39]  Ueda, T., Sakamaki, K., Kuroki, T., Yano, I. and Nagata, S,” Molecular cloning and characterization of the chromosomal gene for human lactoperoxidase, Eurapian Journal of Biochemistry, 243: 32-41. 1997.
[40]  Cardoso, R.R., Ponte, M. and Leite V, '' Protective action of camel milk in mice inoculated with Salmonella enterica.'' Isr. Med. Assoc. J., 15 (1): 5-8. 2013.
[41]  Elagamy, E. I, '' Effect of heat treatment on camel milk proteins with respect to antimicrobial factors: a comparison with cows' and buffalo milk proteins.'' Food Chemistry, 68: 227-232. 2000.
[42]  Elagamy, E.I., Thapa, B.R., Walia, A,” Liver function tests and their interpretation,” Indian Journal of Pediatrics, 74: 663-671. 2007.
[43]  Dixon, J.B., Bhathal, P.S., Brien, P. E,” Nonalcoholic Fatty Liver Disease: Predictors of Nonalcoholic Steatohepatitis and Liver Fibrosis in the Severely Obese,” Gastroenterology, 121 (1): 91-100. 2001.
[44]  El-Demerdash, F.M., Yousef, M.I. and Zoheir, M.A,” 2005. Stannous chloride induces alterations in enzyme activities, lipid peroxidation and histopathology in male rabbit: antioxidant role of vitamin C,” Food and Chemical Toxicology, 43 (12): 1743-1752.
[45]  Yadav, N.P., Dixit, V.K,” 2003. Hepatoprotective activity of leaves of Kalanchoe pinnata Pers,” Journal of Ethnopharmacology, 86 (2-3): 197-202.
[46]  Roberts, R.A., Smith, R.A., Safe, S., Szabo, C., Tjalkens, R.B. and Robertson, F.M,” Toxicological and pathophysiological roles of reactive oxygen and nitrogen species. Toxicology, 276: 85-94. 2010.
[47]  Pandey, S., Singh, A., Kumar, P., Chaudhari, A. and Nareshkumar, G,” Probiotic Escherichia coli CFR 16 producing pyrroloquinoline quinone (PQQ) ameliorates 1,2-dimethylhydrazine-induced oxidative damage in colon and liver of rats,” Appl Biochem Biotechnol 173 (3) 775-786. 2014.
[48]  Exline, M.C., Crouser, E.D,” Mitochondrial mechanisms of sepsis induced organ failure,” Frontiers in Bioscience, 13: 5030-5041. 2008.
[49]  Ruggieri, A.J., Levy, R.J. and Deutschman, C.S,” 2010. Mitochondrial dysfunction and resuscitation in sepsis,” Crit. Care Clin., 26: 567-575.
[50]  Halliwell, B,” Lipid peroxidation, antioxidants and cardiovascular disease: how should e move forward? Cardiovascular Research, 47:410-418. 2000.
[51]  Victor, V.M., Espulgues, J.V., Hernandez-Mijares, A. and Rocha, M,” Oxidative stress and mitochondrial dysfunction in sepsis: a potential therapy with mitochondria-targeted antioxidants,” Infectious Disorders Drug Targets, 9: 376-89. 2009.
[52]  Galley H.F,” Oxidative stress and mitochondrial dysfunction in sepsis,” Britsh Journal of Anaesthesia, 107: 57-64. 2011.
[53]  Duarte, M.M., Rocha, J.B., Moresco, R.N., Duarte, T., Da Cruz, I.B., Loro, V.L. and Schetinger, M.R,” Association between ischemia-modified albumin, lipids and inflammation biomarkers in patients with hypercholesterolemia,” Clinical Biochemistry, 42 (7-8): 666-671. 2009.
[54]  Hopps, E., Noto, D., Caimi, G. and Averna, M.R,” A novel component of the metabolic syndrome: the oxidative stress. Nutr. Metab. Cardiovasc. Dis., 20: 72-77. 2010.
[55]  Hamelet, J., Demluth, K., Paul, J.L., Delabar, J.M. and Janel, N,” Hyperhomo-cysteinemia due to cystathionine beta synthase deficiency induces dysregulation of genes involved in hepatic lipid homeostasis in mice. Journal of Hepatolology, 46 (1): 151-159. 2007.
[56]  Kumar, S.A., Sudhahar, V. and Varalakshmi, P,” Protective role of eicosapentaenoate-lipoate (EPA-LA) derivative in combating oxidative hepatocellular injury in hypercholesterolemic atherogenesis. Atherosclerosis, 2006 189 (1): 115-122. 2006.