Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2015, 3(2), 114-120
DOI: 10.12691/jfnr-3-2-7
Open AccessArticle

Variation of Terpenoid Flavor Odorants in Bran of Some Black and White Rice Varieties Analyzed by GC×GC-MS

Watcharapong Chumpolsri1, Nataporn Wijit1, Pittayaporn Boontakham1, Piyarat Nimmanpipug1, Phumon Sookwong1, Suwaporn Luangkamin2 and Sugunya Wongpornchai1,

1Center of Excellence for Innovation in Chemistry and Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200 Thailand

2Department of Chemistry, Faculty of Science at Si Racha, Kasetsart University, Si Racha Campus, Chonburi 20230 Thailand

Pub. Date: February 12, 2015

Cite this paper:
Watcharapong Chumpolsri, Nataporn Wijit, Pittayaporn Boontakham, Piyarat Nimmanpipug, Phumon Sookwong, Suwaporn Luangkamin and Sugunya Wongpornchai. Variation of Terpenoid Flavor Odorants in Bran of Some Black and White Rice Varieties Analyzed by GC×GC-MS. Journal of Food and Nutrition Research. 2015; 3(2):114-120. doi: 10.12691/jfnr-3-2-7


Among the total of 146 volatiles extracted by SPME from the headspace of some black and white rice bran samples, twenty-eight terpenoid odorants were acculately identified. Most of these terpenoids possess good aroma character and are varied among three groups of Thai rice; black glutinous, black non-glutinous, and white non-glutinous. Of these three groups, black non-glutinous rice contains the greater number of these monoterpenoids. However, the content of the major terpenoid odorants, which are limonene, trans-β-ocimene, β-cymene, and linalool, is the highest in the bran of white Thai jasmine rice, Khao Dawk Mali 105 (KDML 105). An herbaceous odorant, myrcene, occurs in the bran of all black rice varieties but not in the bran of white rice. The flavor type of rice bran using these rice bran terpenoids was successfully classified using the chemometric principle component analysis method.

aroma terpenoids solid phase microextraction two-dimensional gas chromatography-mass spectrometry GC×GC-MS chemometrics PCA

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[1]  Buttery, R.G., Ling, L.C., and Juliano, B.O., “2-Acetyl-1-pyrroline: an important aroma component of cooked rice”. Chemistry and Industry, 1982. 23: 958-959.
[2]  Buttery, R.G., Turnbaugh, J.G., and Ling, L.C., “Contribution of volatiles to rice aroma”. Journal of Agricultural and Food Chemistry, 1988. 36(5): 1006-1009.
[3]  Buttery, R.G., Ling, L.C., Juliano, B.O., and Turnbaugh, J.G., “Cooked rice aroma and 2-acetyl-1-pyrroline”. Journal of Agricultural and Food Chemistry, 1983. 31: 823-828.
[4]  Wongpornchai, S., Sriseadka, T., and Choonvisase, S., “Identification and quantitation of the rice aroma compound, 2-acetyl-1-pyrroline, in bread flowers (Vallaris glabra Ktze)”. Journal of Agricultural and Food Chemistry, 2003. 51(2): 457-462.
[5]  Boo, H.O., Heo, B.G., and Gorinstein, S., “Analytical methods for enzyme and DPPH radical scavenging activities of natural pigments from some plants”. Food Analytical Methods, 2012. 5(6): 1354-1361.
[6]  Leardkamolkarn, V., Thongthep, W., Suttiarporn, P., Kongkachuichai, R., Wongpornchai, S., and Wanavijitr, A., “Chemopreventive properties of the bran extracted from a newly-developed Thai rice: The Riceberry”. Food Chemistry, 2011. 125(3): 978-985.
[7]  Adams, R.P., Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy. (essential oils). Vol. 3. 2001, Illinois USA: Allured Publishing Corporation. 9-456.
[8]  Mottram, R. The LRI & Odour Database, Research Group, School of Food Biosciences, University of Reading, UK. [cit. 1 April 2014]. 2005; Available from:
[9]  Goufo, P., Duan, M., Wongpornchai, S., and Tang, X., “Some factors affecting the concentration of the aroma compound 2-acetyl-1-pyrroline in two fragrant rice cultivars grown in South China”. Frontiers of Agriculture in China, 2010. 4: 1-9.
[10]  Yang, D.S., Lee, K.S., Jeong, O.Y., Kim, K.J., and Kays, S.J., “Characterization of volatile aroma compounds in cooked black rice”. Journal of Agricultural and Food Chemistry, 2008. 56(1): 235-240.
[11]  Yajima., I., Yanai, T., and Nakamura, M., “Volatile flavour components of cooked rice.” Agricultural and Biological Chemistry, 1978. 42: 1229-1233.
[12]  Yajima, I., Yanai, T., and Nakamura, M., “Volatile flavor components of cooked kaorimai (scented rice, O. sativa japonica)”. Journal of Agricultural and Biological Chemistry, 1979. 43: 2425-2430.
[13]  Ghelardini, C., Galeotti, N., Di Cesare Mannelli, L., Mazzanti, G., and Bartolini, A., “Local anaesthetic activity of beta-caryophyllene”. Il Farmaco, 2001. 56: 387-389.
[14]  Bullard, R.W. and Holguin, G., “Volatile components of unprocessed rice (Oryza sativa L.)”. Agricultural and Food Chemistry, 1977. 25(1): 99-103.
[15]  Sukhonthara, S., Theerakulkait, C., and Miyazawa, M., “Characterization of volatile aroma compounds from red and black rice bran”. Journal of Oleo Science, 2009. 58(3): 155-161.
[16]  Tsugita, T., Kurata, T., and Fujimaki, M., “Volatile components in the steam distillate of rice bran: identification of neutral and basic compounds”. Agricultural and Biological Chemistry, 1978. 42: 643-651.
[17]  Widjaja, R., Craske, J.D., and Wootton, M., “Comparative studies on volatile components of non-fragrant and fragrant rices”. Journal of the Science of Food and Agriculture, 1996. 70(2): 151-161.
[18]  Reverchon, E., “Fractional separation of SCF extracts from marjoram leaves: Mass transfer and optimization”. The Journal of Supercritical Fluids, 1992. 5(4): 256-261.
[19]  Bouwmeester, H.J., Gershenzon, J., Konings, M.C.J.M., and Croteau, R., “Biosynthesis of the monoterpenes limonene andcarvone in the fruit of caraway”. Plant Physiology, 1998. 117(3): 901-912.
[20]  Degenhardt, J., Köllner, T.G., and Gershenzon, J., “Monoterpene and sesquiterpene synthases and the origin of terpene skeletal diversity in plants”. Phytochemistry, 2009. 70(15-16): 1621-1637.
[21]  Dewick, P.M., Medicinal Natural Products A Biosynthetic Approach 3rd Edition. Vol. 3. 2009, West Sussex, United Kingdom: John Wiley & Sons Ltd. 193-206.