Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2015, 3(2), 77-87
DOI: 10.12691/jfnr-3-2-1
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Antihypercholesterolemic Effects of Mushroom, Chrysin, Curcumin and Omega-3 in Experimental Hypercholesterolemic Rats


1Medical Laboratory Department, Faculty of Applied Medical Sciences, Turabah, Taif University, Saudi Arabia

2Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Egypt

3Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt

4Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Egypt

5Department of Biochemistry, Animal Health Research Institute, Zagazig Branch, Egypt

Pub. Date: February 01, 2015

Cite this paper:
TAMER AHMED ISMAIL, MOHAMED MOHAMED SOLIMAN, MOHAMED ABDO NASSAN and DALIA IBRAHIM MOHAMED. Antihypercholesterolemic Effects of Mushroom, Chrysin, Curcumin and Omega-3 in Experimental Hypercholesterolemic Rats. Journal of Food and Nutrition Research. 2015; 3(2):77-87. doi: 10.12691/jfnr-3-2-1


Hypercholesterolemia and hypertriglyceridemia are major risk factors that accelerate the incidence of atherosclerosis and coronary artery diseases. Therefore, the present study was conducted to evaluate the hypolipidemic effect of widely known traditional medicinal herbs and omega-3 FA in experimental hypercholesterolemia induced by Triton WR-1339. Experimental hypercholesterolemic rats were administered mushroom, chrysin, curcumin and omega-3 for 2 weeks. Hypercholesterolemic rats showed an increase in serum levels of lipid profiles and hepatic enzymes. Hypercholesterolemic rats showed an increase in malondialdehyde (MDA) levels and a decrease in both serum levels and mRNA expression of catalase, superoxide dismutase (SOD) and glutathione reducatse. Moreover, hypercholesterolemic rats showed hepatic down regulation in the expression of genes related to fatty acids oxidation such as acyl CoA oxidase (ACO) and synthetase (ACS), together with carnityl palmityl transferase-1 (CPT-1) and peroxisome proliferator activator receptor-α (PPAR-α). Administration of mushroom, chrysin, curcumin and omega-3 to hypercholesterolemic rats for 2 weeks up-regulated significantly the down regulated genes. In contrast, expression of genes related to fatty acids biosynthesis and cholesterol metabolism were increased in hypercholesterolemic rats compared to control group. Herbal medications and omega-3 administration down regulated genes of fatty acids biosynthesis and cholesterol metabolism to normal expression. At cellular levels, hyperlipidemia induced fatty droplets accumulation, necrosis and presence of apoptotic hepatocytes together with leukocytic infiltration in necrotic area that are ameliorated and normalized after administration of herbs and omega-3. In conclusion, the current findings indicated that flavonoids (mushroom, chrysin, curcumin) and omega-3 possess antihypercholesterolemic effects at biochemical, molecular and histopathological levels and are useful in treatment of hypercholesterolemia with lower side effects compared with synthetic hypolipidemic drugs.

hypercholesterolemia curcumin mushroom chrysin omega-3 gene expression

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[1]  Formiguera X, Canton A. “Obesity: epidemiology and clinical aspects”, Best Pract Res Clin Gastroenterol., 18. 1125-1146. 2004.
[2]  Dhaliya, S.A., Surya, A.S., Dawn, V.T., Betty, C., Arun, K. and Sunil, C. “A review of hyperlipidemia and medicinal plants”, Int.J.A.PS. BMS., 2(4). 219-237. 2013.
[3]  Freedman, J. E. “High-fat diets and cardiovascular disease. Are nutritional supplements useful?”, Journal of the American College of Cardiology., 41 (10).1750-1752. 2003.
[4]  Duarte MM, Rocha JB, Moresco RN, Duarte T, Da Cruz IB, Loro VL, Schetinger MR. “Association between ischemia-modified albumin, lipids and inflammation biomarkers in patients with hypercholesterolemia”, Clinical Biochemistry, 42 (7-8). 666-671. 2009.
[5]  Hopps, E., Noto, D., Caimi, G. and Averna, M.R. “A novel component of the metabolic syndrome: the oxidative stress”, Nutr Metab Cardiovasc Dis., 20. 72-77. 2010.
[6]  Hamelet, J., Demluth, K., Paul, J.L., Delabar, J.M. and Janel, N. “Hyperhomo-cysteinemia due to cystathionine beta synthase deficiency induces dysregulation of genes involved in hepatic lipid homeostasis in mice”, J Hepatol., 46(1). 151-159. 2007.
[7]  Kumar, S.A., Sudhahar, V. and Varalakshmi, P. “Protective role of eicosapentaenoate-lipoate (EPA-LA) derivative in combating oxidative hepatocellular injury in hypercholesterolemic atherogenesis”, Atherosclerosis, 189(1). 115-122. 2006.
[8]  Eghdamian, B. and Ghose, K. “Mode of action and adverse effects of lipid lowering drugs”, Drugs Today (Barc), 34. 943-956. 1998.
[9]  Kumar, A.S., Mazumder, A. and Saravanan, V.S. “Antihyperlipidemic activity of Camellia sinensis leaves in triton wr-1339 induced Albino rats”, Pharmacogn Mag., 4.60-64. 2008.
[10]  Khanna, P. and Garcha, H.S. “Pleurotus mushroom - a source of food protein”, Mushrooms News letter Tropical, 4.9-14. 1984.
[11]  Jayakumar, T., Thomas, P.A., and Geraldine, P. “In-vitro antioxidant activities of an ethanolic extract of the oyster mushroom, Pleurotus ostreatus”, Inn Food Sci Emerg Tech., 10.228-234. 2009.
[12]  Bae, J., Sinha, J., Park, J.P., Song, C.H. and Yun, J.W. “Optimization of submerged culture conditions for exobiopolymer production by Paecilomyces Japanica”, J Microbiol Biotechnol., 10(4). 482-487. 2000.
[13]  Kanagasabapathy, G., Malek, S.N.A., Mahmood, A.A., Chua, K.H., Vikineswary, S. and Kuppusamy, U.R. “Beta-Glucan-Rich Extract from Pleurotus sajor-caju (Fr.) Singer Prevents Obesity and Oxidative Stress in C57BL/6J Mice Fed on a High-Fat Diet”, Evidence-Based Complementary and Alternative Medicine, 1-10. 2013.
[14]  Jegadeesh, R., Raaman, N., Hariprasath, L., Ramesh, V. and Srikumar, R. “Hypolipidemic Effect of Pleurotus djamor var. roseus in Experimentally Induced Hypercholesteromic Rats”, Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5(2). 581-588. 2014.
[15]  Phan, T., Yu, X.M., Kunnimalaiyaan, M. and Chen, H. “Antiproliferative effect of chrysin on anaplastic thyroid cancer”, J Surg Res., 170(1). 84-88. 2011.
[16]  Pushpavalli, G., Veeramani, C. and Pugalendi, K.V. “Influence of chrysin on hepatic marker enzymes and lipid profile against d-galactosamine-induced hepatotoxicity rats”, Food Chem Toxicol., 48(6). 1654-1659. 2010.
[17]  Sobocanec, S., Sverko, V., Balog, T., Saric, A., Rusak, G., Likic, S., Kusic, B., Katalinic, V., Radic, S. and Marotti, T. “Oxidant/antioxidant properties of Croatian native propolis”, J Agric Food Chem., 54(21). 8018-8026. 2006.
[18]  Aggarwal, B.B. “Targeting inflammation-induced obesity and metabolic diseases by curcumin and other nutraceuticals”, Annu Rev Nutr., 30. 173-199. 2010.
[19]  Meydani, M. and Hasan, S.T. “Dietary polyphenols and obesity. Nutrients, 2. 737. 2010.
[20]  Gao, S., Duan, X., Wang, X., Dong, D., Liu, D., Li, X., Sun, G. and Li, B. “Curcumin attenuates arsenic-induced hepatic injuries and oxidative stress in experimental mice through activation of Nrf2 pathway, promotion of arsenic methylation and urinary excretion”, Food Chem Toxicol., 59. 739-747. 2013.
[21]  Bradford, P.G. “Curcumin and Obesity”, International Union of Biochemistry and Molecular Biology Inc., 39(1). 78-87. 2013.
[22]  Bosch, J., Gerstein, H.C., Dagenais, G.R., Diaz, R., Dyal, L., Jung, H., Maggiono, A.P., Probstfield, J., Ramachandran, A., Riddle, M.C., Rydén, L.E. and Yusuf, S. “N-3 fatty acids and cardiovascular outcomes in patients with dysglycemia”, N Engl J Med., 367. 309-318. 2012.
[23]  Mozaffarian, D., Lemaitre, R.N., King, I.B., Song, X., Huang, H., Sacks, F.M., Rimm E.B., Wang, M. and Siscovick, D.S. “Plasma phospholipid long-chain omega-3 fatty acids and total and cause-specific mortality in older adults: a cohort study”, Ann Intern Med., 158. 515-525. 2013.
[24]  Lorente-Cebrián, S., Costa, A.G.V., Navas-Carretero, S., Zabala, M., Martínez, J.A and Moreno-Aliaga, M.J. “Role of omega-3 fatty acids in obesity, metabolic syndrome, and cardiovascular diseases: a review of the evidence”, J Physiol Biochem., 69.633-651. 2013.
[25]  Rossmeisl, M., Medrikova, D., van Schothorst, E.M., Pavlisova, J., Kuda, O., Hensler, M., Bardova, K., Flachs, P., Stankova, B., Vecka, M., Tvrzicka, E., Zak, A., Keijer, J. and Kopecky, J. “Omega-3 phospholipids from fish suppress hepatic steatosis by integrated inhibition of biosynthetic pathways in dietary obese mice”, Biochim Biophys Acta - Mol Cell Biol. Lipid, 1841. 267-278. 2014.
[26]  Venkadeswaran, K., Muralidharan, A.R., Annadurai, T., Ruban, V.V., Sundararajan, M., Anandhi, R., Thomas, P.A. and Geraldine, P. “Antihypercholesterolemic and Antioxidative Potential of an Extract of the Plant, Piper betle, and Its Active Constituent, Eugenol, in Triton WR-1339-Induced Hypercholesterolemia in Experimental Rats”, Evid Based Complement Alternat Med., 1-11. 2014.
[27]  Anandhi, R., Annadurai, T., Anitha, T.S., Muralidharan, A.R., Najmunnisha, K., Nachiappan, V., Thomas, P.A. and Geraldine, P. “Antihypercholesterolemic and antioxidative effects of an extract of the oyster mushroom, Pleurotus ostreatus, and its major constituent, chrysin, in Triton WR-1339-induced hypercholesterolemic rats”, J Physiol Biochem., 69(2).313-23. 2013.
[28]  Zarzecki, M.S., Araujo, S.M., Bortolotto, V.C., De Paula. M.T., Jesse, C.R. and Prigol, M. “Hypolipidemic action of chrysin on Triton WR-1339-inducedhyperlipidemia in female C57BL/6 mice”, Toxicology Reports, 1. 200-208. 2014.
[29]  Öner-İyidoğan Y, Koçak H, Seyidhanoğlu M, Gürdöl F, Gülçubuk A, Yildirim F, Çevik A, Uysal M. “Curcumin prevents liver fat accumulation and serum fetuin-A increase in rats fed a high-fat diet”, J Physiol Biochem. 2013, 69(4).677-86.
[30]  Flachs, P., Rossmeisl, M., Bryhn, M. and Kopecky. “Cellular and molecular effects of n−3 polyunsaturated fatty acids on adipose tissue biology and metabolism”, Clinical Science., 116. 1-16. 2009.
[31]  Bancroft, J.D. and Gamble, M. “Theory and Practice of Histological Techniques”, 6th ed. Churchill Livingstone Elsevier Philadelphia, 126-127. 2008.
[32]  Bertges, L.C., Souza Mourão, CAJr, J.B. and Cardoso, V.A.C. “Hyperlipidemiainduced by Triton WR1339 (Tyloxapol) in Wistar rats”, Rev. Bras. Cien.Med. Saúde., 1(1). 32-34. 2011.
[33]  Vijayaraj, P., Muthukumar, K., Sabarirajan, J. and Nachiappan, V. “Antihyperlipidemic activity of Cassia auriculata flowers in triton WR 1339 induced hyperlipidemic rats”, Exp Toxicol Pathol. 2011.
[34]  Thapa, B.R. and Walia, A. “Liver function tests and their interpretation”, Indian J Pediatr., 74. 663-671. 2007.
[35]  Dixon, J.B., Bhathal, P.S. O and Brien, P.E. “Nonalcoholic Fatty Liver Disease. Predictors of Nonalcoholic Steatohepatitis and Liver Fibrosis in the Severely Obese”, Gastroenterology, 121(1). 91-100. 2001.
[36]  Hassoun, E.A. and Stohs, S.J. “Comparative studies on oxidative stress as a mechanism for the fetotoxic of TCDD, endrin and lindane in C57BL/6J and DBA/2J mice”, Teratology, 51. 186. 1995.
[37]  El-Demerdash, F.M., Yousef, M.I. and Zoheir, M.A. “Stannous chloride induces alterations in enzyme activities, lipid peroxidation and histopathology in male rabbit: antioxidant role of vitamin C”, Food Chem Toxicol., 43(12). 1743-52. 2005.
[38]  Yadav, N.P. and Dixit, V.K. “Hepatoprotective activity of leaves of Kalanchoe pinnata Pers”, J Ethanopharmacol., 86, 197-202. 2003.
[39]  Kausar, S., Zaheer, Z., Saqib, M. and Zia, B. “The effect of Crataegus (Hawthorn) extract alone and in combination with simvastatin on serum lipid profile in hyperlipidemic albino rats”, Biomedica., 27. 140-147. 2011.
[40]  Wu, Y., Li, J., Wang, J., Si, Q., Zhang, J., Jiang, Y. and Chu, L. “Anti-atherogenic effects of centipede acidic protein in rats fed an atherogenic diet”, J Ethnopharmacol., 122(3). 509-516. 2009.
[41]  Benhizia, F., Lagrange, D., Malewiak, M.I.N. and Griglio, S. “In vivo regulation of hepatic lipase activity and mRNA levels by diets which modify cholesterol influx to the liver”, Biochim. Biophys. Acta Lipids lipid Metabol., 1211(2). 181-188. 1994.
[42]  Waterman, I.J. and Zammit, V.A. “Differential effects of fenofibrate or simvastatin treatment of rats on hepatic microsomal overt and latent diacylglycerol acyltransferase activities”, Diabetes, 51(6). 1708-1713. 2002.
[43]  Prasad, K. and Kalra, J. “Oxygen free radicals and hypercholesterolemic atherosclerosis: effect of vitamin E”, Am Heart J., 125.958-973. 1993.
[44]  Halliwell, B. “Lipid peroxidation, antioxidants and cardiovascular disease: how should we move forward?”, Cardiovasc Res., 47.410-418. 2000.
[45]  Oh, P.S., Lee, S.J. and Lim, K.T. “Hypolipidemic and antioxidative effects of the plant glycoprotein (36 kDa) from Rhus verniciflua stokes fruitin Triton WR-1339-induced hyperlipidemic mice”, Biosci Biotechnol Biochem., 70(2). 447-456. 2006.
[46]  Wissler, R.W. “Theories and new horizons in the pathogenesis of atherosclerosis and the mechanisms of clinical effects”, Arch Pathol Lab Med., 116.1281-1291. 1992.
[47]  Williams, C.A., Harborne, J.B., Newman, M., Greenham, J. and Eagles, J. “Chrysin and other leaf exudate flavonoids in the genus Pelargonium”, Phytochemistry, 46.1349-1353. 1997.
[48]  Ciftci, O., Ozdemir, I., Aydin, M. and Beytur, A. “Beneficial effects of chrysin on the reproductive system of adult male rats”, Andrologia, 44 (3). 181-186. 2012.
[49]  Rice-Evans, C.A. “Flavonoid antioxidants”, Curr Med Chem., 8 (7).797-807. 2001.
[50]  Palipoch, S., Punsawad, C., Koomhin, P. and Suwannalert, P. “Hepatoprotective effect of curcumin and alpha-tocopherol against cisplatin-induced oxidative stress”, BMC Complementary and Alternative Medicine, 14.111. 2014.
[51]  Naik, S.R., Thakare, V.N. and Patil, S.R. “Protective effect of curcumin on experimentally induced inflammation, hepatotoxicity and cardiotoxicity in rats. Evidence of its antioxidant property”, Exp Toxicol Pathol., 63(5).419-431. 2011.
[52]  Di Minno, M.N., Russolillo, A., Lupoli, R., Ambrosino, P., Di Minno, A. and Tarantino, G. “Omega-3 fatty acids for the treatment of nonalcoholic fatty liver disease”, World J Gastroenterol., 18(41).5839-5847. 2012.
[53]  Johnston, C. “Functional foods as modifiers of cardiovascular disease”, Am. J. Life style Med., 3 (1). 39S-43S. 2009.
[54]  Patil, U.K., Saraf, S. and Dixit, VK. “Hypolipidemic activity of seeds of Cassia tora Linn”, J Ethnopharmacol., 90.249-252. 2004.
[55]  Miller, N.E., La Ville, A. and Crook, D. “Direct evidence that reverse cholesterol transport is mediated by high-density lipoprotein in rabbit”, Nature, 314.109-111. 1985.
[56]  Zhao, J., Sun, X.B., Ye, F. and Tian, W.X. “Suppression of fatty acid synthase, differentiation and lipid accumulation in adipocytes by curcumin”, Mol Cell Biochem., 351. 19-28. 2011.
[57]  Ejaz, A., Wu, D., Kwan, P. and Meydani, M. “Curcumin inhibits adipogenesis in 3 T3-L1 adipocytes and angiogenesis and obesity in C57/BL mice”, J Nutr., 139. 919-25. 2009.
[58]  Shao, W., Yu, Z., Chiang, Y., Yang, Y., Chai, T., Foltz, W., Lu, H., Fantus, G. and Jin, T. “Curcumin Prevents High Fat Diet Induced Insulin Resistance and Obesity via Attenuating Lipogenesis in Liver and Inflammatory Pathway in Adipocytes”, PLoS ONE, 7(1). e28784. 2012.
[59]  Wang, S., Moustaid-Moussa, N., Chenb, L., Moc, H., Shastri, A., Su, R., Bapat, P., Kwun, I. and Shen, C.L. “Novel insights of dietary polyphenols and obesity”, Journal of Nutritional Biochemistry, 25.1-18. 2014.
[60]  Mozaffarian, D. and Wu, J.H. “(n-3) fatty acids and cardiovascular health. are effects of EPA and DHA shared or complementary? ”, J Nutr., 142(3).614S-625S. 2012.
[61]  Neschen, S., Moore, I., Regittnig, W., Yu, C.L., Wang, Y., Pypaert, M., Petersen, K.F. and Shulman, G.I. “Contrasting effects of fish oil and safflower oil on hepatic peroxisomal and tissue lipid content”, Am J Physiol Endocrinol Metab., 282. E395-E401. 2002.
[62]  Escher, P. and Wahli, W. “Peroxisome proliferator-activated receptors: insight into multiple cellular functions”, Mutat Res., 448. 121-138. 2000.
[63]  Brown, M.S. and Goldstein, J.L. “The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor”, Cell, 89. 331-340. 1977.
[64]  Matsumoto, T., Terai, S., Oishi, T., Kuwashiro, S., Fujisawa, K., Yamamoto, N., Fujita, Y., Hamamoto, Y., Furutani-Seiki, M., Nishina, H. and Sakaida, I. “Medaka as a model for human nonalcoholic steatohepatitis. Dis Model Mech., 3.431-440. 2010.
[65]  Schoonjans, K., Staels, B.and Auwerx, J. “Role of the peroxisome proliferator-activated receptor (PPAR) in mediating the effects of fibrates and fatty acids on gene expression”, J Lipid Res., 37. 907-25. 1996.
[66]  Harnafi, H., Caid, H.S., Bouanani, N.H., Aziz, M. and Amrani, S. “Hypolipemic activity of polyphenol-rich extracts from Ocimum basilicum in Triton WR-1339-induced hyperlipidemic mice”, Food Chem., 108. 205-212. 2008.
[67]  Iqbal, K., Khan, A. and Khattak, M.A. “Biological significance of ascorbic acid (Vit. C) in human health, a review”, Pakistan Journal of Nutrition, 3 (1). 5-13. 2004.
[68]  Popescu, L.A., Vîrgolici, B., Lixandru, D., Miricescu, D., Condruţ, E., Timnea, O., Ranetti, A.E., Militaru, M., Mohora, M. and Zăgrean, L. “Effect of diet and omega-3 fatty acids in NAFLD”, Rom J Morphol Embryol., 54(3).785-90. 2013.