Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2015, 3(1), 40-45
DOI: 10.12691/jfnr-3-1-7
Open AccessArticle

Effects of High Hydrostatic Pressure Extract of Korean Fresh Ginseng on Hepatic Lipid Accumulation and AMPK Activation in HepG2 Cells

Mak-Soon Lee1, Yoonjin Shin1, Sunyoon Jung1, Chong-Tai Kim2, In-Hwan Kim3 and Yangha Kim1,

1Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Republic of Korea

2Research Group of Convergence Technology, Korea Food Research Institute, Seongnam, Gyeonggi, Republic of Korea

3Department of Food and Nutrition, Korea University, Seoul, Republic of Korea

Pub. Date: January 22, 2015

Cite this paper:
Mak-Soon Lee, Yoonjin Shin, Sunyoon Jung, Chong-Tai Kim, In-Hwan Kim and Yangha Kim. Effects of High Hydrostatic Pressure Extract of Korean Fresh Ginseng on Hepatic Lipid Accumulation and AMPK Activation in HepG2 Cells. Journal of Food and Nutrition Research. 2015; 3(1):40-45. doi: 10.12691/jfnr-3-1-7


Ginseng is widely used as a medicinal herb and has demonstrated effects against liver diseases. The aim of this study is to investigate the hypolipidemic effects of the high hydrostatic pressure extract of Korean fresh ginseng (HEG) on hepatic lipid accumulation in HepG2 cells. The intracellular triglyceride and cholesterol contents were determined using enzymatic colorimetric methods. The mRNA levels of fatty acid synthase (FAS) and 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGCR) were assayed by quantitative real-time PCR. The activity of AMP-activated protein kinase (AMPK) was measured with an AMPK kinase assay kit. HEG significantly reduced hepatic triglyceride and cholesterol contents in HepG2 cells. Furthermore, HEG suppressed the expression of FAS, a key enzyme in fatty acid synthesis, and HMGCR, a rate-limiting enzyme in hepatic cholesterol synthesis. Additionally, HEG increased the activity of AMPK, a major regulator of lipid metabolism. These results suggest that HEG reduces hepatic lipid accumulation with inhibition of FAS and HMGCR expression and stimulation of AMPK activity in HepG2 cells. Consequently, HEG may be beneficial as a functional food ingredient to improve various hepatic diseases by reducing hepatic lipid accumulation.

korean fresh ginseng high hydrostastic pressure extract lipid accumulation FAS HMGCR AMPK

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[1]  Gaggini, M., Morelli, M., Buzzigoli, E., DeFronzo, R.A., Bugianesi, E. and Gastaldelli, A, “Non-Alcoholic Fatty Liver Disease (NAFLD) and Its Connection with Insulin Resistance, Dyslipidemia, Atherosclerosis and Coronary Heart Disease,” Nutrients, 5(5). 1544-1560. 2013.
[2]  Lee, J., Chung, D.S., Kang, J.H., and Yu, B.Y, “Comparison of Visceral Fat and Liver Fat as Risk Factors of Metabolic Syndrome,” J Korean Med Sci, 27(2), 184-189. Feb. 2012.
[3]  Bellentani, S., Scaglioni, F., Marino, M. and Bedogni, G, “Epidemiology of non-alcoholic fatty liver disease,” Dig Dis, 28. 155-161. 2010.
[4]  Lim, C.T., Kola, B. and Korbonits, M, “AMPK as a mediator of hormonal signalling,” J Mol Endocrinol, 44. 87-97. 2010.
[5]  Byeon, S.E., Lee, J., Kim, J.H., Yang, W.S., Kwak, Y.H., Kim, S.Y., Choung, E.S., Rhee, M.H., and Choi, J.Y, “Molecular mechanism of macrophage activation by red ginseng acidic polysaccharide from Korean red ginseng,” Mediators of Inflammation, 2012(2012). 1-7. 2012,
[6]  Choi, H., Kim T.H., Choi, T.Y., and Lee, M.S, “Ginseng for health care: a systematic review of randomized controlled trials in Korean literature,” PLoS ONE, 8(4). e59978. Apr. 2013.
[7]  Kim, J.H, “Cardiovascular Diseases and Panax ginseng: A Review on Molecular Mechanisms and Medical Applications,” J Ginseng Res, 36(1). 16-26. Jan. 2012.
[8]  Jung, J., Park, H.W., Hahn, Y., Hur, C.G., In, D., Chung, H.J., Liu, J. and Choi, D.W, “Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags,” Plant cell reports, 22. 224-230. 2003.
[9]  Yi, J.H., Kim, M.Y., Kim, Y.C., Jeong, W.S., Bae, D.W., Hur, J.M., Jun, M, “Change of ginsenoside composition in red ginseng processed with citric acid,” Food Sci Biotechnol, 19. 647-653. 2010.
[10]  Kim, S.O., Park, C.W., Moon, S., Lee, H.A., Kim, B., Lee, D.U., Lee, J.H. and Park, J, “Effects of high-hydrostatic pressure on ginsenoside concentrations in Korean red ginseng,” Food Sci Biotechnol, 16. 848-853. 2007.
[11]  Yuan, H.D., Kim, S.J., Quan, H.Y., Huang, B. and Chung, S.H, “Ginseng leaf extract prevents high fat diet-induced hyperglycemia and hyperlipidemia through AMPK activation,” J Ginseng Res, 34. 369-375. 2010.
[12]  Ji, W. and Gong, B, “Hypolipidemic effects and mechanisms of Panax notoginseng on lipid profile in hyperlipidemic rats,” J Ethnopharmacol, 113. 318-324. 2007.
[13]  Lee, H.S., Lee, H.J., Yu, H.J., Ju, D.W., Kim, Y., Kim, C.T., Kim, C.J., Cho, Y.J., Kim, N. and Choi, S.Y, “A comparison between high hydrostatic pressure extraction and heat extraction of ginsenosides from ginseng (Panax ginseng CA Meyer),” J Sci Food Agric, 91. 1466-1473. 2011.
[14]  Lee, S., Lee, M.S., Kim, C.T., Kim, I.H. and Kim, Y, “Ginsenoside Rg3 reduces lipid accumulation with AMP-activated protein kinase (AMPK) activation in HepG2 Cells,” Int J Mol Sci, 13. 5729-5739. 2012.
[15]  Rozen, S., Skaletsky, H, Primer3 on the WWW for general users and for biologist programmers In Bioinformatics methods and protocols: methods in molecular biology. Edited by Misener, S., and Krawetz, S, Humana Press, New Jersey, 1999, 365-386.
[16]  Livak, K.J. and Schmittgen, T.D, “Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method,” Methods, 25. 402-408. 2001.
[17]  Ou, T.T., Hsu, M.J., Chan, K.C., Huang, C.N., Ho, H.H. and Wang, C.J, “Mulberry extract inhibits oleic acid‐induced lipid accumulation via reduction of lipogenesis and promotion of hepatic lipid clearance,” J Sci Food Agric, 91.2740-2748. 2011.
[18]  Bhala, N., Jouness, R.I. and Bugianesi, E, “Epidemiology and natural history of patients with NAFLD,” Current Pharmaceutical Design, 19(29). 5169-5176. 2013.
[19]  Sohn, H., Lee, S. and Wee, J, “Flavor characteristics of Korean red ginseng,” J Ginseng Res, 24. 148-152. 2000.
[20]  Do Yeon Kim, J.S.P, Yuan, H.D. and Chung, S.H, “Fermented ginseng attenuates hepatic lipid accumulation and hyperglycemia through AMPK activation,” Food Sci Biotechnol, 18. 172-178. 2009.
[21]  Song, Y.B., An, Y.R., Kim, S.J., Park, H.W., Jung, J.W., Kyung, J.S., Hwang, S.Y. and Kim, Y.S, “Lipid metabolic effect of Korean red ginseng extract in mice fed on a high‐fat diet,” J Sci Food Agric, 92. 388-396. 2012.
[22]  Hillgartner, F.B., Salati, L.M. and Goodridge, A.G, “Physiological and molecular mechanisms involved in nutritional regulation of fatty acid synthesis,” Physiol Rev, 75. 47-76. 1995.
[23]  Goldstein, J.L. and Brown, M.S, “Regulation of the mevalonate pathway,” Nature, 343. 425-30. 1990.
[24]  Lee, Y.S., Cha, B.Y., Yamaguchi, K., Choi, S.S., Yonezawa, T., Teruya, T., Nagai, K. and Woo, J.T, “Effects of Korean white ginseng extracts on obesity in high-fat diet-induced obese mice,” Cytotechnology, 62. 367-376. 2012.
[25]  Muwalla, M.M, and Abuirmeileh, N.M, “Suppression of avian hepatic cholesterogenesis by dietary ginseng,” The Journal of nutritional biochemistry, 1(10). 518-521. Oct. 1990.
[26]  Hardie, D.G, “The AMP-activated protein kinase pathway–new players upstream and downstream,” J Cell Sci, 117. 5479-5487. 2004.
[27]  Quan, H.Y., Yuan, H.D., Jung, M.S., Ko, S.K., Park, Y.G. and Chung, S.H, “Ginsenoside Re lowers blood glucose and lipid levels via activation of AMP-activated protein kinase in HepG2 cells and high-fat diet fed mice,” Int J Mol Med, 29. 73. 2012.
[28]  Kim, D.Y., Yuan, H.D., Chung, I.K. and Chung, S.H, “Compound K, intestinal metabolite of ginsenoside, attenuates hepatic lipid accumulation via AMPK activation in human hepatoma cells,” J Agric Food Chem, 57. 1532-1537. 2009.