Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2014, 2(12), 1007-1014
DOI: 10.12691/jfnr-2-12-23
Open AccessArticle

Purification, Physicochemical Characterization, and Bioactivities of Polysaccharides from Puerh Tea

Limin Mao1, 2, Shuhong Shao1, Shili Sun3, Yuefei Wang1, 2, Ping Xu1 and Liewei Cai1, 4,

1Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, China

2Zhejiang Tea Science Society, Hangzhou, China

3Drinkable Plants Institute (Tea Research Center)/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization, Guangzhou, China

4Zhangzhou College of Science & Technology, Zhangzhou, China

Pub. Date: December 02, 2014

Cite this paper:
Limin Mao, Shuhong Shao, Shili Sun, Yuefei Wang, Ping Xu and Liewei Cai. Purification, Physicochemical Characterization, and Bioactivities of Polysaccharides from Puerh Tea. Journal of Food and Nutrition Research. 2014; 2(12):1007-1014. doi: 10.12691/jfnr-2-12-23

Abstract

Two fractions of polysaccharides, named PTPS-1 and PTPS-2, was extracted and purified from puerh tea by Sephacryl S-300 column chromatography. The physicochemical properties of these two polysaccharides were investigated by high-performance liquid chromatography (HPLC), Fourier Transform IR spectra, scanning electron microscope (SEM) and atomic force microscope (AFM). Analysis of chemical compositions (protein, neutral sugars, uronic acid and monosaccharide composition) suggested that they were both kinds of acid heteropolysaccharides bound with protein, and contained seven monosaccharides with different molar ratio. Meanwhile, evaluation of antioxidant activities by in vitro assays of DPPH, ABTS and FRAP showed that PTPS-1 demonstrated stronger antioxidant ability than PTPS-2. Similarly, PTPS-1 exhibited remarkable inhibitory potential on α-glycosidase in vitro, significantly stronger than that of PTPS-2 and acarbose. Moreover, the results from animal test indicated PTPS-1 possessed significant inhibition on postprandial hyperglycemia in diabetic mice compared with the model group.

Keywords:
puerh tea polysaccharides purification antioxidant activity α-glycosidase inhibition physicochemical characterization

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