Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2014, 2(12), 1000-1006
DOI: 10.12691/jfnr-2-12-22
Open AccessArticle

The Effect of Fraction 5 of Theabrownin from Pu-erh Tea on 3T3-L1 Preadipocyte Proliferation and Differentiation

Xinhe Yang1, 2, Zhonghua Liu1, 3, , Jianan Huang2, 4, Caiqin Qin5, Qingli Mao1, 2 and Qin Li2, 4

1School of Life Science and Technology, Hubei Engineering University, Xiaogan, China

2National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha, China

3National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha, China;Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, China

4Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, China

5College of Chemistry and Materials Science, Hubei Engineering University, Xiaogan, China

Pub. Date: November 30, 2014

Cite this paper:
Xinhe Yang, Zhonghua Liu, Jianan Huang, Caiqin Qin, Qingli Mao and Qin Li. The Effect of Fraction 5 of Theabrownin from Pu-erh Tea on 3T3-L1 Preadipocyte Proliferation and Differentiation. Journal of Food and Nutrition Research. 2014; 2(12):1000-1006. doi: 10.12691/jfnr-2-12-22


Pu-erh tea, a well-known tea from ancient times, was originally produced in the Yunnan province of China. Theabrownin of pu-erh tea was successfully separated into six fractions by using a sephadex LH-20 column. A previous study showed that the inhibition of α-glycosidase and pancreatic lipase activities was enriched in theabrownin fraction 5 (F5). Here, the effects of F5 on the proliferation and differentiation of 3T3-L1 preadipocytes were investigated. Results showed that F5 suppressed 3T3-L1 preadipocyte proliferation and during 3T3-L1 preadipocyte differentiation, F5 dose-dependently promoted lipid accumulation and significantly decreased the expression of PPARγ2, PTP1B mRNA and protein and Glut4 protein as well as increased the expression of Glut4 mRNA. The findings suggested that F5 act to maintain a reasonable lipid metabolism balance by regulating the mRNA and protein expression of PPARγ2, and allow the activation of insulin receptor proteins to promote Glut4 translocation to the cell membrane and glucose intake by decreasing PTP1B protein expression. Together, these activities may improve Insulin resistance (IR) and prevent metabolic syndrome, which is important for establishing a basis for the development of F5 into a functional food.

pu-erh tea theabrownin fraction 5 3T3-L1 preadipocyte PPARγ2 PTP1BGlut4

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