Effect of Geographical Region and Harvesting Date on Antioxidant Activity, Phenolic and Flavonoid Content of Olive Leaves

Fuad Al-Rimawi^1, , Imad Odeh¹, Abdallah Bisher¹, Jehad Abbadi² and Mohammad Qabbajeh¹

¹Chemistry Department, Faculty of Science and Technology, Al-Quds University, Jerusalem, Palestine

²Biology Department, Faculty of Science and Technology, Al-Quds University, Jerusalem, Palestine

Cite this paper:
Fuad Al-Rimawi, Imad Odeh, Abdallah Bisher, Jehad Abbadi and Mohammad Qabbajeh. Effect of Geographical Region and Harvesting Date on Antioxidant Activity, Phenolic and Flavonoid Content of Olive Leaves. Journal of Food and Nutrition Research. 2014; 2(12):925-930. doi: 10.12691/jfnr-2-12-11

Abstract

The effect of geographical region and harvesting date (seasonal change) on antioxidant activities (AA), total phenolic content (TPC) and total flavonoid content (TFC) of olive leaves obtained from different geographical regions of Palestine (north, middle, and south) at different maturation stages (June 2013, October 2013, and January 2014) was investigated in this study. Results revealed that both geographical region and maturation stage affect AA, TPC, and TFC of the olive leaves. Highest AA, TPC, and TFC were obtained for samples collected in June. TPC was found to be highest in north and lowest in south, while the highest AA, and TFC contents were alternating between north, middle, and south. During different maturation stages, TPC, TFC, and AA varied between 21.56 - 47.52 mg (GAE), 19.3 - 32.6 mg catechin equivalents, 318.53 – 1106.43 µmol FRAP equivalents per gram of dry olive leaves, respectively.

Keywords:
Olive leaves antioxidant activity phenolic content flavonoid content harvesting date geographical region

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References:

[1]	El, S.N., and Karakaya, S. “Olive tree (Olea europaea) leaves: potential beneficial effects on human health”, Nutr Rev. 67, 632-638. 2009.
[2]	Tabera, J., Guinda, A., Ruiz-Rodriguez, A., Senorans, F.J., and Ibanez, E. “Countercurrent Supercritical Fluid Extraction and Fractionation of High-Added-Value Compounds from a Hexane Extract of olive Leaves”. J Agric Food Chem. 52. 4774-4779. 2004.
[3]	Fito, M., De La Torre, R., and Covas, M.I. “Olive oil and oxidative stress”. Mol. Nutr. Food Res. 51. 1215-1224. 2007.
[4]	Khayyal, M.T., el-Ghazaly, M.A., Abdallah, D.M., Nassar, N.N., Okpanyi, S.N., and Kreuter, M.H. “Blood pressure lowering effect of an olive leaf extract (Olea europaea ) in L-NAME induced hypertension in rats”. Arzneimittelforschung. 52. 797-802. 2002.
[5]	Bisignano, G., Tomaino, A., Lo-Cascio, R., Crisafi, G., Uccella, N., and Saija, A. “On the in-vitro antimicrobial activity of oleuropein and hydroxytyrosol”. J Pharm Pharmacol. 51. 971-974. 1999.
[6]	Micol, V., Caturla, N., Perez-Fons, L., Mas, V., Perez, L., and Estepa, A. “The olive leaf extract exhibits antiviral activity against viral haemorrhagic septicaemia rhabdovirus (VHSV)”. Antiviral Res. 66. 129-136. 2005.
[7]	Ryan, D., Antolovich, M., Prenzler, P., Robards, K., and Lavee, S. “Biotransformations of phenolic compounds in Olea europaea L. Scientia Horticulturae”. 92. 147-176. 2002.
[8]	Briante, R., Patumi, M., Limongelli, S., Febbraio, F., Vaccaro, C., Di Salle, A., La Cara, F., and Nucci, R. “Changes in phenolic and enzymatic activities content during fruit ripening in two Italian cultivars of Olea europaea L.” Plant Science. 162. 791-798. 2002.
[9]	Hajimahmoodi, M., Sadeghi, N., Jannat, B., Oveisi, M.R., Madani, S., Kiayi, M., Akrami, M.R., Ranjbar, A.M. “Antioxidant Activity, Reducing Power and Total Phenolic Content of Iranian Olive Cultivar”. Journal of biological sciences. 8. 779-783. 2008.
[10]	Kostas Kiritsakis, M.G., Kontominas, C., Kontogiorgis, D., Hadjipavlou-Litina, A., Moustakas, A., and Kiritsakis, K. “Composition and Antioxidant Activity of Olive Leaf Extracts from Greek Olive Cultivars”. Journal of the American Oil Chemists' Society. 87. 369-376. 2010.
[11]	Ben Salah, M., Abdelmelek, H., and Abderraba, M. “Study of Phenolic Composition and Biological Activities Assessment of Olive Leaves from different Varieties Grown in Tunisia”. Medicinal chemistry. 2. 107-111. 2012.
[12]	Góis Diogo, J.S. “Valorization of wild olives (Olea europaea var. sylvestris) as potential source of functional ingredients”, universidade de lisboa, PhD dissertation, 2013.
[13]	Abdel-Sattar, E.A., Abdallah, H.M., Khedr, A., and Abdel-Naim, A.B. “Chemical and Biological Assessment of African Olive Leaf Extract”. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 3. 155-172. 2012.
[14]	Singleton, V. L., Joseph, A., and Rossi Jr. “Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents”. Am. J. Enol. Vitic. 16, 144-158. 1965.
[15]	Kim, D.O., Jeong, S.W., Lee, C.Y. “Antioxidant capacity of phenolic phytochemicals from various cultivars of palms”. Food Chemistry. 81. 321-326. 2003.
[16]	Benzie, I.F., and Strain, J.J. “Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration”. Methods in Enzymology. 299. 15-27. 1999.
[17]	Helen, L. “Extraction of antioxidant compounds from olive (olea uropaea) leaf”, Massey University, albanya, New Zeealand, master thesis. 2011.
[18]	Abaza, L., Ben Youssef, N., Manai, H., Haddada, F.M., Methenni, K., and Zarrouk, M. “Chétoui olive leaf extracts: influence of the solvent type on phenolics and antioxidant activities”. grasas y aceites. 62. 96-104. 2011.
[19]	Bhoyar, M.S., Mishra, G.P., Naik, P.K., and Srivastava, R.B. “Estimation of antioxidant activity and total phenolics among natural populations of Caper (Capparis spinosa) leaves collected from cold arid desert of trans-Himalayas”. AJCS. 5. 912-919. 2011.