Study on Molecular Tracking and Bioactivity of Glucocerebroside Isolated from Radish (Raphanus sativus L.)

Guifeng Li^1, , Guixin Li², Haixia Fan¹, Mingdi Xia³, Jiangsheng Mao⁴, Yebing Chen⁴ and Changying Guo⁴

¹Shandong Lvyuan Agricultural Science and Technology Research Institute, Weifang, China

²Affiliated Hospital of Weifang Medical College, Weifang, China

³Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, China

⁴Institute of Agricultural Quality Standards and Testing Technology Research, Shandong academy of agricultural sciences, Jinan, China

Cite this paper:
Guifeng Li, Guixin Li, Haixia Fan, Mingdi Xia, Jiangsheng Mao, Yebing Chen and Changying Guo. Study on Molecular Tracking and Bioactivity of Glucocerebroside Isolated from Radish (Raphanus sativus L.). Journal of Food and Nutrition Research. 2014; 2(12):914-917. doi: 10.12691/jfnr-2-12-9

Abstract

The cruciferous herb Weixian radish (Raphanus sativus L.), also known as Lai Fu, is a popular type of edible vegetable. The present paper describes the molecular tracking and bioactivity of glucocerebroside isolated from Weixian radish for the first time. Methanol extraction was carried out by using silica gel, Sephadex LH-20 and RP-C18 column chromatography. Based on their structures, the two compounds were determined to be 1-O-(β-D-glucopyranosyl)-(2S, 3S, 4R, 8E)-2-[(2'R)-2'-hydroxyl-tetracos-15'-enoyl amino]-8-octa-decene- 1, 3, 4-triol (glucocerebroside). The glucocerebroside could inhibit the growth of BEL-7402 cancer cells and induce apoptosis in these cells.

Keywords:
Raphanus sativus L. glucocerebroside molecular tracking bioactivity

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