Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2014, 2(12), 876-879
DOI: 10.12691/jfnr-2-12-3
Open AccessArticle

Chickpea Seeds Ferritin as a Potential Source in the Treatment of Iron Deficiency Anemia

Yun Huang1, 2, , Min Xin1, Qian Li1, Xiaohong Luo2, Xiaowen Wang3, Xianwei Zou1, Xuan Liu1, Benke Chen2 and Jintian Tang1

1Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing, China

2Beijing Longcredit Essence Biotechnology Co. Ltd, Beijing, China

3Institute of neurological disorders, Yuquan hospital, Tsinghua University, Beijing, China

Pub. Date: November 05, 2014

Cite this paper:
Yun Huang, Min Xin, Qian Li, Xiaohong Luo, Xiaowen Wang, Xianwei Zou, Xuan Liu, Benke Chen and Jintian Tang. Chickpea Seeds Ferritin as a Potential Source in the Treatment of Iron Deficiency Anemia. Journal of Food and Nutrition Research. 2014; 2(12):876-879. doi: 10.12691/jfnr-2-12-3


Iron deficiency anemia (IDA) is one of the most common nutritional problems that encountered all over the world. This study focused on the effects of chickpea seeds ferritin (CSF) as aniron supplement on IDA in rats. Six groups of female Wistar rats (n = 8) were used, which contain (1) control group; (2) IDA model group; (3) FeSO4 positive control group (dosages of iron is 3 mg/(kg•d)); (4) CSF high-dose group (dosage of iron is 3 mg/(kg•d); (5) CSF medium-dose group (dosage of iron is 1.5 mg/(kg•d)); and (6) CSF low-dose group (dosage of iron is 0.75 mg/(kg•d)). After 2 weeks, the hemoglobin (Hb) concentration value, serum iron (SI) stores, body weights, blood parameters and tissue weights of anemic rats were measured, respectively. It showed that the Hb concentration value and SI stores were significantly increased in the iron supplement groups (CSF, FeSO4) compared with the IDA model group (P< 0.05). Additionally, the changes of red blood cell (RBC) number and HCT (Hematocrit) level in CSF groups were significantly greater than the IDA model group at the end of the experiment. All in all, compared with FeSO4 group, a higher bioavailability of iron and fewer side effects were observed in the CSF groups. Thus, the present study indicated that CSF was an effective source of iron supplement for the IDA model rats and could be developed as a functional product to overcome the malnutrition-related iron deficiency.

iron deficiency anemia chickpea seeds ferritin hemoglobin serum iron iron supplement

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[1]  Haas, J.D. and Brownlie,T, “Iron deficiency and reduced work capacity: a critical review of the research to determine a causal relationship”. The Journal of nutrition, 131 (2): 676S-690S. 2001.
[2]  Grantham-McGregor, S.andAni, C,“A review of studies on the effect of iron deficiency on cognitive development in children”. The Journal of nutrition, 131 (2): 649S-668S. 2001.
[3]  Sachdev, H.P.S. and Gera, T, “Nestel P “Effect of iron supplementation on mental and motor development in children: systematic review of randomised controlled trials”. Public health nutrition, 8 (02): 117-132. 2005.
[4]  Levy, A., Fraser, D., Katz, M., Mazor, M. andSheiner,E, “Maternal anemia during pregnancy is an independent risk factor for low birthweight and preterm delivery”. European Journal of Obstetrics & Gynecology and Reproductive Biology, 122 (2): 182-186. 2005.
[5]  Ahluwalia, N., Sun, J., Krause, D., Mastro, A. and Handte,G, “Immune function is impaired in iron-deficient, homebound, older women”. The American journal of clinical nutrition, 79 (3): 516-521. 2004.
[6]  Moench-Pfanner, R., de Pee S, Bloem, M.W., Foote, D.,Kosen, S. and Webb, P, “Food-for-work programs in Indonesia had a limited effect on anemia”. The Journal of nutrition, 135 (6): 1423-1429. 2005.
[7]  Layrisse, M., García-Casal, M.N., Solano, L., Barón, M.A., Arguello, F., Llovera, D. andTropper, E, “Iron bioavailability in humans from breakfasts enriched with iron bis-glycine chelate, phytates and polyphenols”. The Journal of nutrition, 130(9): 2195-2199. 2000.
[8]  Layrisse, M., García-Casal, M.N., Solano, L, Barón, M.A., Arguello, F, Llovera, D. and Tropper, E, “Milk inhibits and ascorbic acid favors ferrous bis-glycine chelate bioavailability in humans”. The Journal of nutrition, 127 (7): 1407-1411. 2000.
[9]  Pineda, O., Wayne Ashmead, H.D., Perez, J.M. and Lemus, C.P, “Effectiveness of iron amino acid chelate on the treatment of iron deficiency anemia in adolescents”. Journal of Applied Nutrition 46 (1): 2-13. 1994.
[10]  Pizarro, F., Olivares, M., Hertrampf, E., Mazariegos, D.I., Arredondo, M., Letelier, A. andGidi, V, “Iron bis-glycine chelate competes for the nonheme-iron absorption pathway”. The American journal of clinical nutrition, 76 (3): 577-581. 2002.
[11]  Kumar, G.B.S., Srinivas, L. and Ganapathi, T.R, “Iron fortification of banana by the expression of soybean ferritin”. Biological trace element research, 142 (2): 232-241. 2011.
[12]  Tuma, R.B., Yuyama, L.K.O., Aguiar, J.P.L. and Marques, H.O, “Impacto da farinha de mandiocafortificada com ferroaminoácidoquelato no nível de hemoglobina de pré-escolares”. Rev. nutr, 16 (1): 29-39. 2003.
[13]  Yun, S., Zhang, T., Li, M., Chen, B. and Zhao, G, “Proanthocyanidins inhibit iron absorption from soybean (Glycine max) seed ferritin in rats with iron deficiency anemia”. Plant foods for human nutrition, 66 (3): 212-217.2011.
[14]  Wang, F.R., Xie, Z. G., Ye, X.Q., Deng, S.G., Hu, Y.Q., Guo, X. and Chen, S.G, “Effectiveness of treatment of iron deficiency anemia in rats with squid ink melanin–Fe”. Food & function, 5(1):123-128.2014.
[15]  Damodar, S., Raghunath, S.T., Murthy, S., Jayanthi, K.J. and Latha, B.S,“Low Hemoglobin Density as a Measure of Iron Status”. Indian Journal of Hematology and Blood Transfusio, 29 (2): 75-76. 2013.
[16]  Borel, M.J., Smith, S.H., Brigham, D.E. and Beard, J.L, “The impact of varying degrees of iron nutriture on several functional consequences of iron deficiency in rats”. The Journal of nutrition, 121 (5): 729-736. 1991.
[17]  Strube, Y.N.J., Beard, J.L. and Ross, A.C, “Iron deficiency and marginal vitamin A deficiency affect growth, hematological indices and the regulation of iron metabolism genes in rats”. The Journal of nutrition, 132 (12): 3607-3615.2002.
[18]  Rossi, M.A., Carillo, S.V. and Oliveira, J.S.M, “The effect of iron deficiency anemia in the rat on catecholamine levels and heart morphology”. Cardiovascular research, 15 (6): 313-319. 1981.