Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2014, 2(11), 781-785
DOI: 10.12691/jfnr-2-11-4
Open AccessArticle

HPLC/PDA–ESI/MS Identification of Phenolic Acids, Flavonol Glycosides and Antioxidant Potential in Blueberry, Blackberry, Raspberries and Cranberries

ZORIŢA DIACONEASA1, , RANGA FLORICA1, DUMITRIŢA RUGINĂ2, CUIBUS LUCIAN1 and CARMEN SOCACIU1,

1Faculty of Food Science and Technology, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Romania, Calea Mănăştur 3-5, Cluj-Napoca, Romania

2Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Romania, Calea Mănăştur 3-5, Cluj-Napoca, Romania

Pub. Date: October 22, 2014

Cite this paper:
ZORIŢA DIACONEASA, RANGA FLORICA, DUMITRIŢA RUGINĂ, CUIBUS LUCIAN and CARMEN SOCACIU. HPLC/PDA–ESI/MS Identification of Phenolic Acids, Flavonol Glycosides and Antioxidant Potential in Blueberry, Blackberry, Raspberries and Cranberries. Journal of Food and Nutrition Research. 2014; 2(11):781-785. doi: 10.12691/jfnr-2-11-4

Abstract

Berry fruits are known to be a rich source of natural compounds which provide them many health benefits. The most common compounds that occur in berries are vitamins, flavonoids, anthocyanins and phenolic acids. The objective of this work was to identify and compare the phenolic acids, flavonol glycosides content and antioxidant potential in blueberry, blackberry, raspberries and cranberries. Berries methanolic extracts were analyzed by HPLC/PDA–ESI/MS. Antioxidant activity was determinate using FRAP assay. Antioxidant activity of analyzed berries varied considerably. Blueberry extract demonstrated the highest ferric reducing antioxidant potential (48.3 μM Fe2/g) while lower values were obtained for cranberries extract (19.6 μM Fe2/g). Berries extracts were characterized by the presence of 16 compounds in different ratios. Blueberry extract was characterized by the presence of a large amount of phenolic acids such as chlorogenic and caffeic acid while raspberries extract was found to have high amount of ellagic acid. All analyzed berries contain higher levels of bioactive compounds which are responsible for their antioxidant potential. Based on quantitative analysis of phenolics these fruits can be highly recommended for daily consumption.

Keywords:
berries phenolic acids flavonol glycosides antioxidant activity HPLC-ESI/MS

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References:

[1]  Yun, J.M., et al., Delphinidin, an anthocyanidin in pigmented fruits and vegetables, induces apoptosis and cell cycle arrest in human colon cancer HCT116 cells. Mol Carcinog, 2009. 48 (3): p. 260-70.
 
[2]  Maatta-Riihinen, K.R., A. Kamal-Eldin, and A.R. Torronen, Identification and quantification of phenolic compounds in berries of Fragaria and Rubus species (family Rosaceae). J Agric Food Chem, 2004. 52 (20): p. 6178-87.
 
[3]  Kao, E.S., et al., Anti-inflammatory potential of flavonoid contents from dried fruit of Crataegus pinnatifida in vitro and in vivo. J Agric Food Chem, 2005. 53 (2): p. 430-6.
 
[4]  Hertog, M.G.L., P.C.H. Hollman, and B. van de Putte, Content of potentially anticarcinogenic flavonoids of tea infusions, wines, and fruit juices. Journal of Agricultural and Food Chemistry, 1993. 41 (8): p. 1242-1246.
 
[5]  Jing, P. and M.M. Giusti, Contribution of Berry Anthocyanins to Their Chemopreventive Properties, in Berries and Cancer Prevention, N.P. Seeram and G.D. Stoner, Editors. 2011, Springer New York. p. 3-40.
 
[6]  Routray, W. and V. Orsat, Blueberries and Their Anthocyanins: Factors Affecting Biosynthesis and Properties. Comprehensive Reviews in Food Science and Food Safety, 2011. 10 (6): p. 303-320.
 
[7]  Wang, J. and G. Mazza, Inhibitory effects of anthocyanins and other phenolic compounds on nitric oxide production in LPS/IFN-gamma-activated RAW 264.7 macrophages. J Agric Food Chem, 2002. 50 (4): p. 850-7.
 
[8]  de Pascual-Teresa, S. and M. Sanchez-Ballesta, Anthocyanins: from plant to health. Phytochemistry Reviews, 2008. 7 (2): p. 281-299.
 
[9]  Wang, L.S. and G.D. Stoner, Anthocyanins and their role in cancer prevention. Cancer Lett, 2008. 269 (2): p. 281-90.
 
[10]  Al-Hajj, N.Q.M., et al., Chemical Composition and Antioxidant Activity of the Essential Oil of Pulicaria Inuloides. Journal of Food and Nutrition Research, 2014. 2 (5): p. 221-227.
 
[11]  Lock, K., et al., The global burden of disease attributable to low consumption of fruit and vegetables: implications for the global strategy on diet. Bull World Health Organ, 2005. 83 (2): p. 100-8.
 
[12]  Crozier, A., et al., Secondary Metabolites in Fruits, Vegetables, Beverages and Other Plant-based Dietary Components, in Plant Secondary Metabolites 2007, Blackwell Publishing Ltd. p. 208-302.
 
[13]  Ahmadiani, N., et al., Anthocyanins contents, profiles, and color characteristics of red cabbage extracts from different cultivars and maturity stages. J Agric Food Chem, 2014. 62 (30): p. 7524-31.
 
[14]  WANG H., C.G., and PRIOR R. L., , Oxygen Radical Absorbing Capacity of Anthocyanins. J Agric Food Chem, 1997. 45: p. 304-309.
 
[15]  Tamura, H. and A. Yamagami, Antioxidative activity of monoacylated anthocyanins isolated from Muscat Bailey A grape. Journal of Agricultural and Food Chemistry, 1994. 42 (8): p. 1612-1615.
 
[16]  Haleem, M.A., et al., Increasing antioxidant intake from fruits and vegetables: practical strategies for the Scottish population. J Hum Nutr Diet, 2008. 21 (6): p. 539-46.
 
[17]  Cho, M.J., et al., Flavonoid glycosides and antioxidant capacity of various blackberry, blueberry and red grape genotypes determined by high-performance liquid chromatography/mass spectrometry. Journal of the Science of Food and Agriculture, 2004. 84 (13): p. 1771-1782.
 
[18]  Zheng, W. and S.Y. Wang, Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries. J Agric Food Chem, 2003. 51 (2): p. 502-9.
 
[19]  Shahidi, F. and P.K. Wanasundara, Phenolic antioxidants. Crit Rev Food Sci Nutr, 1992. 32 (1): p. 67-103.
 
[20]  Mandal, S.M. and S. Dey, LC-MALDI-TOF MS-based rapid identification of phenolic acids. J Biomol Tech, 2008. 19 (2): p. 116-21.
 
[21]  Kajdžanoska, M., V. Gjamovski, and M. Stefova, HPLC-DAD-ESI-MSn identification of phenolic compounds in cultivated strawberries from Macedonia. 2010. Vol. 29. 2010.
 
[22]  Hingse, S., S. Digole, and U. Annapure, Method development for simultaneous detection of ferulic acid and vanillin using high-performance thin layer chromatography. Journal of Analytical Science and Technology, 2014. 5 (1): p. 1-9.
 
[23]  Hong, Y.S., et al., Metabolomics reveals simultaneous influences of plant defence system and fungal growth in Botrytis cinerea-infected Vitis vinifera cv. Chardonnay berries. J Exp Bot, 2012. 63 (16): p. 5773-85.
 
[24]  Benzie, I.F. and J.J. Strain, Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol, 1999. 299: p. 15-27.
 
[25]  Borges, G., et al., Identification of Flavonoid and Phenolic Antioxidants in Black Currants, Blueberries, Raspberries, Red Currants, and Cranberries†. Journal of Agricultural and Food Chemistry, 2009. 58 (7): p. 3901-3909.
 
[26]  Moyer, R.A., et al., Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: vaccinium, rubus, and ribes. J Agric Food Chem, 2002. 50 (3): p. 519-25.
 
[27]  Koca, I. and B. Karadeniz, Antioxidant properties of blackberry and blueberry fruits grown in the Black Sea Region of Turkey. Scientia Horticulturae, 2009. 121 (4): p. 447-450.
 
[28]  Castrejón, A.D.R., et al., Phenolic profile and antioxidant activity of highbush blueberry (Vaccinium corymbosum L.) during fruit maturation and ripening. Food Chemistry, 2008. 109 (3): p. 564-572.
 
[29]  Pimpao, R.C., et al., Analysis of phenolic compounds in Portuguese wild and commercial berries after multienzyme hydrolysis. J Agric Food Chem, 2013. 61 (17): p. 4053-62.
 
[30]  Kader, F., et al., Fractionation and identification of the phenolic compounds of Highbush blueberries (Vaccinium corymbosum, L.). Food Chemistry, 1996. 55 (1): p. 35-40.
 
[31]  Gavrilova, V., et al., Separation, characterization and quantification of phenolic compounds in blueberries and red and black currants by HPLC-DAD-ESI-MSn. J Agric Food Chem, 2011. 59 (8): p. 4009-18.
 
[32]  Zafrilla, P., F. Ferreres, and F.A. Tomas-Barberan, Effect of processing and storage on the antioxidant ellagic acid derivatives and flavonoids of red raspberry (Rubus idaeus) jams. J Agric Food Chem, 2001. 49 (8): p. 3651-5.
 
[33]  Iriwoharn, T.S. and R.E. Wrolstad, Polyphenolic Composition of Marion and Evergreen Blackberries. J Food Sci, 2004. 69 (4): p. FCT233-FCT240.
 
[34]  Henning, W., Phenolics of fruit. XIV. Flavonol glycosides of strawberries (Fragaria × ananassa Duch), raspberries (Rubus idaeus L) and blackberries (Rubus fruticosus L) Z Lebensm Untersuch Forsch, 1981. 173: p. 180-187.