Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2014, 2(10), 671-674
DOI: 10.12691/jfnr-2-10-3
Open AccessArticle

Determination of Aflatoxins in Wheat and Wheat by-products Intended for Human Consumption, Marketed in Rio de Janeiro, Brazil

Felipe Machado Trombete1, , Douglas de Ávila Moraes2, Yuri Duarte Porto1, Thaís Barbosa Santos1, Glória Maria Direito3, Marcelo Elias Fraga3 and Tatiana Saldanha2

1Department of Food Technology, Institute of Technology, Federal Rural University of Rio de Janeiro, Seropédica, Brazil

2Department of Agronomy, Institute of Agronomy, Federal Rural University of Rio de Janeiro, Seropédica, Brazil

3Department of Microbiology and Immunology Veterinary, Institute of Veterinary, Federal Rural University of Rio de Janeiro, Seropédica, Brazil

Pub. Date: September 19, 2014

Cite this paper:
Felipe Machado Trombete, Douglas de Ávila Moraes, Yuri Duarte Porto, Thaís Barbosa Santos, Glória Maria Direito, Marcelo Elias Fraga and Tatiana Saldanha. Determination of Aflatoxins in Wheat and Wheat by-products Intended for Human Consumption, Marketed in Rio de Janeiro, Brazil. Journal of Food and Nutrition Research. 2014; 2(10):671-674. doi: 10.12691/jfnr-2-10-3

Abstract

The consumption of wheat bran, whole-wheat grains and other whole-wheat products has grown in recent years in Brazil. These foods are considered more nutritious than the refined ones and have been associated with a reduced risk of some major chronic diseases. On the other hand, other research, carried out in Brazil, has found different groups of fungi toxins, called mycotoxins, contaminating these wheat products. Among these mycotoxins, are the aflatoxins, a group of genotoxic and carcinogenic compounds produced by Aspergillus spp. This study aimed to determine the levels of aflatoxins B1, B2, G1 and G2 in samples of whole-wheat grains and derivatives, intended for human consumption, marketed in the metropolitan region of Rio de Janeiro, Brazil. One hundred and eight samples of whole-wheat grains (n=35), wheat bran (n=32), whole-wheat flour (n=26) and refined wheat flour (n=15) marketed in hypermarkets, supermarkets and health food stores were analyzed by High Performance Liquid Chromatography with fluorescence detection (HPLC-FL). Thirty-three samples (30.6%) were positive for at least one aflatoxin and the B1 form had the highest prevalence in the samples. The overall average was 0.69 µg/kg and the contamination was the highest in the grain samples, followed by bran, whole-flour and refined flour. Just one sample showed total aflatoxins levels (B1+B2+G1+G2) higher than the limit established by Brazilian legislation (5 µg/kg). The levels found in this study indicated that the presence of aflatoxins in wheat and wheat products consumed in Rio de Janeiro, Brazil, are not a hazard for public health.

Keywords:
mycotoxins AFB1 wheat bran whole-wheat products HPLC

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