Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2014, 2(9), 561-566
DOI: 10.12691/jfnr-2-9-6
Open AccessArticle

Characterization of Different Oil Soapstocks and Their Application in the Lipase Production by Aspergillus niger under Solid State Fermentation

Regiane Ribeiro dos Santos1, Livia Nolasco Macedo Muruci1, Lucielen Oliveira Santos1, Rosemar Antoniassi2, Janine Passos Lima da Silva2 and Mônica Caramez Triches Damaso3,

1Department of Food Technology, Institute of Technology, Rural Federal University of Rio de Janeiro, Caixa Postal, Seropédica, Rio de Janeiro, Brazil

2Embrapa Food Technology, Av. das Américas, Guaratiba, Rio de Janeiro, Brazil

3Embrapa Agroenergy, Parque Estação Biológica, Brasília, Distrito Federal, Brazil

Pub. Date: September 02, 2014

Cite this paper:
Regiane Ribeiro dos Santos, Livia Nolasco Macedo Muruci, Lucielen Oliveira Santos, Rosemar Antoniassi, Janine Passos Lima da Silva and Mônica Caramez Triches Damaso. Characterization of Different Oil Soapstocks and Their Application in the Lipase Production by Aspergillus niger under Solid State Fermentation. Journal of Food and Nutrition Research. 2014; 2(9):561-566. doi: 10.12691/jfnr-2-9-6

Abstract

Lipases are enzymes that catalyze the hydrolysis of the ester linkage in a triacylglycerol oil-water interface, while in non-aqueous environments they catalyze reverse reactions (esterification, transesterification and interesterification). They can be produced by a solid state fermentation (SSF) process adding value to agro-industrial residues as alkaline soapstocks that can be used as a lipid substrate for lipase production. The objective of this study was to characterize the soapstocks of canola, sunflower and corn and use these byproducts for fungal lipase production using the mutant strain 11T53A14 of Aspergillus niger. The work was carried out following 24-1 and 23 experimental designs in the presence and absence of inducers, respectively, aiming to evaluate the influence of some variables in the lipase production. The production by SSF was carried out in aerated columns incubated at 32°C. Among the soapstocks evaluated, the sunflower soapstock was the best inducer for enzyme production, with 201 U/gdm (gram of dry medium) using 108 spores/gm (gram of medium) inoculated on wheat bran humidified with 80 mL of liquid (ammonium sulfate solution) that corresponds to a final concentration of 0.5% nitrogen, and 3% inducer. The best lipase activity obtained without soapstock was 182 U/gdm under the same processing conditions as the experiment using soapstock. Although the results with the highest lipase activity with sunflower soapstock and without inducer are close, the Tukey test showed a significant difference of these results, which proves the importance of lipid substrate to improve the lipase activity.

Keywords:
lipases filamentous fungus solid state fermentation column reactor agroindustrial waste

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