Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Go
Journal of Food and Nutrition Research. 2014, 2(9), 532-538
DOI: 10.12691/jfnr-2-9-1
Open AccessArticle

Spectrophotometric Determination of Trace Elements in various Honey Samples, Collected from different Environments

Afzal Shah1, 2, , Farzana Sikandar1, Imdad Ullah2, 3, Afzal Shah3, Salah Ud-Din Khan4, Usman Ali Rana2, 4 and Thomas McCoy2

1Department of Chemistry, University of Science and Technology, Bannu 28100, Khyber Pakhtunkhwa, Pakistan

2School of Chemistry, Monash University, Clayton, Melbourne, VIC 3800, Australia

3Department of Chemistry, Quaid-i-Azam University, Islamabad 45320, Pakistan

4Deanship of scientific research, College of Engineering, PO Box 800, King Saud University, Riyadh 11421, Saudi Arabia

Pub. Date: August 18, 2014

Cite this paper:
Afzal Shah, Farzana Sikandar, Imdad Ullah, Afzal Shah, Salah Ud-Din Khan, Usman Ali Rana and Thomas McCoy. Spectrophotometric Determination of Trace Elements in various Honey Samples, Collected from different Environments. Journal of Food and Nutrition Research. 2014; 2(9):532-538. doi: 10.12691/jfnr-2-9-1

Abstract

Honey is an important food ingredient due to its nutritive and medicinal values. In this project, varieties of honey samples were collected from different environmental sites in District Bannu. Several spectroscopic techniques were used to determine the composition of a series of trace elements within these samples. Cu, Zn, Al, Fe and Mg composition was determined using flame atomic absorption spectroscopy (FAAS), meanwhile Pb, Ni, Cd and Cr composition was determined by electro-thermal atomic absorption spectroscopy (ETAAS) and graphite atomic absorption spectroscopy (GAAS). Lastly, As, Hg, and Se composition was determined by Hydride generation atomic absorption spectroscopy (HGAAS). The trace elements Al, Zn, Mg, Fe, Cu, Cr, Pb, Cd and Ni were found in considerable abundances. However the amounts of As, Hg and Se were below the detection limits of the instrument ( ≥ 0.1 ppm, ≥ 0.1 ppm and ≥ 0.2 ppm for each element respectively).

Keywords:
trace elements honey food nutrition environment

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  White, J.W., Landis, W.D. “Honey composition and properties”, Beekeeping in the US Agriculture. USA Hand book 1980, 335.
 
[2]  Jusbin, O.S., “Chemical composition and application”, In: Bee Products 1, 25-26, 1996.
 
[3]  Hak, C.G., Kyoo H.M., Gil K.J. “The chemical composition of Korean honey” Korean Food Science and Technology 20, 631-636, 1998.
 
[4]  Ball, David W., “The chemical composition of honey” Chemical education 84, 1643-1646, 2007.
 
[5]  Bansal, V., Medhi, B., Pehndi P.,A remedy rediscovered and its therapeutic utility” Kathmandu University Medical Journal 3, 305-309, 2005.
 
[6]  Gilani, M., Kamal A., Raza, S., Rashid, N., Hameed T., “Comparative study of honey collected from different flora of Pakistan” Biological Sciences 2, 626-627, 2002.
 
[7]  White, J.W., Reithof, M.L., Subers, M.H., Kushnir, I., “Composition of American honey” US Department of agriculture technical bulletin 1261, 11-24, 1962.
 
[8]  Teng, J.H., Wang, T.L., Lin, W.C., Chin, M.T., Chen, Z.S., “Investigation on four heavy metal constituents of commercial restorative Chinese medicines” Food and Drug Analysis 3, 193-202, 1995.
 
[9]  Dundar, M.S., Deryaoglu, N., “Heavy metal determination in outdoor atmosphere dust depositions” Fresenius Environmental Bulletin 14, 185-188, 2005.
 
[10]  Diyrikli, U., Mendil, D., Tuzen, M., Soylak, M, Elci, L., “Trace metal pollution from traffic in Denizli-Turkey during dry season” Biomedical Environmental Sciences 19, 254-261, 2006.
 
[11]  Chen, C.M., Cheng, C.C., Chou, S.S. “Determination of Cu in edible oils by direct graphite furnace atomic absorption spectrometry” Food and Drug Analysis 7, 207-214, 1999.
 
[12]  Chen, Q., Dong, H., “Solvent sublation using dithizone as a ligand for determination of trace elements in water samples” Microchimica Acta 150, 59-65, 2005.
 
[13]  Asraf, W. “Accumulation of heavy metals in kidney and heart tissues of Epinephelus Microdon fish from the Arabian Gulf” Environmental Monitoring Assessment 101, 311-316, 2005.
 
[14]  Fuh, C.B., Lin, H.I, Tsai, H.Y., “Determination of lead, cadmium, chromium and arsenic in 13 herbs of tocolysis formulation using atomic absorption spectrometry” Food ehehehand Drug Analysis 11, 39-45, 2003.
 
[15]  Huang, W.B., “Heavy metal concentrations in the common benthic fishes caught from the coastal waters of eastern Taiwan” Food and Drug Analysis 11, 324-330, 2003.
 
[16]  Narin, I., Tuzen, M., Sari, H., Soylak, M., “Heavy metal content of potato and corn chips from Turkey” Bulletin of Environmental Contamination and Toxicology 74, 1072-1077, 2005.
 
[17]  Demalsy, F., Vincet, B., Beaulieu, F., “Mineral content and geographical origin of Canadian honey” Apidologie 20, 77-91, 1989.
 
[18]  Miret, G., Terrab, M.L., Hernanz, A., Recamales, D.F., Heredia, M.A., “Multivariate correlation between color and mineral composition of honeys and by their botanical origin” Agriculture and Food Chemistry 53, 2574-2580, 2005.
 
[19]  Sevlimli, H., Bayulgen, H., Varinioglu, N., “Determination of trace elements in honey by INAA in Turkey” Radio analytical Nuclear Chemistry Letter 165, 319-325, 1992.
 
[20]  Bogdanov, S., Jurendic, T., Sieber, R., Gallmann, P., “Honey for nutrition and health: A review” American College of Nutrition 27, 677-689, 2008.
 
[21]  Molan, P.C., “Honey for oral health” Dental Research 80 (special issue) 1-13, 2001.
 
[22]  Steinberg, D., Kaine, G., Gedalia, G., “Antibacterial effect of propolis and honey on oral bacteria” American Journal of Dentistry 9, 236-239, 1996.
 
[23]  Armon, P.J., “The use of honey in the treatment of infected wounds” Tropical Doctor 10, 91, 1980.
 
[24]  Cavanagh, D., Ostapowicz, J., Ostapowicz, F. “Radical operation for carcinoma of the vulva” Obstetrics and Gynecology 77, 1037-1040, 1970.
 
[25]  Munstedt, K., Sheybani, B., Hauenschild, B., Bruggmann, A., Bretzel, D., Winter, D., “Effects of basswood honey, honey-comparable glucose-fructose solution, and oral glucose tolerance test solution on serum insulin, glucose, and C-peptide concentrations in healthy subjects” Journal of Medicinal Food 11, 424-428, 2008.
 
[26]  Khali, M.I., Sulaiman, S.A., Boukraa L., “Antioxidant properties of honey and its role in preventing health disorder” The Open Nutraceuticals 3, 6-16, 2010.
 
[27]  Erbilir, F., Erdogrul, O., “Determination of heavy metals in honey in Kahramanmaras city, Turkey” Environmental Monitoring and Assessment 109, 1-3, 2005.
 
[28]  Chen, L.Z., Rui, Y.K., Zhao, J., Ye, Z.H., Xue, X.F., Wang, P., “Application of ICP-MS to detection of trace elements and heavy metals in different kinds of honey” Guang Pu Xue Yu Guang Pu Fen Xi 28, 1403-1405, 2008.
 
[29]  Sanna, G., Maria, I.P., Paola, C.P., “Determination of heavy metals in honey by anodic stripping voltammetry at microelectrodes” Analytical Chimica Acta 415, 165-173, 2000.
 
[30]  Bratu, L., Georgescu, C., “Chemical contamination of bee honey – identifying sensor of the environment pollution” Central European Agriculture 6, 467-470, 2005.
 
[31]  Ioannidou, M.D., Zachariadis, G.A., Anthemidis, A.N., Stratis, J.A., Direct determination of toxic metals in honey and sugar using ICP-AES” Talanta 65, 92-97, 2005.
 
[32]  Fredes, C., Montenegro, G., “Heavy metal and other trace elements contents in Chilean honey” Ciencia e Investigacion Agrarian 33, 50-58, 2006.
 
[33]  Quan, S.X., Jum, T., Guang-guo, X., “Determination of trace elements in water, sea water and biological samples by ICP-AES after pre-concentration with ammonium pyrrolidinedithiocarbamate precipitation” Analytical Chimica Acta 3, 259-263, 1988.
 
[34]  Devillers, J., Dore, J.C., Marenco, M., Ducnene, F.P., “Chemo-metrical analysis of 18 metallic and non-metallic elements found in honeys in France” Agriculture Food Chemistry 50, 5998-6007, 2002.
 
[35]  Tuzen, M., Soylak, M., “Trace heavy metal levels in microwave digested honey samples from middle Anatolia, Turkey” Food and Drug Analysis 13, 343-347.
 
[36]  Conti, M.E., Botre, F., “Honeybees and their products as potential bioindicators of heavy metals contamination” Environmental Monitoring and Assessment 69, 267-282, 2001.
 
[37]  Buldini, P.L., Ricci, L., Sharma, J.L., “Recent applications of sample preparation techniques in food analysis” Journal of Chromatography A 975, 47-70, 2002.
 
[38]  Haswell, S.J., Atomic absorption spectrometry, Theory, Design and Applications, Elsevier, Amsterdam 1991.
 
[39]  Reynolds, R.J., Atomic absorption spectroscopy, Barnes and Noble Inc, New York 1970.
 
[40]  Flaten, T.P., “Aluminium as a risk factor in Alzheimer’s disease, with emphasis on drinking water” Brain Res. Bull. 55, 187-196, 2001.
 
[41]  Chakir, A., Romne, A., Barbagianni, N., Bartoli, D., Ferrazzi, P., “Major and trace elements in different types of Moroccan honeys” Basic and Applied Sciences 5, 223-231, 2011.
 
[42]  Juliana, R.A., Lilyana, Y.D., Maria, L.A., “Palynological, physical and chemical data on honey from the Kazanlak region (Central Bulgaria)” Phytologia Balcanica 15, 107-114, 2009.
 
[43]  Cantarelli, M.A., Pellerano, R.G., Marchevsky, E.J., Camina, J.M., “Quality of honey from Argentina: Study of chemical composition and trace elements” Argentine Chemical Society 96, 33-41, 2008.
 
[44]  Madejczyk, M., Baralkiewicz, D., “Characterization of polish rape and honeydew honey according to their mineral contents using ICP-MS and FAAS / AES” Anal. Chim. Acta 617, 11-17, 2008.
 
[45]  Tuzen, M., “Determination of some metals in honey samples for monitoring environmental pollution” Frensius Environmental Bull 11, 366-370, 2002.
 
[46]  Hernandez, O.H., Fraga, J.M.G., Jimenez, A.I., Jimenez, F., Arias, J.J., “Characterization of honey from the Canary Islands: Determination of the mineral content by atomic absorption spectrophotometry” Food Chemistry 93, 449-458, 2005.
 
[47]  Bogdanov, Haldimann, M., Luginbuhl, W., Gallmann, P., “Minerals in honey: environmental, geographical and botanical aspects” Apicultural Research and Bee World 46, 269-275, 2007.
 
[48]  Ferrer, S.D., Rodriguez, G.O.D., Martinez, J.P.J., Moran, M., “Mineral content of the honey produced in Zulia state” Venezuela, Alan 60, 70-79, 2010.
 
[49]  Matei, N., Birghila, S., Dobrinas, S., Capota, P., “Determination of vitamin C and some trace elements (Ni, Mn, Fe, Cr) in Bee products” Acta. Chim. Slov 51, 169-175, 2004.
 
[50]  Yilmaz, H., Yavuz, O., “Content of some trace metals in honey from South Eastern Anatolia” Food chemistry 65, 475-476, 1999.
 
[51]  Conti, M.E., “Lazio region (Central Italy) honeys: a survey of mineral content and typical quality parameters” Food Control 11, 459-463, 2000.
 
[52]  Pisani, Protano G., Riccobono, F., “Minor and trace elements in different honey types produced in Siena County (Italy)” Food Chemistry 107, 1553-1560, 2008.
 
[53]  Przbylowski, P., Wilczynska, A., “Honey as an environmental marker” Food Chemistry 74, 289-291, 2001.
 
[54]  Belouali, H., Bouakra, M., Hakkou, A.A., “Determination of some major and minor elements in the east of Morocco honeys through inductively coupled plasma optical emission spectrometry” Apicata 43, 17-24, 2008.
 
[55]  Osman, K.A., Al-Doghairi, M.A., Al-Rehiavani, S., Helal, M.I.D., “Mineral contents and physicochemical properties of natural honey produced in Al-Qassim region, Saudi Arabia” Food Agriculture and Environment 5, 142-146, 2007.
 
[56]  Al-Khalifa, A.S., Al-Arify, I.A., “Physicochemical characteristics and pollen spectrum of some Saudi honeys” Food Chemistry 67, 21-25, 1999.
 
[57]  Samimi, A., Maymand, O.E., “Determination of Cadmium and Arsenic pollution by bee honey based on the study on Ja’far abad area from Saveh city from Iran” Water and Geoscience 199-2.