Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2014, 2(8), 457-464
DOI: 10.12691/jfnr-2-8-5
Open AccessArticle

Nutritional Quality of the Walnut Male Inflorescences at Four Flowering Stages

Changlei Wang1, 2, Wene Zhang1, 2, and Xuejun Pan1, 2,

1Guizhou Engineering Research Center for Fruit Crops, Guiyang, China

2Agriculture College, Guizhou University, Guiyang, China

Pub. Date: August 04, 2014

Cite this paper:
Changlei Wang, Wene Zhang and Xuejun Pan. Nutritional Quality of the Walnut Male Inflorescences at Four Flowering Stages. Journal of Food and Nutrition Research. 2014; 2(8):457-464. doi: 10.12691/jfnr-2-8-5


Nutrients, bioactive compounds and antioxidant activities of walnut male inflorescences at four flowering stages were analyzed in this study. The results showed that the male inflorescences were rich in carbohydrate, protein and mineral contents, and had antioxidant activities due to high contents of phenolic and flavonoid compounds. The nutritional compositions were varied at four stages. In the first half stage, including early flowering stage (EFS) and flowering stage (FS), the contents of protein, total amino acids, carbohydrates, antioxidants (phenolic, flavonoid and ascorbic acid) and some minerals (phosphorus, zinc and copper) were higher than that of the second half stage, including pollen-scattering stage (PSS) and later pollen-scattering stage (LPSS). Due to high contents of phenolic and flavonoid at EFS, the highest antioxidant activity was also observed at this stage. However, the higher amounts of crude fat, crude fiber and some mineral elements (potassium, iron, manganese, calcium and magnesium) were found in the second half stage. These results could contribute to using walnut male inflorescences as food, as well as developing new food products, and obtaining the bioactive substances from walnut male flowers.

male inflorescences of walnut proximate composition minerals amino acids bioactive compounds antioxidant activity

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[1]  Mlcek, J. and Rop, O., “Fresh edible flowers of ornamental plants-A new source of nutraceutical foods,” Trends in Food Science & Technology, 22. 561-569. 2011.
[2]  Rop, O., Mlcek, J., Jurikova, T., Neugebauerova, J. and Vabkova, J., “Edible flowers-A new promising source of mineral elements in human nutrition,” Molecules, 17. 6672-6683. 2012.
[3]  Tapia, M.I., Sánchez-Morgado, J.R., García-Parra, J., Ramírez, R., Hemández, T. and González-Gómez, D., “Comparative study of the nutritional and bioactive compounds content of four walnut (Julans regia L.) cultivars,” Journal of Food Composition and Analysis, 31. 232-237. 2013.
[4]  Almeida, I.F., Fernandes, E., Lima, J.L.F.C., Costa, P.C. and Bahia, M.F., “Walnut (Juglans regia) leaf extracts are strong scavengers of pro-oxidant reactive species,” Food Chemistry, 106. 1014-1020. 2008.
[5]  Nabavi, S.F., Ebrahimzadehma, M.A., Nabavi, S.M., Mahmoudi, M. and Keyvani, R.S., “Biological activities of Juglans regia flowers,” Revista Brasileira de Farmacognosia, 21. 465-470. 2011.
[6]  Ebrahimzadeh, M.A., Nabavi, S.F. and Nabavi, S.M., “Antihemolytic activity and mineral contents of Juglans regia L. flowers,” European Review for Medical and Pharmacological Sciences, 17. 1881-1883. 2013.
[7]  Hosseini, S.E., Karimzadeh, K. and Vessal, M., “Effects of a hydroalcoholic extract of walnut male flowers on diabetic rats,” Zahedan Journal of Research in Medical Sciences, 15. 29-32. 2013.
[8]  Zhang, Y.X., Fruit growing for individual tree, China agriculture press, Beijing, 2003, 298.
[9]  AOAC, Official methods of analysis, 18th ed. Association of Official Analytical Chemistry, Washington, DC, 2005.
[10]  National Standard of the People's Republic of China (GB/T 5009), China State Bureau of Quality and Technical Supervision, China, 2003.
[11]  Sánchez-Machado, D.I., Núñez-Gastélum, J.A., Reyes-Moreno, C., Ramírez-Wong, B. and López-Cervantes, J., “Nutritional quality of edible parts of Moringa oleifera,” Food Analytical Methods, 3. 175-180. 2010.
[12]  Park, S., Arasu, M.V., Lee, M.K., Chun, J.H., Seo, J.M., Lee, S.W., Al-Dhabi, N.A. and Kim, S.J., “Quantification of glucosinolates, anthocyanins, free amino acids, and vitamin C in inbred lines of cabbage (Brassica oleracea L.),” Food Chemistry, 145. 77-85. 2014.
[13]  Conde-Hernández, L.A. and Guerrero-Beltrán, J.Á., “Total phenolics and antioxidant activity of Piper auritum and Porophyllum ruderale,” Food Chemistry, 142. 455-460. 2014.
[14]  Feng, S., Luo, Z.S., Zhang, Y.B., Zhong, Z. and Lu, B.Y., “Phytochemical contents and antioxidant capacities of different parts of two sugarcane (Saccharum officinarum L.) cultivars,” Food Chemistry, 151. 452-458. 2014.
[15]  Motamed, S.M. and Naghibi, F., “Antioxidant activity of some edible plants of the Turkmen Sahra region in northern Iran,” Food Chemistry, 119. 1637-1642. 2010.
[16]  Hatamnia, A.A., Abbaspour, N. and Darvishzadeh, R., “Antioxidant activity and phenolic profile of different parts of Bene (Pistacia atlantica subsp. kurdica) fruits,” Food Chemistry, 145. 306-311. 2014.
[17]  Chen, C.Y., Zhao, S.L., Cao, J.X., Zhang, R.Q. and Guo, J.M., “The analyses of nutritional components in flowers of Jugans regia L.,” Food Science, 19. 35-37. 1998.
[18]  Latif, S., Pfannstiel, J., Makkar, H.P.S. and Becker, K., “Amino acid composition, antinutrients and allergens in the peanut protein fraction obtained by an aqueous enzymatic process,” Food Chemistry, 136. 213-217. 2013.
[19]  Arruda, V.A.S., Pereira, A.A.S., Freitas, A.S., Barth, O.M. and Almeida-Muradian, L.B., “Dried bee pollen: B complex vitamins, physicochemical and botanical composition,” Journal of Food Composition and Analysis, 29. 100-105. 2013.
[20]  Ovodova, R.G., Golovchenko, V.V., Popov, S.V., Popova, G.Y., Paderin, N.M., Shashkov, A.S. and Ovodov, Y.S., “Chemical composition and anti-inflammatory activity of pectic polysaccharide isolated from celery stalks,” Food Chemistry, 114. 610-615. 2009.
[21]  Grzeszczuk, M., Wesolowska, A., Jadczak, D. and Jakubowska, B., “Nutritional value of chive edible flowers,” Acta Scientiarum Polonorum-hortorum Cultu, 10. 85-94. 2011.
[22]  Wang, X.M., Zhang, J., Wu, L.H., Zhao, Y.L., Li, T., Li, J.Q., Wang, Y.Z. and Liu, H.G., “Amini-review of chemical composition and nutritional value of edible wild-grown mushroom from China,” Food Chemistry, 151. 279-285. 2014.
[23]  Aboagarib, E.A.A., Yang, R.J., Hua, X. and Siddeeg, A., “Chemical Compositions, Nutritional Properties and Volatile Compounds of Guddaim (Grewia Tenax. Forssk) Fiori Fruits,” Journal of Food and Nutrition Research, 2. 187-192. 2014.
[24]  Singh, G., Kawatra, A. and Sehgal, S., “Nutritional composition of selected green leafy vegetables, herbs and carrots,” Plant Foods for Human Nutrition, 56. 359-364. 2001.
[25]  Velisek, J., Chemie Potravin, 1st ed. OSSIS: Tabor, Czech Republic, 252-324. 2002.
[26]  Shajib, T.I., Kawser, M., Mia, N., Begum, P., Bhattacharjee, L., Hossain, A., Fomsgaard, I.S. and Islam, S.N., “Nutritional composition of minor indigenous fruits: Cheapest nutritional source for the rural people of Bangladesh,” Food Chemistry, 140. 466-470. 2013.
[27]  Campbell, N.A. and Reece, J.B., Biology, 1st ed. Computer Press: Brno, Czech Republic, 2006, 840-911.
[28]  Pan, X.J., Zhang, W.E., Liu, W., Zhang, Z. and Peng, J., (2010) “Fatty acids and amino acids content of walnut kernels in Guizhou,” Southwest China Journal of Agricultural Sciences, 23. 497-501. 2010.
[29]  WHO/FAO, Energy and protein requirements, WHO Technical Report Series, No.522. Geneva, World Health Organization, 1973.
[30]  Isabelle, M., Lee, B.L., Lim, M.T., Koh, W.P., Huang, D.J. and Ong, C.N. “Antioxidant activity and profiles of common fruits in Singapore,” Food Chemistry, 123. 77-84. .
[31]  Isabelle, M., Lee, B.L., Lim, M.T., Koh, W.P., Huang, D.J. and Ong, C.N. “Antioxidant activity and profiles of common vegetables in Singapore,” Food Chemistry, 120. 993-1003. 2010b.
[32]  Sebastian, J., Padayatty, S.J., Katz, A., Wang, Y., Eck, P., Kwon, O., Lee, J.H., Chen, S., Corpe, C., Dutta, A., Dutta, S.K. and Levine, M., “Vitamin C as an antioxidant: Evaluation of its role in disease prevention,” Journal of the American College of Nutrition, 22. 18-35. 2003.
[33]  Zhou, Y., Wang, W., Wang, C.Z. and Pei, D., “Research progress on polyphenols from Juglans plants,” Journal of Nanjing Forestry University (Natural Sciences Edition), 37. 146-152. 2013.
[34]  Kaisoon, O., Siriamornpun, S., Weerapreeyakul, N., Meeso, N., “Phenolic compounds and antioxidant activities of edible flowers from Thailand,” Functional Foods, 3. 88-99. 2011.
[35]  Yang, R.Y., Lin, S. and Kuo, G., “Content and distribution of flavonoids among 91 edible plant species,” Asia Pacific Journal of Clinical Nutrition, 17. 275-279. 2008.
[36]  Halvorsen, B.L., Holte, K., Myhrstad, W., Barikmo, I., Hvattum, E., Remberg, S.F., Wold, A.B., Haffner, K., Baugerød, H., Andersen, L.F., Moskaug, Ø., Jacobs, D.R.Jr. and Blomhoff, R., “A systematic screening of total antioxidants in dietary plants,” Nutrient Requirements, 132. 461-471. 2002.