Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2014, 2(7), 357-362
DOI: 10.12691/jfnr-2-7-5
Open AccessArticle

The Effects of Soybean Trypsin Inhibitor on Free Radicals Levels in Pancreatic Mitochondria of Mice

Chunmei GU1, Shujun Li1, Linlin ZHAO1, Xinxiu SONG1 and Guixin QIN2,

1Institute of Food Science and Engineering, Jilin Agricultural University, Changchun, China

2Institute of Animal Science and Technology, Jilin Agricultural University, Changchun, China

Pub. Date: July 07, 2014

Cite this paper:
Chunmei GU, Shujun Li, Linlin ZHAO, Xinxiu SONG and Guixin QIN. The Effects of Soybean Trypsin Inhibitor on Free Radicals Levels in Pancreatic Mitochondria of Mice. Journal of Food and Nutrition Research. 2014; 2(7):357-362. doi: 10.12691/jfnr-2-7-5

Abstract

This paper aims to explore the effect of soybean trypsin inhibitor(STI) on oxygen free radicals levels in pancreatic mitochondria of mice. 36 male KM mice were randomly divided into three groups and fed with different diets as follows: control group was fed on control diet, STI group was fed on control diet containing STI, and VC (vitamin C) group was fed on STI diet supplemented with vitamin C. Mice were sacrificed by decapitation after being fed for 3 weeks. Pancreas was quickly removed and mitochondria were prepared for determining the oxidative and antioxidative index. The results showed that, compared with mice fed with control diet, malondialdehyde (MDA) content and activity of nitric oxide synthase (NOS) in pancreatic mitochondria of mice fed with STI diet were significantly increased (P < 0.05), the content of glutathione (GSH), total antioxidant capacity (T-AOC), the activities of glutathione peroxidase (GSH-Px), catalase(CAT) and superoxide dismutase (SOD) were significantly dropped. After STI diet was supplemented with antioxidant, the contents of oxidation parameters were significantly decreased (P < 0.05), but their values were still higher than those of control group; the activities of antioxidant parameters in pancreatic mitochondria were significantly increased (P < 0.05), but still lower than those of control group. These results supported that STI could cause oxidative stress in pancreatic mitochondria. In addition, vitamin C could interfere with the role of STI on the organism and effectively improve the status of oxidative stress.

Keywords:
soybean trypsin inhibitor mitochondria free radical mice pancreas

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