Bile Salts Deconjugation Using Microencapsulated Lactic Acid Bacteria Isolated from Handmade Yogurt

Gulcin Alp Avcı^1,

¹Department Molecular Biology and Genetics/ Molecular Microbiology, Hitit University, Corum, Turkey

Cite this paper:
Gulcin Alp Avcı. Bile Salts Deconjugation Using Microencapsulated Lactic Acid Bacteria Isolated from Handmade Yogurt. Journal of Food and Nutrition Research. 2014; 2(7):340-343. doi: 10.12691/jfnr-2-7-2

Abstract

The preferential aim of this study is to evaluate the deconjugation of sodium taurocholate by L. delbrueckii subsp. bulgaricus and S. thermophilus. As a supportive objective, determination of advantage of microencapsulation effects on deconjugation of sodium taurocholate of these strains, deconjugation property of which is the highest. Deconjugation of sodium taurocholate of L. delbrueckii subsp. bulgaricus (HL6, HL9 and HL14) and S. thermophilus (HS7, HS12 and HS24) strains was determined and strains with the highest deconjugation property were used for microencapsulation study. Then, viability and deconjugation of sodium taurocholate of microencapsulated strains were determined. The amount of deconjugated sodium taurocholate for Lactobacillus and Streptococcus species was found as 1.0 -1.4 mg/mL and 0.9-1.3 mg/mL, respectively. The amount of deconjugated encapsulated HL6 and HS12 strains was found 1.5 and 1.4 mg/mL, respectively. Either the viability values of encapsulated strains or the ability of deconjugation of sodium taurocholate by these strains was found much higher than those of free strains. For dairy products which were planned to be used in probiotic industry, microencapsulated strains can be recommended because of their higher viability and deconjugation ability.

Keywords:
L. delbrueckii subsp. bulgaricus S. thermophilus bile salt hydrolase deconjugation microencapsulation

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Soomro A.H, Masud J, Anwaar K, “Role of Lactic Acid Bacteria (LAB) in Food Preservation and Human Health”, Pakistan Nutrition, 1, 20-24, 2002.
[2]	Pereira D.I.A, Gibson G.R, “Effects of Consumption of Probiotics and Prebiotics on Serum Lipid Levels in Humans”, Crit Rev Biochem Mol Biol 37(4), 259-281, 2002.
[3]	Nguyen T.D.T, Kang J.H, Lee M.S, “Characterization of Lactobacillus plantarum PH04, a potential probiotic bacterium with cholesterol-lowering effects”, Int J Food Microbiol, 113, 358-361, 2007.
[4]	Noh D.O, Kim S.H, Gilliland S.E, “Incorporation of cholesterol into the cellular membrane of Lactobacillus acidophilus ATCC 43121”. J Dairy Sci, 80, 3107-3113, 1997.
[5]	Kumar R, Grover S, Batish V.K, “Hypocholesterolaemic effect of dietary inclusion of two putative probiotic bile salt hydrolaseproducing Lactobacillus plantarum strains in prague-Dawley rats”, Br J Nutr, 105, 1-12, 2010.
[6]	Kim G.B, Yi S.H, Lee B.H, “Purification and characterization of three different types of bile salt hydrolase from Bifidobacterium strains”, J Dairy Sci, 87, 258-266, 2004.
[7]	Noriega L, Cuevas I, Margolles A, de los Reyes-Gavila´n C.G, “Deconjugation and bile salts hydrolase activity by Bifidobacterium strains with acquired resistance to bile”. Int Dairy, 16: 850-855, 2006.
[8]	Aslim B, Alp G, “The effect of immobilization on some probiotic properties of Streptococcus thermophilus strains”, Ann Microbiol, 59 (1): 127-132, 2009.
[9]	Borgogna M, Bellich B, Zorzin L, Lapasin R, Cesaro A, “Food microencapsulation of bioactive compounds: rheological and thermal characterisation of non-conventional gelling system”, Food Chemistry, 122(2), 416-423, 2010.
[10]	Doleyres Y, Lacroix C, “Technologies with free and immobilized cells for probiotic bifidobacteria production and protection”, Int Dairy J, 15, 973-988, 2005.
[11]	Grosso C.R.F, Trindade C.S.F, “Stability of free and immobilized Lactobacillus acidophilus and Bifidobacterium lactis in acidified milk and of immobilized B. lactis in yoghurt”, Braz J Microbiol, 35, 151-156, 2004.
[12]	Walker D.K, Gilliland S.E, “Relationships among bile tolerance, bile salt deconjugation, and assimilation of cholesterol by Lactobacillus acidophilus”, J Dairy Sci, 76, 956-961, 1993.
[13]	Irvin J.L, Johnson C.G, Kopala J, “A photometric method of the determination of cholates in bile and blood”, J Biol Chem, 153, 439-457, 1944.
[14]	Aslim B, Beyatli Y, Yuksekdag Z.N, “Productions and monomer compositions of exopolysaccharides by Lactobacillus delbrukii subsp. bulgaricus and Streptococcus thermophilus strains isolated from traditional home-made yoghurts and raw milk”, Int J Food Sci Technol, 41, 973-979, 2006.
[15]	Gopal S.R, Hylemon P.B, “Purification and characterization of bile salt hydrolase from Clostridium perfringens”, Journal of Lipid Research, 29, 1079-1085, 1988.
[16]	Sridevi N, Vishwe P, Prabhune A, “Hypocholesteremic effect of bile salt hydrolase from Lactobacillus buchneri ATCC 4005”, Food Res Int, 42, 516-520, 2009.
[17]	Yildiz G.G, Ozturk M, Aslim B “Identification of Lactobacillus strains from breast-fed infant and investigation of their cholesterol-reducing effects”, World J Microbiol Biotechnol, 27(10), 2397-2306, 2011.
[18]	Corzo G, Gilliland S.E, “Bile salt hydrolase activity of three strains of Lactobacillus acidophilus”, J Dairy Sci, 82, 472-480, 1999.
[19]	Kailasapathy K, “Microencapsulation of probiotic bacteria: technology and potential applications”, Curr. Issues Intest. Microbiol, 3, 39-48, 2002.
[20]	Gbassi G.K, Vandamme T, “Probiotic encapsulation technology: from microencapsulation to release into the gut”, Pharmaceutics, 4, 149-163, 2012.
[21]	Jones M.L, Chen H, Ouyang W, Metz T, Prakash S, “Microencapsulated genetically engineered Lactobacillus plantarum 80 (pCBH1) for bile acid deconjugation and its implication in lowering cholesterol” J Biomed Biotechnol, 1, 61-69, 2004.
[22]	Tanaka H, Doesburg K, Iwasaki T, Mierau I, “Screening of lactic acid bacteria for bile salt hydrolase activity”, J Dairy Sci, 82, 2530-2535, 1999.
[23]	De Smet I, van Hoorde L, Woestyne M.V, Christiaens H, Verstraete W, “Significance of bile salt hydrolytic activities of lactobacilli”, J Appl Bacteriol, 79, 292-301, 1995.
[24]	Grill J.P, Perrin S, Schneider F, “Bile salt toxicity to some bifidobacteria strains: role of conjugated bile salt hydrolase and pH”, Can J Biochem, 46, 878-884, 2000.