Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2014, 2(6), 277-280
DOI: 10.12691/jfnr-2-6-2
Open AccessArticle

Protective Effects of Berry Extracts on Hydrogen Peroxide-induced Rat Brain Neuronal Cell Damage In Vitro

Chang-Ho Jeong1, Chi-Woen Jang1, Dong-Chan Kum1, Koo Yul Lee1, Seung Yuan Lee2, Sun Jin Hur2 and Seung-Jae Lee2, 3,

1Wooyang Frozen Food Co., LTD., Chungnam, Korea

2Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea

3Functional Food Design Research Center, Faculty of Agriculture, Kyushu University, Fukuoka, Japan

Pub. Date: June 09, 2014

Cite this paper:
Chang-Ho Jeong, Chi-Woen Jang, Dong-Chan Kum, Koo Yul Lee, Seung Yuan Lee, Sun Jin Hur and Seung-Jae Lee. Protective Effects of Berry Extracts on Hydrogen Peroxide-induced Rat Brain Neuronal Cell Damage In Vitro . Journal of Food and Nutrition Research. 2014; 2(6):277-280. doi: 10.12691/jfnr-2-6-2


The purpose of this study was to evaluate the antioxidative activities and protective effects of boysenberry, black currant, and blueberry extracts on neuronal cells. We found that berry extracts had different levels of antioxidant efficacy in various assays, including cell viability assays using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT). In addition, reactive oxygen species (ROS) were measured using 2',7'–dichlorofluorescein diacetate (DCF-DA) and flow cytometry analysis. Fruit extracts possessed strong antioxidant activities as measured using the malondialdehyde (MDA) assays. These extracts effectively inhibited lipid peroxidation. Black currant fruit extract significantly reduced intracellular ROS accumulation resulting from hydrogen peroxide treatment in PC12 cells. Black currant fruit more effectively inhibited lactate dehydrogenase (LDH) release into the medium than other tested fruit extracts did. The present results indicate that fruit extracts have the potential to protect neurons from hydrogen peroxide-induced cell damage and should be considered as a prospective functional food and therapeutic product.

antioxidant hydrogen peroxide neuro-protection boysenberry black currant blueberry

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