Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2023, 11(3), 244-250
DOI: 10.12691/jfnr-11-3-9
Open AccessArticle

Hazard Analysis Critical Control Point (HACCP) System Implementation in the Dairy Supply Chain (Karachi-Pakistan)

Sayed Zaheer Abbas1, 2, Muhammad Naseem Khan1, , Anjum Zehra Naqvi2, Kashan Kaif3, Nargis Tabassum4, Zulfiqar Ali Mirani1, Abdul Basit Khan1, Anila Siddiqui1 and Mazahir Hussain5

1Microbiology Section, Food and Marine Resources Research Centre, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex Karachi - 75280, Pakistan

2Department of Microbiology, University of Karachi – 75270, Sindh, Pakistan

3Department of Chemistry, Faculty of Science and Technology, NOVA University of Lisbon, 2829-516, Caparica, Portugal

4Department of Pharmaceutical Chemistry, Federal Urdu University of Art, Science and Technology, Karachi – 75300, Pakistan

5Department of Bioscience, Shaheed Zulfikar Ali Bhutto Institute of Science and Technology, Karachi - 75600, Pakistan

Pub. Date: March 19, 2023

Cite this paper:
Sayed Zaheer Abbas, Muhammad Naseem Khan, Anjum Zehra Naqvi, Kashan Kaif, Nargis Tabassum, Zulfiqar Ali Mirani, Abdul Basit Khan, Anila Siddiqui and Mazahir Hussain. Hazard Analysis Critical Control Point (HACCP) System Implementation in the Dairy Supply Chain (Karachi-Pakistan). Journal of Food and Nutrition Research. 2023; 11(3):244-250. doi: 10.12691/jfnr-11-3-9


The study's objective was to implement the HACCP plan in a local dairy supply chain in Karachi, Pakistan, to eliminate or reduce the risks for safe and high-quality milk available to the general public and to assess the degree of conformance to food safety and to look into the actual complexity involved in the HACCP implementation process. Different food safety hazards may enter the dairy supply chain during milk production, processing, shipping, storage, and serving, which may have an impact on milk safety and quality. The total average bacterial count 8.5x108±0.18 CFU/mL, Coliforms 83.1±25 CFU/mL, E. coli 96.1±4.1 CFU/mL, and Staph. aureus 27±9.7 CFU/mL were recorded in the milk samples before the HACCP implementation. Although after the HACCP implementation, the count reduced to a considerable level as; 53% (2.9x104±0.15CFU/mL) reduction was noted in the total bacterial count, 80.4% (16±4.8 CFU/mL) in Coliform count, 77% (18.4±0.90 CFU/mL) in E. coli count and 83% (4.2±1.5 CFU/mL) in Staph. aureus count in the final product. Whereas, Salmonella and Listeria were not detected in any milk sample before and after HACCP system implementation. The results reflected the remarkable improvement in milk microbial hygiene. This means that our HACCP system was effective, however, continual improvement may be achieved by periodic monitoring of risks.

CCPs dairy food safety HACCP supply chain

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[1]  Sattar, A. Milk Production in Pakistan. Pakistan Institute of Development Economics (PIDE) 2020; Available from:
[2]  Economic Adviser's Wing. The Pakistan Economic Survey 2019-20, 2020. Government of Pakistan.
[3]  Jan, T., Yadav, K. and Borude, S. Study of HACCP implementation in milk processing plant at Khyber Agro Pvt. Ltd in Jammu & Kashmir. J Food Process Technol, 7(610). 2. 2016.
[4]  Owusu-Kwarteng, J., Akabanda, F., Agyei, D. and Jespersen, L. Microbial safety of milk production and fermented dairy products in Africa. Microorganisms, 8(5).752. 2020.
[5]  Javed, A. Food borne health issues and their relevance to Pakistani Society. American Academic Scientific Research Journal for Engineering, Technology, and Sciences, 26(4). 235-251. 2016.
[6]  Cusato, S., Gameiro, A. H., Corassin, C. H., Santana, A. S., Cruz, G. A., Faria, J. A. and Oliveira, C. A. Food safety systems in a small dairy factory: Implementation, major challenges, and assessment of systems' performances. Foodborne pathogens and disease, 10(1). 12. 2013.
[7]  Shkromada, O., Skliar, O., Paliy, A., Ulko, L., Gerun, I., Naumenko, O., Ishchenko, K., Kysternaet, O., Musiienko, O. and Paliy, A. Development of measures to improve milk quality and safety during production. Eastern-European Journal of Enterprise Technologies, 3 (11). 30-39. 2019.
[8]  Marri, N., Losito, F., Boffe, L., Giangolini, G., Amatiste, S., Murgia, L., Arienzo, A. and Antonini, G. Rapid microbiological assessment in raw milk: validation of a rapid alternative method for the assessment of microbiological quality in raw milk. Foods, 9(9). 1186. 2020.
[9]  Muhammad, K., Altaf, A., Hanif, A., Anjum, A. A. and Tipu, M. Y. Monitoring of hygienic status of raw milk marketed in Lahore City, Pakistan. The Journal of Animal & Plant Sciences, 19(2). 74-77. 2009.
[10]  Melini, F., Melini, V., Luziatelli, F. and Ruzzi, M. Raw and heat-treated milk: From public health risks to nutritional quality. Beverages, 3(4). 54. 2017.
[11]  Quintana, Á.R., Sesena, S., Garzon, A. and Arias, R. Factors affecting levels of airborne bacteria in dairy farms: A review. Animals, 10(3). 526. 2020.