Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2021, 9(6), 304-312
DOI: 10.12691/jfnr-9-6-6
Open AccessArticle

Effect of CNU091, a Lactic Acid Bacterium, Isolated from Fermented Mustard on Antimicrobial Activity, in vitro and in vivo

Chen-Kai Chang1, Shih-Ying Chen2, Shu-Chen Wang1, , Chih Kwang Chiu1 and Pin-Der Duh1,

1Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, Republic of China

2Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, Republic of China

Pub. Date: June 29, 2021

Cite this paper:
Chen-Kai Chang, Shih-Ying Chen, Shu-Chen Wang, Chih Kwang Chiu and Pin-Der Duh. Effect of CNU091, a Lactic Acid Bacterium, Isolated from Fermented Mustard on Antimicrobial Activity, in vitro and in vivo. Journal of Food and Nutrition Research. 2021; 9(6):304-312. doi: 10.12691/jfnr-9-6-6


The antimicrobial activity of lactic acid bacteria (LAB) isolated from fermented mustard were investigated in vitro and in vivo. One hundred and fifty nine strains of LAB isolated from traditional Taiwan fermented mustard were evaluated for their adherence ability, acid and bile tolerance and antimicrobial activity in vitro. In addition, Wistar rats were orally administered with soy fermented products fermented with CNU091 for their antimicrobial activity in vivo. Fecal and fecal of cecum samples were collected to determine number of beneficial bacteria Bifidobacterium spp. and harmful bacteria Clostridium perfringens by classical plate count and by PCR amplification. Of the strains, CNU091 was acid- and bile-tolerant, and significantly higher adherence to Caco-2 cells compared with the commercial probiotic. CNU091 identified as Lactobacillus plantarum with 16S rRNA sequences could inhibit the growth of pathogenic bacteria, including Escherichia coli, Salmonella Enteritidis, and Staphylococcus aureus in vitro. Fecal and fecal of cecum samples were collected to determine number of beneficial bacteria Bifidobacterium spp. and harmful bacteria Clostridium perfringens, CNU091 had a significant antimicrobial activity compared to the control. The villus height (Vh) / crypt depth (Cd) ratio were increased, which suggested CNU091 modulated intestinal mucin composition. The results implicated CNU091 on antibacterial activity may be regarded as a biological activity and have potential for improving the intestinal health.

antimicrobial potential fermented mustard lactic acid bacteria probiotic effect

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