Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2021, 9(4), 199-205
DOI: 10.12691/jfnr-9-4-5
Open AccessArticle

Stability of the Trolox Equivalent Antioxidant Capacity (TEAC) of Human Milk Frozen at –20°C for 6 Months

Krongporn Ongprasert1, , Penprapa Siviroj1, Jetsada Ruangsuriya2, Rungnapa Malasao1, Chotiros Phanpong3 and Darunnee Limtrakul3

1Department of Community Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, 50200

2Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, 50200

3Regional Health Promotion Center 1, Chiang Mai, Thailand, 50100

Pub. Date: April 19, 2021

Cite this paper:
Krongporn Ongprasert, Penprapa Siviroj, Jetsada Ruangsuriya, Rungnapa Malasao, Chotiros Phanpong and Darunnee Limtrakul. Stability of the Trolox Equivalent Antioxidant Capacity (TEAC) of Human Milk Frozen at –20°C for 6 Months. Journal of Food and Nutrition Research. 2021; 9(4):199-205. doi: 10.12691/jfnr-9-4-5


The practice of storing human breast milk (HM) for later use has substantially increased over the last few decades because it allows lactating mothers to continue breastfeeding despite lifestyle changes. Several studies have focused on the safety and nutritional value of milk during the period from the time of storage to that of consumption. To date, few studies have investigated the effect of the storage duration on the antioxidant capacities of stored HM. This study aimed to evaluate the impact of different storage times on the Trolox equivalent antioxidant capacity (TEAC) of frozen HM (−20°C). HM samples from 186 healthy women were collected from women who had term deliveries and a lactating duration between 1 and 24 months. The TEAC values of the frozen HM stored for 1, 2, 4, and 6 months were determined using a working 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) solution. The mean TEAC level of an HM sample, which was frozen and stored at −20 °C for 1 month (1.86 ± 0.82 mM), was compared to those of samples stored for different lengths of time. No significant difference in the mean TEAC values was observed after frozen storage for 2 months (1.88 ± 0.86 mM), 4 months (1.96 ± 0.93 mM), and 6 months (1.91 ± 1.01 mM) (p > 0.05). These results suggest that in cases in which HM needs to be stored for more than 1 month, the storage of frozen HM at −20 °C appears to be the optimal condition for preserving the antioxidant capacity of HM for up to 6 months.

antioxidant breast milk frozen milk human milk Trolox equivalent antioxidant capacity

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