Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Journal of Food and Nutrition Research. 2019, 7(12), 845-850
DOI: 10.12691/jfnr-7-12-5
Open AccessArticle

The Anti-Aging Activities against Oxidative Damages of Rosa roxburghii and Multi-Fruit Concentrate Drink

Cheng Liu1, Leong-Perng Chan2, 3, and Chia-Hua Liang4,

1Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan

2Department of Otorhinolaryngology-Head and Neck Surgery, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan

3Department of Otorhinolaryngology-Head and Neck Surgery, Kaohsiung Medical University Hospital, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

4Department of Cosmetic Science and Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan

Pub. Date: December 23, 2019

Cite this paper:
Cheng Liu, Leong-Perng Chan and Chia-Hua Liang. The Anti-Aging Activities against Oxidative Damages of Rosa roxburghii and Multi-Fruit Concentrate Drink. Journal of Food and Nutrition Research. 2019; 7(12):845-850. doi: 10.12691/jfnr-7-12-5


This research unveils potential of the Rosa roxburghii formula drink for anti-oxidation, anti-aging, anti-inflammation, and the reduction of cancer risk. We recruited 40 high risk subjects (i.e., chronic smoker or drinker) and required them to have daily supplement of R. roxburghii formula drink over 12 weeks to investigate the long-term effect on health improvement. We discovered that the drink could increase mitochondrial activity (elevated nicotinamide adenine dinucleotide (NADH) level), alleviate inflammatory response by the reduction of tumor necrosis factor alpha (TNF-α) level, and diminish the expression of cancer biomarkers of 8-hydroxy-2’-deoxyguanosine (8-OHdG) and carcinoembryonic antigen (CEA). As a result, this is the first study to elucidate the synergistic effect of R.roxburghii and other five kinds of fruits on alleviation oxidative damages.

Rosa roxburghii anti-aging anti-oxidation anti-inflammation drink

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


Figure of 4


[1]  Varani, J., Dame, M.K., Rittie, L., Fligiel, S.E., Kang, S., Fisher, G.J., Voorhees, J.J., “Decreased collagen production in chronologically aged skin: roles of age-dependent alteration in fibroblast function and defective mechanical stimulation”, Am J Pathol, 168(6). 1861-1868. Jun 2006.
[2]  Montecino-Rodriguez, E., Berent-Maoz, B., Dorshkind, K., “Causes, consequences, and reversal of immune system aging”, J Clin Invest, 123(3). 958-965. Mar 2013.
[3]  Bratic, I., Trifunovic, A., “Mitochondrial energy metabolism and ageing”, Biochim Biophys Acta, 1797(6-7). 961-967. Jun-Jul 2010.
[4]  Liguori, I., Russo, G., Curcio, F., Bulli, G., Aran, L., Della-Morte, D., Gargiulo, G., Testa, G., Cacciatore, F., Bonaduce, D., Abete, P., "Oxidative stress, aging, and diseases", Clin Interv Aging. 26; 13. 757-772. Apr 2018.
[5]  Gil Del Valle, L., "WITHDRAWN: Oxidative stress in aging: Theoretical outcomes and clinical evidences in humans", Biomed Pharmacother. 1. 1-7. Sep 2010.
[6]  Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., Bitto, A., "Oxidative stress: Harms and benefits for human health", Oxid Med Cell Longev. 2017. 8416763. Jul 2017.
[7]  Starkov, A.A., "The role of mitochondria in reactive oxygen species metabolism and signaling", Ann N Y Acad Sci. 1147. 37-52. Dec 2008.
[8]  Hekimi, S., Wang, Y., Noë, A., "Mitochondrial ROS and the effectors of the intrinsic apoptotic pathway in aging cells: The discerning killers", Front Genet. 14;7. 161. Sep 2016.
[9]  Valavanidis, A., Vlachogianni, T., Fiotakis, K., “Tobacco smoke: involvement of reactive oxygen species and stable free radicals in mechanisms of oxidative damage, carcinogenesis and synergistic effects with other respirable particles”, Int J Environ Res Public Health. 6(2). 445-462. Feb 2009.
[10]  Lodovici, M., Bigagli, E., “Oxidative stress and air pollution exposure”, J Toxicol. 2011. 487074. Aug 2011.
[11]  Zafar, M.S., Quarta, A., Marradi, M., Ragusa, A., “Recent developments in the reduction of oxidative stress through antioxidant polymeric formulations”, Pharmaceutics. 1; 11(10). Oct 2019.
[12]  Dröge, W., “Free radicals in the physiological control of cell function”, Physiol Rev. 82(1). 47-95. Jan 2002.
[13]  Ogawa, K., Suzuki, K., Okutsu, M., Yamazaki, K., Shinkai, S., : “The association of elevated reactive oxygen species levels from neutrophils with low-grade inflammation in the elderly”, Immun Ageing. 24; 5. 13. Oct 2008.
[14]  Canli, Ö., Nicola,s A.M., Gupta, J., Finkelmeier, F., Goncharova, O., Pesic, M., Neumann, T., Horst, D., Löwer, M., Sahin, U., Greten, F.R., “Myeloid cell-derived reactive oxygen species induce epithelial mutagenesis”, Cancer Cell. 11; 32(6). 869-883. e5. Dec 2017.
[15]  Chung, H.Y., Kim, D.H., Lee, E.K., Chung, K.W., Chung, S., Lee, B., Seo, A.Y., Chung, J.H., Jung, Y.S., Im, E., Lee, J., Kim, N.D., Choi, Y.J., Im, D.S., Yu, B.P., “Redefining chronic inflammation in aging and age-related diseases: Proposal of the senoinflammation concept”, Aging Dis. 1;10(2). 367-382. Apr 2019.
[16]  Zhang, J., Wang, X., Vikash, V., Ye, Q., Wu, D., Liu, Y., Dong, W., “ROS and ROS-mediated cellular signaling”, Oxid Med Cell Longev. 2016. 4350965. Feb 2016.
[17]  Chandrasekaran, A., Idelchik, M.D.P.S., Melendez, J.A., “Redox control of senescence and age-related disease”, Redox Biol. 11. 91-102. Apr 2017.
[18]  Siti, H.N., Kamisa,h Y., Kamsiah, J., “The role of oxidative stress, antioxidants and vascular inflammation in cardiovascular disease (a review)”, Vascul Pharmacol.71. 40-56. Aug 2015.
[19]  Chen, L., Liu, B., “Relationships between stress granules, oxidative stress, and neurodegenerative diseases”, Oxid Med Cell Longev. 2017. 1809592. Jan 2017.
[20]  Luceri, C., Bigagli, E., Femia, A.P., Caderni, G., Giovannelli, L., Lodovici, M., “Aging related changes in circulating reactive oxygen species (ROS) and protein carbonyls are indicative of liver oxidative injury”, Toxicol Rep. 21;5. 141-145. Dec 2017.
[21]  Wilson, D.W., Nash, P., Buttar, H.S., Griffiths, K., Singh, R., De Meester, F., Horiuchi, R., Takahashi, T., "The role of food antioxidants, benefits of functional foods, and influence of feeding habits on the health of the older person: An overview", Antioxidants. 6(4). 81. Oct 2017.
[22]  Liu, M.H., Zhang, Q., Zhang, Y.H., Lu, X.Y., Fu, W.M., He, J.Y., "Chemical analysis of dietary constituents in Rosa roxburghii and Rosa sterilis fruits", Molecules. 9;21(9). Sep 2016.
[23]  Wang, L., Zhang, B., Xiao, J., Huang, Q., Li, C., Fu, X., “Physicochemical, functional, and biological properties of water-soluble polysaccharides from Rosa roxburghii Tratt fruit”, Food Chem. 30;249. 127-135. May 2018.
[24]  Chen, G., Kan, J., “Characterization of a novel polysaccharide isolated from Rosa roxburghii Tratt fruit and assessment of its antioxidant in vitro and in vivo”, Int J Biol Macromol. 107(Pt A). 166-174. Feb 2018.
[25]  Liu, W., Li, S.Y., Huang, X.E., Cui, J.J., Zhao, T., Zhang, H., “Inhibition of tumor growth in vitro by a combination of extracts from Rosa roxburghii Tratt and Fagopyrum cymosum, Asian Pac J Cancer Prev. 13(5). 2409-2414. May 2012.
[26]  Xiang, Q.S., Xiao, H.F., Gao, S.B., Wen, M.T., Jia, G.L., Ren, X.M., Liu, X.B., “In vitro antioxidant properties of Rosa roxburghii aqueous extracts”, AJCS. 6(5). 854-860. 2012.
[27]  Xu, S.J., Zhang, F., Wang, L.J., Hao, M.H., Yang, X.J., Li, N.N., Ji, H.L., Xu, P., “Flavonoids of Rosa roxburghii Tratt offers protection against radiation induced apoptosis and inflammation in mouse thymus”, Apoptosis. 23(9-10). 470-483. Oct 2018.
[28]  Xu, P., Liu, X., Xiong, X., Zhang, W., Cai, X., Qiu, P., Hao, M., Wang, L., Lu, D., Zhang, X., Yang, W., “Flavonoids of Rosa roxburghii Tratt exhibit anti-apoptosis properties by regulating PARP-1/AIF”, J Cell Biochem. 118(11). 3943-3952. Nov 2017.
[29]  Chen, Y., Liu, Z.J., Liu, J., Liu, L.K., Zhang, E.S., Li, W.L., “Inhibition of metastasis and invasion of ovarian cancer cells by crude polysaccharides from rosa roxburghii tratt in vitro”, Asian Pac J Cancer Prev. 15(23). 10351-10354. Dec 2014.
[30]  Lin, H.Y., Su, Y.H., “Method for cell rejuvenation of using Rosa roxburghii fruit extracts”, US 2019/0160131 A1. 2019.
[31]  West, B.J., Deng, S., Isami, F., Uwaya, A., Jensen, C.J., "The potential health benefits of noni juice: a review of human intervention studies", Foods. 7(4). 58. Apr 2018.
[32]  Wiesing, U., "Should performance-enhancing drugs in sport be legalized under medical supervision", Sports Med. 1; 41(2). 167-76. Feb 2011.
[33]  Yang, J., Gadi, R., Thomson, T., “Antioxidant capacity, total phenols, and ascorbic acid content of noni (Morinda citrifolia) fruits and leaves at various stages of maturity”, Micronesica. 41(2). 167-176. 2011.
[34]  Wang, M.Y., Lutfiyya, M.N., Weidenbacher-Hoper, V., Anderson, G., Su, C.X., West, B.J., “Antioxidant activity of noni juice in heavy smokers”, Chem Cent J. 6;3. 13. Oct 2009.
[35]  Wang, M.Y., Peng, L., Lutfiyya, M.N., Henley, E., Weidenbacher-Hope,r V., Anderson, G., "Morinda citrifolia (noni) reduces cancer risk in current smokers by decreasing aromatic DNA adducts", Nutr Cancer. 61(5). 634-639. 2009.
[36]  Zielińska, A., Nowak, I., “Abundance of active ingredients in sea-buckthorn oil”, Lipids Health Dis. 19; 16(1). 95. May 2017.
[37]  Fatima, T., Snyder, C.L., Schroeder, W.R., Cram, D., Datla, R., Wishart, D., Weselake, R.J., Krishna, P., “Fatty acid composition of developing sea buckthorn (Hippophae rhamnoides L.) berry and the transcriptome of the mature seed”, PLoS One. 7(4). e34099. Apr 2012.
[38]  Pang, X., Zhao, J., Zhang, W., Zhuang, X., Wang, J., Xu, R., Xu, Z., Qu, W., "Antihypertensive effect of total flavones extracted from seed residues of Hippophae rhamnoides L. in sucrose-fed rats", J Ethnopharmacol. 8; 117(2). 325-331. May 2008.
[39]  Zhang, L., Huang, D., Kondo, M., Fan, E., Ji, H., Kou, Y., Ou, B., "Novel high-throughput assay for antioxidant capacity against superoxide anion", J Agric Food Chem. 8; 57(7). 2661-2667. Apr 2009.
[40]  Pelzmann, B., Hallström, S., Schaffer, P., Lang, P., Nadlinger, K., Birkmayer, G.D., Vrecko, K., Reibnegger, G., Koidl, B., "NADH supplementation decreases pinacidil-primed I K ATP in ventricular cardiomyocytes by increasing intracellular ATP", Br J Pharmacol. 139(4). 749-754. Jun 2003.
[41]  Zhao, Y., Jin, J., Hu, Q., Zhou, H.M., Yi, J., Yu, Z., Xu, L., Wang, X., Yang, Y., Loscalzo, J., "Genetically encoded fluorescent sensors for intracellular NADH detection", Cell Metab. 5;14(4). 555-66. Oct 2011.
[42]  de Picciotto, N.E., Gano, L.B., Johnson, L.C., Martens, C.R., Sindler, A.L., Mills, K.F., Imai, S., Seals, D.R., "Nicotinamide mononucleotide supplementation reverses vascular dysfunction and oxidative stress with aging in mice", Aging Cell.15(3). 522-530. Jun 2016.
[43]  Dong, Y., Digman, M.A., Brewer, G.J., "Age- and AD-related redox state of NADH in subcellular compartments by fluorescence lifetime imaging microscopy", Geroscience. 41(1). 51-67. Feb 2019.
[44]  Kirwan, J.P., Krishnan, R.K., Weaver, J.A., Del Aguila, L.F., Evan,s W.J., "Human aging is associated with altered TNF-alpha production during hyperglycemia and hyperinsulinemia", Am J Physiol Endocrinol Metab. 281(6). E1137-43. Dec 2001.
[45]  Beckman, K.B., Ames, B.N., "The free radical theory of aging matures", Physiol Rev. 78(2). 547-581. Apr 1998.
[46]  Guo, C., Li, X., Ye, M., Xu, F., Yu, J., Xie, C., Cao, X., Guo, M., Yuan, Y., Zheng, S., “Discriminating patients with early-stage breast cancer from benign lesions by detection of oxidative DNA damage biomarker in urine”, Oncotarget. 12; 8(32). 53100-53109. May 2017.
[47]  Kirkham, P.A., Barnes, P.J., “Oxidative stress in COPD”, Chest. 144(1). 266-273. Jul 2013.
[48]  Tucker, P.S., Dalbo, V.J., Han, T., Kingsley, M.I., “Clinical and research markers of oxidative stress in chronic kidney disease”, Biomarkers. 18(2).103-115. Mar 2013.
[49]  Goslin, R., O'Brien, M.J., Steele, G., Mayer, R., Wilson, R., Corson, J.M., Zamcheck, N., “Correlation of Plasma CEA and CEA tissue staining in poorly differentiated colorectal cancer”, Am J Med. 71(2). 246-53. Aug 1981.
[50]  Sarkar, T., Das, P., Mukhopadhyay, S., Chakraborty, A., Mukhopadhyay, A., "Correlation of carcinoembryonic antigen (CEA) with oxidativestress in carcinoma lung", Ann. Oncol. 26(suppl 9). 6-6. Dec 2015.
[51]  Exeter clinical laboratory international. "Blood science test: Carcinoembryonic antigen (CEA) ",