Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Go
Journal of Food and Nutrition Research. 2019, 7(11), 785-793
DOI: 10.12691/jfnr-7-11-5
Open AccessArticle

Histopathological Examination of the Effects of Oral Consumption of Various Doses of Propolis in Mice Liver

Fatemeh Samiee-Rad1, Sadaf Bagherkhani1 and Nematollahe Gheibi1,

1Department of Pathobiology, Metabolic Diseases Research Center, Research Institute for Non-communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran

Pub. Date: November 19, 2019

Cite this paper:
Fatemeh Samiee-Rad, Sadaf Bagherkhani and Nematollahe Gheibi. Histopathological Examination of the Effects of Oral Consumption of Various Doses of Propolis in Mice Liver. Journal of Food and Nutrition Research. 2019; 7(11):785-793. doi: 10.12691/jfnr-7-11-5

Abstract

Introduction: Considering the widespread application of biological substances, such as Propolis, it is essential to identify the importance of its liver metabolism, its effects of the liver and safe doses. The aim of this study was to determine the histopathological effects of oral administration of Propolis on the liver in mice. Methods and materials: This study consisted of five experimental groups of male mice (25-30 g), including control, placebo and 3 treatment groups. Male mice (25-30 g). Each of the groups receiving Propolis extract at one of the 2000, 4000 and 8000 mg/kg concentrations. Mice were gavage fed with the alcoholic extract of Iranian Propolis once daily for 14 days. After induction of a deep anesthesia, the liver was removed and examined for histopathological changes. All the data were analyzed using SPSS software (P <0.05). Results: The results of this study indicated that chronic administration of various doses of routine Propolis induced different histopathological changes in a dose-dependent manner. These changes initiated around the central venous; furthermore, their severity was higher in this part of the liver compare to the other areas. Conclusion: Based on the results of this study, it is essential to carefully monitor the dosage and consumption duration of Propolis. Moreover, periodical examination of liver function is essential during chronic consumption of Propolis.

Keywords:
Propolis mice liver histopathology

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  .Baruah MJ, Baruah PK, Borthakur MK. A Study of Histopathogical Examination of Alcohol Induced Hepato-Toxicity in Albino Rats and Protective Effect of Cuminum cyminum. International Journal of Science and Research (IJSR). 2016; 5(10): 693-699.
 
[2]  Weiskirchen R, Weiskirchen S, Tacke F. Recent advances in understanding liver fibrosis: bridging basic science and individualized treatment concepts. F1000Research 2018, 7(F1000 Faculty Rev): 921 Last updated: 15 AUG 2018.
 
[3]  Ishak KBaptista A, Callea F, De Groote J, Gudat F, and et al. Histopathological grading and staging of chronic hepatitis. J Hepatol. 1995; 22: 696-699.
 
[4]  Bankova V, Popova M, Trusheva B. Propolis volatile compounds: chemical diversity and biological activity: a review. Chemistry Central Journal. 2014; 8(1): 28.
 
[5]  Campos JF, dos Santos UP, Macorini LF, de Melo AM, Balestieri JB, Paredes-Gamero EJ, Cardoso CA, de Picoli Souza K, dos Santos EL. Antimicrobial, antioxidant and cytotoxic activities of propolis from Melipona orbignyi (Hymenoptera, Apidae). Food and Chemical Toxicology. 2014 Mar 1; 65: 374-80.
 
[6]  Cavendish RL, de Souza Santos J, Neto RB, Paixão AO, Oliveira JV, de Araujo ED, e Silva AA, Thomazzi SM, Cardoso JC, Gomes MZ. Antinociceptive and anti-inflammatory effects of Brazilian red propolis extract and formononetin in rodents. Journal of ethnopharmacology. 2015 Sep 15; 173: 127-33.
 
[7]  Ali BM, Ghoname NF, Hodeib AA, Elbadawy MA. Significance of topical propolis in the treatment of facial acne vulgaris. Egyptian Journal of Dermatology and Venerology. 2015 Jan 1; 35(1): 29.
 
[8]  Olczyk P, Komosinska-Vassev K, Wisowski G, Mencner L, Stojko J, Kozma EM. Propolis modulates fibronectin expression in the matrix of thermal injury. BioMed research international. 2014; 2014. Article ID 748101
 
[9]  Ivana K , Ivan M , Vatroslav S , Albina D , Jasna J , Maja M.The Effects of Propolis and Bee Pollen Supplementation on Biochemical Blood Parameters of Broilers. Acta Veterinaria-Beograd 2018, 68 (2), 190-200.
 
[10]  Li H, Liu Y , Zhang X , Xu Q , Zhang Y , Xue C and Guo C. Medium-chain fatty acids decrease serum cholesterol via reduction of intestinal bile acid reabsorption in C57BL/6J mice.Nutrition & Metabolism (2018) 15: 37.
 
[11]  Mujica V, Orrego R, Pérez J, Romero P, Paz Ovalle P, Zúñiga-HernándezJ, et al. The Role of Propolis in Oxidative Stress and Lipid Metabolism: A Randomized Controlled Trial. Evid Based Complement Alternat Med. 2017; 2017: 4272940.
 
[12]  Yuan X, Wang Y, Shi B, Zhao Y. Effect of propolis on preserving human periodontal ligament cells and regulating pro‐inflammatory cytokines. Dental traumatology. 2018, Volume 34, issue4: 245-253.
 
[13]  Rao Pasupuleti V, Sammugam L, Ramesh N, and Hua Gan S. Honey, Propolis, and Royal Jelly: A Comprehensive Review of Their Biological Actions and Health Benefits. Oxid Med Cell Longev. 2017; 2017: 1259510.
 
[14]  Wali AF, Avula B, Ali Z, Khan IA, Mushtaq A, Rehman MU, Akbar S, Masoodi MH. Antioxidant, hepatoprotective potential and chemical profiling of propolis ethanolic extract from Kashmir Himalaya region using UHPLC-DAD-QToF-MS. BioMed research international. 2015; 2015: 393462.
 
[15]  Martínez Pallás I, Conejero Del Mazo C,Prieto Torres L. Allergic contact stomatitis caused by propolis candies. Contact dermatitis, 2018, 78(6): 418-419.
 
[16]  Nauffal M, Gabardi S. Nephrotoxicity of Natural Products. Blood Purif 2016; 41: 123-129.
 
[17]  Regueira-Neto MDS , Tintino SR, Rolón M , Coronal C, Vega MC, de Queiroz Balbino V, de Melo Coutinho HD. Antitrypanosomal, antileishmanial and cytotoxic activities of Brazilian red propolis and plant resin of Dalbergia ecastaphyllum (L) Taub. Food Chem Toxicol. 2018 Sep; 119: 215-221.
 
[18]  Cruz M, Antunes P, Paulo L, Ferreira AM, Cunha A, Almeida-Aguiar C and Oliveira R.Antioxidant and dual dose-dependent antigenotoxic and genotoxic properties of an ethanol extract of propolis. RSC Adv., 2016, 6, 49806-49816.
 
[19]  Sforcin JM. Biological Properties and Therapeutic Applications of Propolis. Phytother. Res. 30: 894-905 (2016).
 
[20]  Ryu CS, Oh SJ, Oh JM, Lee J-Y, Lee SY, Chae J-w, et al. Inhibition of cytochrome P450 by propolis in human liver microsomes. 2016; 32(3): 207.
 
[21]  Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of ethnopharmacology. 2000 Jul 1; 71(1-2): 109-14.
 
[22]  Lorke D. A new approach to practical acute toxicity testing. Archives of toxicology. 1983 Dec 1; 54(4): 275-87.
 
[23]  Ramadan A, Soliman G, Mahmoud SS, Nofal SM, Abdel-Rahman RF. Evaluation of the safety and antioxidant activities of Crocus sativus and Propolis ethanolic extracts. Journal of Saudi Chemical Society. 2012 Jan 1; 16(1): 13-21.
 
[24]  Gheibi N, Shahbazi J, Zarmohammadi Z, Alipoor Heydari M, Kakaeie E, Sofiabadi M.Effect of Oral Administration of Ethanolic Extracts of Propolis onPassive Avoidance Learning and Memory in Adult Male Mice. Journal of Ardabil University of Medical Sciences. 2017; 17(1): 104-112.
 
[25]  Wagh VD. Propolis: a wonder bees product and its pharmacological potentials. Advances in pharmacological sciences. 2013; 2013. Article ID 308249.
 
[26]  Scandinari Manzolli E, Mara Serpeloni J, Grotto D, Kennup Bastos J, Maria Greggi Antunes L, Barbosa F, 1 and Rafael Mazzaron Barcelos G. Protective Effects of the Flavonoid Chrysin against Methylmercury-Induced Genotoxicity and Alterations of Antioxidant Status, In Vivo Oxid Med Cell Longev. 2015; 2015: 602360.
 
[27]  Bhadauria M. Propolis prevents hepatorenal injury induced by chronic exposure to carbon tetrachloride. Evidence-Based Complementary and Alternative Medicine. 2012; 2012. Article ID 235358.
 
[28]  Arvouet-Grand A, Lejeune B, Bastide P, Pourrat A, Privat AM, Legret P. Propolis extract. I. Acute toxicity and determination of acute primary cutaneous irritation index. Journal de pharmacie de Belgique. 1993; 48(3):165-70.
 
[29]  Mohammadzadeh S, Shariatpanahi M, Hamedi M, Ahmadkhaniha R, Samadi N, Ostad SN. Chemical composition, oral toxicity and antimicrobial activity of Iranian propolis. Food chemistry. 2007 Jan 1; 103(4): 1097-103.
 
[30]  Charlton M, Krishnan A, Viker K, Sanderson S, Cazanave S, McConico A, Masuoko H, Gores G. Fast food diet mouse: novel small animal model of NASH with ballooning, progressive fibrosis, and high physiological fidelity to the human condition. Am J Physiol Gastrointest Liver Physiol. 2011 Aug 11; 301(5): G825-34.
 
[31]  McGill MR, Williams CD, Xie Y, Ramachandran A, Jaeschke H. Acetaminophen-induced liver injury in rats and mice: comparison of protein adducts, mitochondrial dysfunction, and oxidative stress in the mechanism of toxicity. Toxicol Appl Pharmacol. 2012 Nov 1; 264(3): 387-94.
 
[32]  Najmi AK, Pillai KK, Pal SN, Akhtar M, Aqil M, Sharma M. Effect of l-ornithine l-aspartate against thioacetamide-induced hepatic damage in rats. Indian J Pharmacol. 2010 Dec; 42(6): 384.
 
[33]  McArthur K , T. Kile B. Apoptotic Caspases: Multiple or Mistaken Identities? Trends in Cell Biology, June 2018, Vol. 28, No. 6:475-493
 
[34]  Paschkowsky S, Hsiao JM, Young JC, Munter LM. The discovery of proteases and intramembrane proteolysis. Biochem Cell Biol. 2018 Aug 13:1-5.
 
[35]  Campbell KJ, Tait SWG.Targeting BCL-2 regulated apoptosis in cancer. Open Biol. 2018 May; 8(5): 180002.
 
[36]  Padda, M.S., M. Sanchez, A.J. Akhtar, and J.L. Boyer. Drug-induced cholestasis. Hepatology (Baltimore, Md.). 2011; 53: 1377-1387.
 
[37]  Guy CD, Suzuki A, Zdanowicz M, Abdelmalek MF, Burchette J, Unalp A, Diehl AM. Hedgehog pathway activation parallels histologic severity of injury and fibrosis in human nonalcoholic fatty liver disease. Hepatology. 2012 Jun 1; 55(6): 1711-21. Epub 2012 Apr 18.
 
[38]  Danielson PB. The cytochrome P450 superfamily: biochemistry, evolution and drug metabolism in humans. Current drug metabolism. 2002 Dec 1; 3(6): 561-97.
 
[39]  Jeffery, E.H.. Biochemical Basis of Toxicity. In Handbook of Toxicologic Pathology. W.M. Haschek and C.G. Rousseaux, editors. Academic Press Inc., London 1991. 49-70.
 
[40]  J. Felmlee D, Grün D, F. Baumert T. Zooming in on liver zonation. Hepatology 2018, volume 67, issue 2: 784-787.