Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2019, 7(3), 187-190
DOI: 10.12691/jfnr-7-3-2
Open AccessArticle

Free Radical Scavenging Activities and Neuro-protective Effects of Extracts from Juniperus rigida Sieb. Fruit

Hyun Woo Kang1, and Sang Jae Kwak1

1Department of Korean Food & Culinary Arts, Youngsan University, Busan, 48015, Korea

Pub. Date: March 02, 2019

Cite this paper:
Hyun Woo Kang and Sang Jae Kwak. Free Radical Scavenging Activities and Neuro-protective Effects of Extracts from Juniperus rigida Sieb. Fruit. Journal of Food and Nutrition Research. 2019; 7(3):187-190. doi: 10.12691/jfnr-7-3-2

Abstract

The antioxidative activity of extracts from Juniperus rigida Sieb. Fruit (JRSF) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. The free radical scavenging activity of the extract was dose dependant manner, and the IC50 value on DPPH, hydroxyl and alkyl radical at 16.84, 82.35 and 52.27 μg/mL respectively. In addition, evaluated the protection effect on H2O2-induced oxidative damage in PC-12 cells via propidium iodide (PI) staining using a flow cytometer. JRSF extract decreased cell death in PC-12 cells due to H2O2-induced oxidative damage in a dose-dependent manner. These results suggest that JRSF extract exhibit antioxidative activity against oxidative stress on PC-12 cells.

Keywords:
Juniperus rigida Sieb. fruit antioxidant free radical PC-12 cells flow cytometer

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