Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2019, 7(3), 187-190
DOI: 10.12691/jfnr-7-3-2
Open AccessArticle

Free Radical Scavenging Activities and Neuro-protective Effects of Extracts from Juniperus rigida Sieb. Fruit

Hyun Woo Kang1, and Sang Jae Kwak1

1Department of Korean Food & Culinary Arts, Youngsan University, Busan, 48015, Korea

Pub. Date: March 02, 2019

Cite this paper:
Hyun Woo Kang and Sang Jae Kwak. Free Radical Scavenging Activities and Neuro-protective Effects of Extracts from Juniperus rigida Sieb. Fruit. Journal of Food and Nutrition Research. 2019; 7(3):187-190. doi: 10.12691/jfnr-7-3-2


The antioxidative activity of extracts from Juniperus rigida Sieb. Fruit (JRSF) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. The free radical scavenging activity of the extract was dose dependant manner, and the IC50 value on DPPH, hydroxyl and alkyl radical at 16.84, 82.35 and 52.27 μg/mL respectively. In addition, evaluated the protection effect on H2O2-induced oxidative damage in PC-12 cells via propidium iodide (PI) staining using a flow cytometer. JRSF extract decreased cell death in PC-12 cells due to H2O2-induced oxidative damage in a dose-dependent manner. These results suggest that JRSF extract exhibit antioxidative activity against oxidative stress on PC-12 cells.

Juniperus rigida Sieb. fruit antioxidant free radical PC-12 cells flow cytometer

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[1]  Frlich, I., and Riederer, P., “Free radical mechanisms in dementia of Alzheimer type and the potential for antioxidative treatment” Drug Research 45, 443-449. 1995.
[2]  Halliwell, B., and Gutterridge, J.M.C., “Free radicals in biology and medicine (2nd ed.). Oxford: Clarendon Press”, 1989.
[3]  Kim, S.K., Kim, Y.T., Byun, H.G., Park, P.J., and Ito, H., “Purification and characterization of antioxidative peptides from bovine skin” J Biochem Mol Biol 34, 219-224. 2001.
[4]  Wanita, A., and Lorenz, K., “Antioxidant potential of 5-Npentadecylresorcinol” J Food Process Preserv 20, 417-429. 1996.
[5]  Adams, R.P., Chu, G., and Zhang, S., “Comparisons of the volatile leaf oils of Juni-perus rigida Mig. From north eastern China, Korea and Japan” J Essent Oil Res 7, 49-52. 1995.
[6]  Lee, Y., Kim, J., Kim, H., Sohn, E., Kim, C., Jeong, I., Jo, K., Kim, J., and Kim, J., “Anti-lipase and lipolytic activities of EtOH extract from Juniperus rigida” Korean J Pharm 41, 216-220. 2010.
[7]  Woo, K.W., Choi, S.U., Park, J.C., and Lee, K.R., “A new lignin glycoside from Juni-perus rigida” Arch Pharm Res 34, 2043-2049. 2011.
[8]  Jeong, E., Seo, H., Yang, H., Kim, J., Sung, S., and Kim, Y., “Anti-inflammatory phe- nolics isolated from Juniperus rigida leaves and twigs in lipopolysaccharide- stimulated RAW264.7 macrophage cells” J Enzym Inhib Med Chem 27, 875-879. 2012.
[9]  Nanjo, F., Goto, K., Seto, R., Suzuki, M., Sakai, M., and Hara, Y., “Scavenging effects of tea catechins and their derivatives on 1,1,-diphenyl-2-picrylydrazyl radical” Free Rad Bio Med 21, 895-902. 1996.
[10]  Rosen, G.M., and Rauckman, E.J., “Spin trapping of superoxide and hydroxyl radicals” In Methods in Enzymology Packer, L., Ed.; Academic Press: Orlando, FL, Vol. 105, 198-209. 1984.
[11]  Lee, S.J., Kim, E.K., Oh, H.J., Kwon, H.J., Hwang, J.W., Moon, S.H., and Park P.J., “Free radical scavenging activity and protective effect against H2O2-induced stress in neuronal cells of enzymatic extracts from Sarcodon aspratus” Korean J Med Crop Sci 19(2), 77-82. 2011.
[12]  Lee, S.J., Kim, Y.S., Hwang, J.W., Kim, E.K., Moon, S.H., Jeon, B.T., Jeon, Y.J., Kim, J.M., and Park, P.J., “Purification and characterization of a novel antioxidative peptide from duck skin by-produc ts that protects liver against oxidative damage”, Food Res Int 49 (1). 285-295. 2012.
[13]  Hofseth, L.J., and Ying, L., “Identifying and defusing weapons of mass inflammation in carcinogenesis” Biochim Biophys Acta 1765, 74-84. 2006.
[14]  Hussain, S.P., Hofseth, L.J., and Harris, C.C., “Radical causes of cancer” Nat Rev Cancer 3, 276-285. 2003.