Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Go
Journal of Food and Nutrition Research. 2019, 7(2), 132-140
DOI: 10.12691/jfnr-7-2-5
Open AccessArticle

Araçá (Psidium cattleianum Sabine) Ameliorates Liver Damage and Reduces Hepatic Steatosis in Rats Fed with a High-fat Diet

Alice Helena S. PAULINO1, 2, , Ana Maria F. VIANA1, 2, Larissa F. BONOMO3, Joyce Ferreira C. GUERRA4, Juliana Márcia M. LOPES1, 2, Ana Carolina S. RABELO1, Miliane Martins A. FAGUNDES2, 5, Ana Lúcia Rissoni S. RÉGIS2, Wanderson G. LIMA1, 6, Maria Lúcia PEDROSA1, 6 and Marcelo Eustáquio SILVA1, 7

1Research Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, Ouro Preto, MG, Brazil

2School of Nutrition, Federal University of Ouro Preto, Ouro Preto, MG, Brazil

3Department of Pharmacy. Federal University of Juiz de Fora, Governador Valadares Campus, Governador Valadares, MG, Brazil

4Institute of Genetics and Biochemistry, Federal University of Uberlândia, Patos de Minas, MG, Brazil

5Department of Foods (DEALI), Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil

6Department of Biological Sciences (DECBI), Federal University of Ouro Preto, Ouro Preto, Brazil

7School of Nutrition, Federal University of Ouro Preto, Ouro Preto, MG, Brazil;Department of Foods (DEALI), Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil

Pub. Date: February 14, 2019

Cite this paper:
Alice Helena S. PAULINO, Ana Maria F. VIANA, Larissa F. BONOMO, Joyce Ferreira C. GUERRA, Juliana Márcia M. LOPES, Ana Carolina S. RABELO, Miliane Martins A. FAGUNDES, Ana Lúcia Rissoni S. RÉGIS, Wanderson G. LIMA, Maria Lúcia PEDROSA and Marcelo Eustáquio SILVA. Araçá (Psidium cattleianum Sabine) Ameliorates Liver Damage and Reduces Hepatic Steatosis in Rats Fed with a High-fat Diet. Journal of Food and Nutrition Research. 2019; 7(2):132-140. doi: 10.12691/jfnr-7-2-5

Abstract

Bioactive compounds, present in some foods, act enhancing the endogenous antioxidant system and are proposed as an effective strategy in preventing the changes induced by free radicals in some diseases, such as nonalcoholic fatty liver disease (NAFLD). There has been an increase in the number of studies carried out with the aim of finding natural antioxidant compounds present in fruits, mainly the native fruits of Brazil, because they contain a high content of these compounds. Araçá (Psidium cattleianum Sabine) is a fruit that is rich in polyphenols and exhibits strong antioxidant, antiproliferative, and anti-inflammatory activities. Therefore, the present study investigated the effects of araçá flour on oxidative stress, liver injury, and antioxidant defenses in high-fat diet-induced hepatic steatosis in rats. In vitro experiments showed that araçá contains high concentrations of total polyphenols and exhibits strong antioxidant activity with no cytotoxicity. In vivo experiments indicated that the addition of araçá to a high-fat diet inhibited the activities of alanine aminotransferase and aspartate enzymes, reduced macrovesicular steatosis, increased the paraoxonase activity, and increased the concentration of the total and reduced forms of glutathione. Therefore, our findings suggested the hepatoprotective role of araçá against the progression of steatosis.

Keywords:
Psidium cattleianum Sabine araçá phenolic compounds hepatic steatosis antioxidants defenses

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Figures

Figure of 3

References:

[1]  M. Vacca, M. Allison, J. L. Griffin, and A. Vidal-Puig, “Fatty Acid and Glucose Sensors in Hepatic Lipid Metabolism: Implications in NAFLD,” Semin Liver Dis, vol. 35, no. 3, pp. 250-261, 2015.
 
[2]  I. Nalbantoglu and E. M. Brunt, “Role of liver biopsy in nonalcoholic fatty liver disease,” World J. Gastroenterol., vol. 20, no. 27, pp. 9026-9037, 2014.
 
[3]  C. E. Foulds, L. S. Treviño, B. York, and C. L. Walker, “Endocrine-disrupting chemicals and fatty liver disease,” Nat. Rev. Endocrinol., vol. 13, no. 8, pp. 445-457, 2017.
 
[4]  R. Patell, “Non Alcoholic Fatty Liver Disease in Obesity,” J. Clin. Diagnostic Res., vol. 8, no. 1, pp. 62-66, 2014.
 
[5]  G. Serviddio, F. Bellanti, and G. Vendemiale, “Free radical biology for medicine: Learning from nonalcoholic fatty liver disease,” Free Radic. Biol. Med., vol. 65, pp. 952-968, 2013.
 
[6]  Y. Y. Chang et al., “Preventive effects of taurine on development of hepatic steatosis induced by a high-fat/cholesterol dietary habit,” J. Agric. Food Chem., vol. 59, no. 1, pp. 450-457, 2011.
 
[7]  M. de L. P. Bianchi and L. M. G. Antunes, “Radicais livres e os principais antioxidantes da dieta,” Rev. Nutr., vol. 12, no. 2, pp. 123-130, 1999.
 
[8]  M. Hariri et al., “Intakes of Vegetables and Fruits are Negatively Correlated with Risk of Stroke in Iran.,” Int. J. Prev. Med., vol. 4, no. Suppl 2, pp. S300-5, May 2013.
 
[9]  V. Lobo, A. Patil, A. Phatak, and N. Chandra, “Free radicals, antioxidants and functional foods: Impact on human health.,” Pharmacogn. Rev., vol. 4, no. 8, pp. 118-26, Jul. 2010.
 
[10]  Y. Liu et al., “Inhibitory effect of blueberry polyphenolic compounds on oleic acid-induced hepatic steatosis in vitro,” J. Agric. Food Chem., vol. 59, no. 22, pp. 12254-12263, 2011.
 
[11]  S.-J. Cho, U. J. Jung, and M.-S. Choi, “Differential effects of low-dose resveratrol on adiposity and hepatic steatosis in diet-induced obese mice,” Br. J. Nutr., vol. 108, no. 12, pp. 2166-2175, 2012.
 
[12]  J. F. da C. Guerra et al., “Dietary açai attenuates hepatic steatosis via adiponectin-mediated effects on lipid metabolism in high-fat diet mice,” J. Funct. Foods, vol. 14, pp. 192-202, 2015.
 
[13]  A. L. Medina et al., “Araçá (Psidium cattleianum Sabine) fruit extracts with antioxidant and antimicrobial activities and antiproliferative effect on human cancer cells,” Food Chem., vol. 128, no. 4, pp. 916-922, 2011.
 
[14]  A. B. Ribeiro, R. C. Chisté, M. Freitas, A. F. Da Silva, J. V. Visentainer, and E. Fernandes, “Psidium cattleianum fruit extracts are efficient in vitro scavengers of physiologically relevant reactive oxygen and nitrogen species,” Food Chem., vol. 165, pp. 140-148, 2014.
 
[15]  W. Horwitz, P. Chichilo, and H. Reynolds, “Official methods of analysis of the Association of Official Analytical Chemists.,” Off. methods Anal. Assoc. Off. Anal. Chem., 1970.
 
[16]  P. J. Van Soest, R. H. Wine, A. Husbandry, and R. División, “Use of Detergents in the Analysis of Fibrous Feeds. IV. Determination of Plant Cell-Wall Constituents.”
 
[17]  S. Georgé, P. Brat, P. Alter, and M. J. Amiot, “Rapid determination of polyphenols and vitamin C in plant-derived products,” J. Agric. Food Chem., vol. 53, no. 5, pp. 1370-1373, 2005.
 
[18]  W. Brand-Williams, M. E. Cuvelier, and C. Berset, “Use of a free radical method to evaluate antioxidant activity,” LWT - Food Sci. Technol., vol. 28, no. 1, pp. 25-30, 1995.
 
[19]  T. Mosmann, “Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays,” J. Immunol. Methods, vol. 65, no. 1-2, pp. 55-63, Dec. 1983.
 
[20]  P. G. Reeves, F. H. Nielsen, and G. C. Fahey, “AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet,” J. Nutr., vol. 123, no. 11, pp. 1939-1951, 1993.
 
[21]  S. L. Matos et al., “Dietary models for inducing hypercholesterolemia in rats,” Brazilian Arch. Biol. Technol., vol. 48, no. 2, pp. 203-209, 2005.
 
[22]  J. A. Buege and S. D. Aust, “Microsomal lipid peroxidation,” Methods Enzymol., vol. 52, pp. 302-310, Jan. 1978.
 
[23]  R. L. Levine et al., “Determination of carbonyl content in oxidatively modified proteins,” Methods Enzymol., vol. 186, pp. 464-478, Jan. 1990.
 
[24]  H. Aebi, “Catalase in vitro,” Methods Enzymol., vol. 105, pp. 121-126, Jan. 1984.
 
[25]  R. J. Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, “The folin by oliver,” Anal. Biochem., vol. 217, no. 2, pp. 220-230, 1951.
 
[26]  S. Marklund and G. Marklund, “Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase,” 1974.
 
[27]  O. W. Griffith, “Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine,” Anal. Biochem., vol. 106, no. 1, pp. 207-212, Jul. 1980.
 
[28]  J. Beltowski, G. Wojcicka, and A. Jamroz, “Differential effect of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors on plasma paraoxonase 1 activity in the rat 9,” Pol.J.Pharmacol., vol. 54, no. 1230-6002 (Print), pp. 661-671, 2002.
 
[29]  E. Albano et al., “Immune response towards lipid peroxidation products as a predictor of progression of non-alcoholic fatty liver disease to advanced fibrosis,” Gut, vol. 54, no. 7, pp. 987-993, 2005.
 
[30]  A. de Lima, A. M. de O. e Silva, R. A. Trindade, R. P. Torres, and J. Mancini-Filho, “Composição química e compostos bioativos presentes na polpa e na amêndoa do pequi (Caryocar brasiliense, Camb.),” Rev. Bras. Frutic., vol. 29, no. 3, pp. 695-698, 2007.
 
[31]  W. S. Rocha, R. M. Lopes, D. B. da Silva, R. F. Vieira, J. P. da Silva, and T. da S. Agostini-Costa, “Compostos fenólicos totais e taninos condensados em frutas nativas do cerrado,” Rev. Bras. Frutic., vol. 33, no. 4, pp. 1215-1221, 2011.
 
[32]  E. M. Kuskoski, A. G. Asuero, A. M. Troncoso, J. Mancini-Filho, and R. Fett, “Aplicación de diversos métodos químicos para determinar actividad antioxidante en pulpa de frutos,” Ciência e Tecnol. Aliment., vol. 25, no. 4, pp. 726-732, 2005.
 
[33]  A. Luísa, K. Faller, and E. Fialho, “Disponibilidade de polifenóis em frutas e hortaliças consumidas no Brasil Polyphenol availability in fruits and vegetables consumed in Brazil,” Rev Saúde Pública, vol. 43, no. 2, pp. 211-8, 2009.
 
[34]  G. A. B. Canuto, A. A. O. Xavier, L. C. Neves, and M. de T. Benassi, “Caracterização físico-química de polpas de frutos da Amazônia e sua correlação com a atividade anti-radical livre,” Rev. Bras. Frutic., vol. 32, no. 4, pp. 1196-1205, 2010.
 
[35]  H. Zhang and R. Tsao, “Dietary polyphenols, oxidative stress and antioxidant and anti-inflammatory effects,” Curr. Opin. Food Sci., vol. 8, pp. 33-42, 2016.
 
[36]  M. Guasch-Ferré, J. Merino, Q. Sun, M. Fitó, and J. Salas-Salvadó, “Dietary Polyphenols, Mediterranean Diet, Prediabetes, and Type 2 Diabetes: A Narrative Review of the Evidence,” Oxid. Med. Cell. Longev., vol. 2017, 2017.
 
[37]  K. S. Bhullar and H. P. V. Rupasinghe, “Polyphenols: Multipotent Therapeutic Agents in Neurodegenerative Diseases, Polyphenols: Multipotent Therapeutic Agents in Neurodegenerative Diseases,” Oxidative Med. Cell. Longevity, Oxidative Med. Cell. Longev., vol. 2013, 2013, p. e891748, 2013.
 
[38]  M. C. Pereira et al., “Characterization and antioxidant potential of Brazilian fruits from the Myrtaceae family,” J. Agric. Food Chem., vol. 60, no. 12, pp. 3061-3067, 2012.
 
[39]  O. A. Esmael, S. N. Sonbul, T. A. Kumosani, and S. . Moselhy, “Hypolipidemic effect of fruit fibers in rats fed with high dietary fat,” Toxicol. Ind. Health, vol. 31, no. 3, pp. 281-288, Mar. 2015.
 
[40]  Y. E. Pérez-Beltrán et al., “Nutritional characteristics and bioactive compound content of guava purees and their effect on biochemical markers of hyperglycemic and hypercholesterolemic rats,” J. Funct. Foods, vol. 35, pp. 447-457, Aug. 2017.
 
[41]  T. Trasher and M. Abelmalek, “Non-alcoholic fatty liver disease Non-alcoholic fatty liver disease,” N C Med J, vol. 50, no. August, pp. 1-5, 2016.
 
[42]  H. Yoshitomi, X. Guo, T. Liu, and M. Gao, “Guava leaf extracts alleviate fatty liver via expression of adiponectin receptors in SHRSP.Z-Leprfa/Izm rats,” Nutr. Metab., vol. 9, no. 1, p. 13, 2012.
 
[43]  M. Carmiel-Haggai, A. I. Cederbaum, and N. Nieto, “A high-fat diet leads to the progression of non-alcoholic fatty liver disease in obese rats.,” FASEB J., vol. 19, no. 1, pp. 136-138, 2005.
 
[44]  W. C. Chiu, H. H. Yang, S. C. Chiang, Y. X. Chou, and H. T. Yang, “Auricularia polytricha aqueous extract supplementation decreases hepatic lipid accumulation and improves antioxidative status in animal model of nonalcoholic fatty liver,” Biomed., vol. 4, no. 2, pp. 29-38, 2014.
 
[45]  D. Dong et al., “Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats,” Food Funct., vol. 5, no. 12, pp. 3065-3075, 2014.
 
[46]  A. M. Teixeira, F. C. Chaves, and C. V. Franzon, Rodrigo Cezar, Rombaldi, “Influence of Genotype and Harvest Season on the Phytochemical Composition of Araçá (Psidium Cattleianum Sabine) Fruit,” Int. J. Food Nutr. Sci., vol. 3, no. 4, pp. 1-7, 2016.
 
[47]  M. Aviram and M. Rosenblat, “Paraoxonases 1, 2, and 3, oxidative stress, and macrophage foam cell formation during atherosclerosis development,” Free Radic. Biol. Med., vol. 37, no. 9, pp. 1304-1316, 2004.
 
[48]  M. Aviram et al., “Pomegranate juice consumption reduces oxidative stress and low density lipoprotein atherogenic modifications: studies in the atherosclerotic apolipoprotein E deficient mice and in humans,” Am. J. Clin. Nutr., vol. 151, p. 111, 2000.
 
[49]  R. R. Pereira et al., “A�ai (Euterpe oleracea Mart.) Upregulates Paraoxonase 1 Gene Expression and Activity with Concomitant Reduction of Hepatic Steatosis in High-Fat Diet-Fed Rats,” Oxid. Med. Cell. Longev., vol. 2016, 2016.
 
[50]  P. C. Huber, W. P. Almeida, and Â. De Fátima, “Glutationa e enzimas relacionadas: Papel biologico e importancia em processos patologicos,” Quim. Nova, vol. 31, no. 5, pp. 1170-1179, 2008.
 
[51]  L. Yuan and N. Kaplowitz, “Glutathione in liver diseases and hepatotoxicity,” Mol. Aspects Med., vol. 30, no. 1-2, pp. 29-41, Feb. 2009.