Journal of Environment Pollution and Human Health
ISSN (Print): 2334-3397 ISSN (Online): 2334-3494 Website: Editor-in-chief: Dibyendu Banerjee
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Journal of Environment Pollution and Human Health. 2018, 6(1), 31-37
DOI: 10.12691/jephh-6-1-5
Open AccessArticle

Occurrence of Pathogenic Bacteria Harbouring Antibiotic Resistant Genes in River Njoro in Nakuru County, Kenya

Itotia T. K.1, , Muia A. W.2, Kiruki S.K3 and Getenga Z.3

1Departmment of Biochemistry, Egerton University, Nakuru, Kenya

2Department of Biological Science, Egerton University, Nakuru, Kenya

3Department of Physical Science, Chuka University, Chuka, Kenya

Pub. Date: February 08, 2018

Cite this paper:
Itotia T. K., Muia A. W., Kiruki S.K and Getenga Z.. Occurrence of Pathogenic Bacteria Harbouring Antibiotic Resistant Genes in River Njoro in Nakuru County, Kenya. Journal of Environment Pollution and Human Health. 2018; 6(1):31-37. doi: 10.12691/jephh-6-1-5


Njoro River drains an agricultural catchment whose main livelihood activities are livestock rearing and employment in light industries. The limited quantities of piped water supplies coupled with inadequate sanitary facilities experienced in the area can contribute to the spread of antibiotic resistance from antibiotic use in agriculture and human. The objective of the current study was to isolate common waterborne enteric pathogens and test them for antibiotic resistance on some commonly used antibiotics. The organisms that were found to be resistant were also tested for the presence resistant genes. This was done by filtering known quantities of water through membrane filters and plating them on selective and differential media and these were tested for sensitivity to antibiotics. Isolates that showed antibiotic resistance were tested for the presence of tetracycline (Tet A), Sulfamethoxazole (Sul2) genes class 1 intergrase gene and SXT element resistance genes using PCR with appropriate primers. Pathogens including E. coli strains, Salmonella spp, Vibrio cholera and V. parahaemolyticus were recorded in this study. Sulfamethoxazole (Sul2) genes were detected in Klebsiella pneumonia, Klebsiella oxytoca isolates and Entero- aggregative strains of E. coli. Tetracycline (tet A) genes were detected in ETEC and EAEC pathogenic strains of E.coli. Class 1 integrase was detected in an EAEC strain. The SXT (int) element was not detected in any of the isolates tested.

antibiotics pathogens resistance resistance genes

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[1]  Le Loir, Y., Baron, F., and Gautier, M. (2003). Staphylococcus aureus and food poisoning. Genetic Molecule Resistance, 2(1), 63-76.
[2]  Farmer, P., Almazor, C. P., Bahnsen, E. T., Barry, D., Bazile, J., Bloom, B. R., and Cravioto, A. (2003). Meeting cholera's challenge to Haiti and the world: a joint statement on cholera prevention and care. PLoSNegl Trop Dis, 5(5), 1145.
[3]  Strockbine, N. A. ,Brooks, J. T., Sowers, E. G., Wells, J. G., Greene, K. D., Griffin, P. M., Hoekstra, R. M., and (2005). Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002.Journal of Infectious Diseases, 192(8), 1422-1429.
[4]  Bettelheim, K. A., and Beutin, L. (2003). Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC). Journal of Applied Microbiology, 95(2), 205-217.
[5]  Ashbolt, N. J. (2004). Microbial contamination of drinking water and disease outcomes in developing regions. Toxicology, 198(1), 229-238.
[6]  Handfield, M., Simard, P., Couillard, M., and Letarte, R. (1996).Aeromonashydrophila isolated from food and drinking water: hemagglutination, hemolysis, and cytotoxicity for a human intestinal cell line (HT-29). Applied and Environmental Microbiology, 62(9), 3459.
[7]  Alonso, A., Sanchez, P., and Martinez, J. L. (2001). Environmental selection of antibiotic resistance genes. Environmental Microbiology, 3(1), 1-9.
[8]  Rowe-Magnus, D. A., and Mazel, D. (2002). The role of integrons in antibiotic resistance gene capture. International Journal of Medical Microbiology, 292(2), 115-125.
[9]  Fluit, A. C., and Schmitz, F. J. (2004).Resistance integrons and super-integrons.Clinical Microbiology and Infection, 10(4), 272-288.
[10]  Recchia, G. D., and Hall, R. M. (1997). Origins of the mobile gene cassettes found in integrons. Trends in microbiology, 5(10), 389-394.
[11]  Kazama, H., Kizu, K., Iwasaki, M., Hamashima, H., Sasatsu, M. and Arai, T. (1995).Isolation and structure of a new integron that includes a streptomycin resistance gene from the R plasmid of Pseudomonas aeruginosa. FEMS microbiology letters, 134 (2-3), 137-141.
[12]  Threlfall, E. J., Ward, L. R., Skinner, J. A., and Rowe, B. (1997). Increase in multiple antibiotic resistance in nontyphoidal salmonellas from humans in England and Wales: a comparison of data for 1994 and 1996. Microbial Drug Resistance, 3(3), 263-266.
[13]  Zhanel, G. G., Homenuik, K., Nichol, K., Noreddin, A., Vercaigne, L., Embil, J. and Hoban, D. J. (2004).The glycylcyclines. Drugs, 64(1), 63-88.
[14]  Chopra, I., and Roberts, M. (2001). Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiology and molecular biology reviews, 65(2), 232-260.
[15]  Chemelil, M. C. (1995). The Effects of Human-induced Watershed Changes on Stream Flows (Doctoral dissertation, © MC Chemelil).
[16]  Yillia, P. T., Kreuzinger, N., and Mathooko, J. M. (2009). The effect of in-stream activities on the Njoro River, Kenya. Part II: Microbial water quality. Physics and Chemistry of the Earth, Parts A/B/C, 33(8), 729-737.
[17]  Kobayashi, T., Suehiro, F., CachTuyen, B., and Suzuki, S. (2007). Distribution and diversity of tetracycline resistance genes encoding ribosomal protection proteins in Mekong River sediments in Vietnam.FEMS Microbiology Ecology, 59(3), 729-737.
[18]  Mokaya, S. K., Mathooko, J. M., and Leichtfried, M. (2004). Influence of anthropogenic activities on water quality of a tropical stream ecosystem. African Journal of Ecology, 42(4), 281-288.
[19]  Omigie, O., Tatfeng, Y., Omorogbe, F. I., Aisabokhale, F., and Ugbodagah, O. P. (2006). Antimicrobial susceptibility and plasmid profiles of Escherichia coli isolates obtained from different human clinical specimens in Lagos-Nigeria. Journal of Animal Science, 2, 70-76.
[20]  Roberts, M. C. (2005). Update on acquired tetracycline resistance genes. FEMS microbiology letters, 245(2), 195-203.
[21]  Martin, J. N., Rose, D. A., Hadley, W. K., Perdreau-Remington, F., Lam, P. K., and Gerberding, J. L. (1999). Emergence of trimethoprim-sulfamethoxazole resistance in the AIDS era. Journal of Infectious Diseases, 180(6), 1809-1818.
[22]  Hochhut, B., Lotfi, Y., Mazel, D., Faruque, S. M., Woodgate, R., and Waldor, M. K. (2001). Molecular analysis of antibiotic resistance gene clusters in Vibrio cholerae O139 and O1 SXT constins. Antimicrobial agents and chemotherapy, 45 (11), 2991-3000.
[23]  Yamai, S., Okitsu, T., Shimada, T., and Katsube, Y. (1997). [Distribution of serogroups of Vibrio cholerae non-O1 non-O139 with specific reference to their ability to produce cholera toxin, and addition of novel serogroups]. Kansenshogakuzasshi. The Journal of the Japanese Association for Infectious Diseases, 71(10), 1037-1045.