Journal of Cancer Research and Treatment
ISSN (Print): 2374-1996 ISSN (Online): 2374-2003 Website: http://www.sciepub.com/journal/jcrt Editor-in-chief: Jean Rommelaere
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Journal of Cancer Research and Treatment. 2014, 2(3), 64-68
DOI: 10.12691/jcrt-2-3-4
Open AccessArticle

Abrogation by Curcumin on Testicular Toxicity Induced by Cisplatin in Rats

Ehab Tousson1, , Ezar Hafez1, Ahmed Masoud1 and Aya A. Hassan1

1Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt

Pub. Date: November 19, 2014

Cite this paper:
Ehab Tousson, Ezar Hafez, Ahmed Masoud and Aya A. Hassan. Abrogation by Curcumin on Testicular Toxicity Induced by Cisplatin in Rats. Journal of Cancer Research and Treatment. 2014; 2(3):64-68. doi: 10.12691/jcrt-2-3-4

Abstract

Cisplatin is one of the most effective and widely-used antineoplastic agents for the treatment of testicular germ cell tumors. The present study was conducted to examine the possible modifying effects of curcumin against testicular toxicity induced by cisplatin in male rats. 60 male albino rats were equally divided into six groups; the first and second groups were the control and curcumin treated group respectively while the 3rd group was cisplatin treated group; the 4th and 5th groups were co- and post treated cisplatin rat with curcumin respectively and the 6th group was self treated cisplatin rat group. Many side effects were observed in animals injected with cisplatin such as loosing of body weight, loss of activity, weakness, yellowish body hair. A significant decrease in the body and testicular weights,, sperm counts, sperm motility, plasma testosterone, luteinizing hormone reduced glutathione, total antioxidant capacity, and total protein levels in cisplatin and cisplatin self treated groups when compared with the control group. On the other hand; a significant increase in the MDA and NO in cisplatin and cisplatin self treated groups when compared with the control group. sperm count and sperm motility, GSH and TAC exhibited significant increased in cisplatin treated with curcumin when compared with cisplatin groups, moreover, sperm abnormality, MDA, NO and total protein exhibited significant decrease in cisplatin treated groups with curcumin when compared with cisplatin groups. A significant decrease in sperm abnormality, MDA, NO and total protein and a significant increase in GSH and TAC in Co-treated group when compared with post treated cisplatin with curcumin. Our recommendation, administration of curcumin caused ameliorative effect against cisplatin-induced testicular toxicity.

Keywords:
cisplatin curcumin testis reproductive hormones oxidative stress

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References:

[1]  Boulikas T, Vougiouka M. Cisplatin and platinum drugs at the molecular level. Review Oncol Rep 2003; 10 (6): 1663-1682.
 
[2]  Kelland, L. The resurgence of platinum-based cancer chemotherapy. Nat Rev Cancer 2007; 7 (8): 573-584.
 
[3]  Galluzzi L, Senovilla L, Vitale I, Michels J, Martins I, Kepp O, Castedo M, Kroemer G. Molecular mechanisms of cisplatin resistance. Oncogene 2012; 31 (15): 1869-1883.
 
[4]  Cohen SM, Lippard SJ. Cisplatin: from DNA damage to cancer chemotherapy. Prog Nucleic Acid Res 2001; 67: 93-130.
 
[5]  Victoria Cepeda, Miguel A. Fuertes, Josefina Castilla, Carlos Alonso, Celia Quevedo and Jose M. Pérez Biochemical Mechanisms of Cisplatin Cytotoxicity. Anticancer Agents Med Chem 2007; 7 (1): 3-18.
 
[6]  Jordan P, Carmo-Fonseca M. Molecular mechanisms involved in cisplatin cytotoxicity. Cell Mol Life Sc 2000; 57 (8-9): 1229-1235.
 
[7]  Aggarwal BB, Sung B. Pharmacological basis for the role of curcumin in chronic diseases: an age-old spice with modern targets. Trends Pharmacol Sci 2009; 30 (2): 85-94.
 
[8]  Ilbey YO, Ozbek E, Simsek A, Otunctemur A, Cekmen M, Somay A. Potential chemoprotective effect of melatonin in cyclophosphamide and cisplatin-induced testicular damage inrats. Fertil Steril 2009; 92 (3): 1124-1132.
 
[9]  Noorafshan A, Karbalay-Doust S, Valizadeh A, Aliabadi E. Ameliorative effects of curcumin on the structural parameters of seminiferous tubules and Leydig cells in metronidazole-treated mice: a stereological approach. Exp Toxicol Pathol 2011; 63 (7): 627-633.
 
[10]  Khorsandi L, Mirhoseini M, Mohamadpour M, Orazizadeh M, Khaghani S. effect of curcumin on dexamethasone-induced testicular toxicity in mice. Pharm Biol 2013; 51 (2): 206-212.
 
[11]  Payton F, Sandusky P, Alworth WL, RMN Study of the SolutionStructure of Curcumin. J Nat Prod 2007; 70 (2): 144-146.
 
[12]  Goel A, Jhurani S, Aggarwal BB. Multi-targeted therapy by curcumin: how spicy is it? J Nat Prod 2008; 70 (2): 143-146.
 
[13]  Choudhary D, Chandra D, Kale RK. Modulation of radioresponse of glyoxalase system by curcumin. J Ethnopharmacol 1999; 64 (1): 1-7.
 
[14]  Sanchez-Gonzalez PD, Lopez-Hernandez FJ, Perez-Barriocanal F, Morales AI, Lopez-Novoa JM. Quercetin reduces cisplatin nephrotoxicity in rats without compromising its anti-tumour activity; Nephrol Dial Transplant 2011; 26 (11): 3484-3495.
 
[15]  Seed J., Chapin R.E., Clegg E.D., Dostal L.A., Foote R.H., Hurtt M.E., Klinefelter G.R., Makris S.L., Perreaultys S.D., Schrader S., Seyler D., Sparando R, Treine KA, Veeramacheneni DNR, Wise LD. Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: a consensus report. Reprod Toxicol 1996; 10 (3): 237-244
 
[16]  Cheng D, Zheng X M, Li S W, Yang Z W and Hu L Q. Effects of epidermal growth factor on sperm content and motility of rats with surgically induced varicoceles. Asian J Androl 2006; 8 (6): 713-717.
 
[17]  Abraham GE, Manlimos FS, Garza R. Radioimmunoassay of steroids. Chap. 20 in Handbook of Radioimmunoassay, GE Abraham, Ed., Marcel Dekker, Inc., New York, NY, 1977, pp 591-656
 
[18]  Taylor, A.E., McCourt, B., Martin, K.A., Anderson EJ, Adams JM, Schoenfeld D, Hall JE. Determinants of abnormal gonadotropin secretion in clinically defined women with polycystic ovary syndrome. J Clin Endocrinol Metab 1997;82, 2248-2256.
 
[19]  Koracevic, JD, Koracevic G, Djordjevic V, Andrejevic S. and Cosic V. Method for the measurement of antioxidant activity in human fluids. Clin Pathol 2001; 54: 356-361.
 
[20]  Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
 
[21]  Satoh, K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta 1978; 90: 37-43.
 
[22]  Beutler E, Duron O, and Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882-888.
 
[23]  Montgomery HAC, Dymock JF. The determination of nitrate in water. Analyst 1961; 86: 414-416.
 
[24]  Rebillard A, Lagadic-Gossmann D, Dimanche-Boitrel MT Cisplatin cytotoxicity: DNA and plasma membrane targets. Curr Med Chem 2008; 15 (26): 2656-2663.
 
[25]  Park GY, Wilson JJ, Song Y, Lippard SJ. Phenanthriplatin, a monofunctional DNA-binding platinum anticancer drug candidate with unusual potency and cellular activity profile. Proc Natl Acad Sci U S A 2012; 109 (30): 11987-11992.
 
[26]  Sadowitz PD, Hubbard BA, Dabrowiak JC, Goodisman J, Tacka KA, Aktas MK, Cunningham MJ, Dubowy RL, and Souid A-K. Kinetics of cisplatin binding to cellular DNA and modulations by thiol-blocking agents and thiol drugs. Drug Metab Dispos 2002; 30: 183-190
 
[27]  Shirwaikar A, Issac D, Malini S Effect of Aerva lanata on cisplatin and gentamicin models of acute renal failure. J Ethnopharmacol 2004; 90 (1): 81-86.
 
[28]  Helmy MW, Helmy MM, Abd Allah DM, Abo Zaid AM, Mohy El-Din MM. Role of nitrergic and endothelin pathways modulations in cisplatin-induced nephrotoxicity in male rats. J Physiol Pharmacol 2014; 65 (3): 393-399.
 
[29]  Turk G, Atessahin A, Sçnmez M, Ceribasi AO, YuceA. Improvement of cisplatin-induced injuries to sperm quality, the oxidant-antioxidant system, and the histologic structure of the rattestis by ellagic acid. Fertil Steril 2008; 89 (5): 1474-1481
 
[30]  Beytur A, Ciftci O, Oguz F, Oguzturk H, Yilmaz F. Montelukast attenuates side effects of cisplatin including testiculars morphologica and hormonal damage in male rats. Cancer Chemother Pharmacol 2012; 69 (1): 207-213.
 
[31]  Pogach LM, Lee Y, Giglio W, Naumoff M, Huang HF. Zinc acetate pretreatment ameliorates cisplatin-induced Sertoli cell dysfunction in Sprague-Dawley rats.Cancer Chemother Pharmacol 1989; 24 (3): 177-180.
 
[32]  Ateşşahin A, Sahna E, Türk G, Ceribaşi AO, Yilmaz S, Yüce A, Bulmuş O. Chemoprotective effect of melatonin against cisplatin-induced testicular toxicity in rats. J Pineal Res 2006; 41 (1): 21-27.
 
[33]  Silici S, Ekmekcioglu O, Eraslan G, Demirtas A. Antioxidative effect of royal jelly in cisplatin-induced testes damage. Urology 2009; 74 (3): 545-551.
 
[34]  Rezvanfar MA, Rezvanfar MA, Shahverdi AR, Ahmadi A, Baeeri M, Mohammadirad A, Abdollahi M. Protection of cisplatin-induced spermatotoxicity, DNA damage and chromatin abnormality by selenium nano-particles. Toxicol Appl Pharmacol 2013: 266 (3): 356-365.
 
[35]  Waseem M, Parvez S. Mitochondrial dysfunction mediated cisplatin induced toxicity: modulatory role of curcumin. Food Chem Toxicol 2013; 53: 334-342.
 
[36]  Keshtmand Z, Oryan S, Ghanbari A, Khazaei M. Protective Effect of Tribulus terrestris Hydroalcoholic Extract against Cisplatin-Induced Cytotoxicity on Sperm Parameters in Male Mice. Int J Morphol 2014; 32 (2): 551-557
 
[37]  Richie JP, Jr, Skowronski L, Abraham P, Leutzinger Y. Blood glutathione concentrations in a large-scale human study. Clin Chem 1996; 42 (1): 64-70.
 
[38]  Kandemir, FM, Benzer F, Yildirim NC, Ozdemir N. Compensatory effects of curcumin on cisplatin-induced toxicity in rabbit testis. J Med Plants Res 2011; 5 (3) 456-461.
 
[39]  Serafini M, Del Rio D. Understanding the association between dietary antioxidants, redox status and disease: is the total antioxidant capacity the right tool? Redox Rep 2004; 9 (3): 145-152.
 
[40]  Anand H, Misro M, Sharma SB, Prakash S. Protective effects of Eugenia jambolana extract versus Nacetyl cysteine against cisplatin-induced damage in rat testis. Andrologia 2014; 10 (2): 291-297.
 
[41]  Watcho P, Kamtchouing P, Sokeng SD, Moundipa PF, Tantchou J, Essame JL. Androgenic effect of Mondia whitei roots in male rats. Asian J Androl 2004; 6 (3): 269-272.
 
[42]  Kasturi M, Manivannan B, Ahmed NR, Shaikh PD, Pattan KM. Changes in epididymal structure and function of albino rat treatedwith Azardirachta indica leaves. Indian J Expt Biol 1995; 33 (10): 725-729.
 
[43]  Yakubu MT, Akanji MA, Oladiji AT. Effects of oral administration of aqueous extract of Fadogia agrestis (Schweinf. Ex Hiern) stem on some testicular function indices of male rats. J Ethnopharmacol 2008; 115 (2): 288-292.
 
[44]  Kamel KM, Abd El-Raouf OM, Metwally SA, Abd El-Latif HA, El-Sayed ME. Hesperidin and rutin, antioxidant citrus flavonoids, attenuate Cisplatin-induced nephrotoxicity in rats. J Biochem Mol Toxicol 2014; 28 (7): 312-319.