Journal of Cancer Research and Treatment
ISSN (Print): 2374-1996 ISSN (Online): 2374-2003 Website: http://www.sciepub.com/journal/jcrt Editor-in-chief: Jean Rommelaere
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Journal of Cancer Research and Treatment. 2014, 2(3), 55-63
DOI: 10.12691/jcrt-2-3-3
Open AccessArticle

The Effect of L-carnitine on Amethopterin-induced Toxicity in Rat Large Intestine

Ehab Tousson1, , Mona Hegazy1, Ezar Hafez1 and Alaa Abdullah Ahmed1

1Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt

Pub. Date: November 02, 2014

Cite this paper:
Ehab Tousson, Mona Hegazy, Ezar Hafez and Alaa Abdullah Ahmed. The Effect of L-carnitine on Amethopterin-induced Toxicity in Rat Large Intestine. Journal of Cancer Research and Treatment. 2014; 2(3):55-63. doi: 10.12691/jcrt-2-3-3

Abstract

Background: Amethopterin is a folic acid antagonist which used as a chemotherapeutic agent and its anti-oxidant activity is used to treat many cancer types. This study aimed to determine the possible protective effects of L-carnitine against Amethopterin induced large intestine toxicity. Methodology: A total 60 male albino rats were equally divided into six groups; the first and second groups were the control and L-carnitine groups respectively while the 3rd group was Amethopterin rat group; the 4th and 5th groups were co- and post- treated Amethopterin rat with L-carnitine respectively and the 6th group was self treated Amethopterin rat group. Results: Glutathione, catalase and total protein levels in Amethopterin and self-treated groups showed a significant decrease when compared with control group, while MDA levels in Amethopterin and self-treated groups showed a significant increase when compared with control group. Many of abnormalities as colonic epithelial cell damage in the form of epithelial separation, cellular loss, congestion of blood vessels, crypt hyperplasia and focal depletion of goblet cells were detected in large intestine tissues in Amethopterin rat group (Amethopterin and self-treated groups). A significant increase of the apoptotic protein p53 and a significant decrease in the antiapoptotic Bc1-2 proteins after Amethopterin injection when compared with control group was observed. Conclusions: Treatment (Co- and post-) with L-carnitine were improved the biochemical, histopathological and immunohistochemical alterations in large intestine that treated with Amethopterin.

Keywords:
Amethopterin large intestine L-carnitine oxidative stress apoptotic markers P53 Bcl2

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