Journal of Biomedical Engineering and Technology
ISSN (Print): 2373-129X ISSN (Online): 2373-1303 Website: http://www.sciepub.com/journal/jbet Editor-in-chief: Ahmed Al-Jumaily
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Journal of Biomedical Engineering and Technology. 2019, 7(1), 5-13
DOI: 10.12691/jbet-7-1-2
Open AccessArticle

Functionals Aspects and Simultaneous Detection of Dopamine, Ascorbic Acid and Uric Acid Using Chitosan-Catechol Graphene and Carbon Nanotube Modified Electrodes

Tjerignimin Adissa Silue1, , Valerie Lewitus2, Abul Hussam3, Hadar Ben-Yoav4 and Nathalia Peixoto1

1Bioengineering, George Mason University, Fairfax, USA

2Neuroscience, George Mason University, Fairfax, USA

3Chemistry and Biochemistry, George Mason University, Fairfax, USA

4Biomedical Engineering, Ben-Gurion University of the Negev, Sheva, Israel

Pub. Date: November 28, 2019

Cite this paper:
Tjerignimin Adissa Silue, Valerie Lewitus, Abul Hussam, Hadar Ben-Yoav and Nathalia Peixoto. Functionals Aspects and Simultaneous Detection of Dopamine, Ascorbic Acid and Uric Acid Using Chitosan-Catechol Graphene and Carbon Nanotube Modified Electrodes. Journal of Biomedical Engineering and Technology. 2019; 7(1):5-13. doi: 10.12691/jbet-7-1-2

Abstract

Dopamine functions as a neurotransmitter in the brain. The dysfunction of the dopaminergic system is the leading cause of numerous diseases such as Parkinson’s disease. Hence, it is important to find selective and sensitive detection methods for the early diagnosis of diseases related to the abnormal levels of dopamine. In this study, we show a new electrochemical sensing platform based on carbon nanotube (SWCNT) and a sheet of graphene (GRA). The novelty of our sensor is the coating of the substrates with chitosan-catechol (CC) by electrodeposition, enhancing the dopamine response by 70%. The dose-response for each set of electrodes (bare CNT, bare GRA, and coated CNT as well as coated GRA) was measured. Finally, the electrodes were tested in cerebrospinal fluid (artificial and human), for the detection of millimolar to nanomolar levels of dopamine. The electrodes exhibited high sensitivity (2.03mA mol·L-1, 1.45 mA mol·L-1, 0.0298 mA mol·L-1, and 0.0559 mA mol·L-1 for the modified CNT, bare CNT, modified GRA and bare GRA, respectively, for the oxidation of DA. The oxidation peak current was proportional to the concentration of DA in the range from 50×10-6 to 50x10-9 M (n= 6, r2 =0.98). The dopamine recovery in human CSF were, 49-78% and 65-65% with coated graphene and CNT electrodes, respectively. Our results indicate that the CC modified CNT electrodes achieved the best recovery, sensitivity, limit of detection, and selectivity compared to the uncoated CNT as well as the coated and uncoated graphene electrodes.

Keywords:
dopamine uric acid ascorbic acid cyclic voltammetry limit of detection sensitivity cerebrospinal fluid

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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