Journal of Applied & Environmental Microbiology
ISSN (Print): 2373-6747 ISSN (Online): 2373-6712 Website: http://www.sciepub.com/journal/jaem Editor-in-chief: Sankar Narayan Sinha
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Journal of Applied & Environmental Microbiology. 2018, 6(1), 18-24
DOI: 10.12691/jaem-6-1-3
Open AccessArticle

Isolation and Molecular Characterization of Soil Bacteria Capable of Degrading Chlorpyrifos and Diuron Pesticides

Mirenga E.O1, Korir J.C1, , Kimosop S.J2, Orata F2 and Getenga Z.M3

1Department of Biological Sciences, Masinde Muliro University of Science and Technology, Kakamega, Kenya

2Department of Pure & Applied Chemistry, Masinde Muliro University of Science and Technology, Kakamega, Kenya

3Department of Physical Sciences, Chuka University, Chuka Kenya

Pub. Date: February 07, 2018

Cite this paper:
Mirenga E.O, Korir J.C, Kimosop S.J, Orata F and Getenga Z.M. Isolation and Molecular Characterization of Soil Bacteria Capable of Degrading Chlorpyrifos and Diuron Pesticides. Journal of Applied & Environmental Microbiology. 2018; 6(1):18-24. doi: 10.12691/jaem-6-1-3

Abstract

The pesticides Chlorpyrifos and Diuron have been extensively used in sugarcane farming for several years in spite of their known harmful effects. The aim of this study was to isolate Chlorpyrifos and Diuron- degrading bacteria from exposed agricultural soil in the Nzoia River Drainage Basin and characterize the isolated bacteria by analyzing the 16S rRNA nucleotide sequence. The isolates could be potential candidates for use in bioremediation protocols. One soil isolate was found capable of degrading Chlorpyrifos and another was found capable of degrading Diuron. 16S rRNA gene sequences of the two isolates were deposited in GenBank and assigned the Accession Numbers MG517447 and MG517448 respectively. Sequence analysis of the two isolates using BLASTN and phylogenetic analysis revealed that the isolate capable of utilizing Chlorpyrifos as the sole carbon source was Kosakonia oryzae strain Ola 51, while the isolate capable of utilizing Diuron as the sole carbon source was Pseudomonas aeruginosa strain M-1. Kosakonia oryzae strain Ola 51 and Pseudomonas aeruginosa strain M-1 are thus potential candidates for use in bioremediation protocols for soils contaminated with Chlorpyrifos and Diuron, respectively.

Keywords:
Chlorpyrifos Diuron Kosakonia oryzae Pseudomonas aeruginosa

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