Etiologic Agents and Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Urines of Antiretroviral Therapy Users

Jean-Marie Liesse Iyamba^1, , Cyprien Mbundu Lukukula¹, Daniel Tassa Okombe¹, José Mulwahali Wambale¹, Joseph Welo Unya¹, Ickel Kakongo Kabangwa², Christian Hemedi Asani³ and Takaisi Kikuni NB¹

¹Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo

²Unité d’Enseignement et de Recherche de Microbiologie, Section Techniques de Laboratoire, Institut Supérieur des Techniques Médicales, Kinshasa, Democratic Republic of Congo

³Laboratoire de Bactériologie de l’Hôpital de l’Amitié Sino-Congolaise, Kinshasa, Democratic Republic of Congo

Cite this paper:
Jean-Marie Liesse Iyamba, Cyprien Mbundu Lukukula, Daniel Tassa Okombe, José Mulwahali Wambale, Joseph Welo Unya, Ickel Kakongo Kabangwa, Christian Hemedi Asani and Takaisi Kikuni NB. Etiologic Agents and Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Urines of Antiretroviral Therapy Users. Journal of Applied & Environmental Microbiology. 2016; 4(4):85-87. doi: 10.12691/jaem-4-4-3

Abstract

The aim of this study was to determine the etiologic agents and the antibiotic susceptibility pattern of Staphylococcus aureus isolated among antiretroviral therapy users with asymptomatic urinary tract infections. Urine samples were collected for the diagnostic purpose. Uropathogen bacteria were identified using microbiological conventional methods, and the antibiotic susceptibility test was carried out using disk diffusion method. The predominant microorganisms isolated were Escherichia coli (49.6 %) followed by coagulase negative staphylococci (18.4 %) and Staphylococcus aureus (14.1%). All Staphylococcus aureus identified were resistant to methicillin and multidrug resistant. The results of the present study suggest the use antibiotic susceptibility test before treatment of urinary tract infection in human immunodeficiency virus infected patients.

Keywords:
human immunodeficiency virus etiologic agents Staphylococcus aureus antibiotics Democratic Republic of Congo

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References:

[1]	Heyns C. F, Fisher M. (2005). The urological management of the patient with acquired immunodeficiency syndrome. BJU Int, 95: 709-716.
[2]	Jombo G. T, Egaz D. Z, Ayeni J. A. (2005). Bacteriology of urinary tract infection among patients with acquired immunodeficiency syndrome in Jos, Nigeria. Niger J Med, 14: 422-424.
[3]	Kaplan M. S, Wechsler M, Benson M. C. (1987). Urologic manifestations of AIDS. Urology, 30: 441-443.
[4]	Miles B. J, Melser M, Farah R, Markowitz N, Fisher E. (1989).The urological manifestations of the acquired immunodeficiency syndrome. J Urol, 142: 771-773.
[5]	Olowe O. A, Ojo-Johnson B. B, Makanjuola O. B, Olowe R. A, Mabayoje V. O. (2015). Detection of bacteriuria among human immunodeficiency virus seropositive individuals in Osogbo, south-western Nigeria. Eur J Microbial Immunol, 5: 126-130.
[6]	Iweriebor B. C, Obi C. L, Akinyemi O, Ramalivhana N. J, Hattori T, Okoh A. I.(2012). Uropathogens isolated from HIV-infected patients from Limpopo Province, South Africa. Afr J Biotechn, 11: 10598-10604.
[7]	Debalke S, Cheneke W, Tassew H, Awol M. (2014). Urinary tract infection among antiretroviral therapy users and nonusers in Jimma University Specialized Hospital, Jimma, Ethiopia. Int J Microbiol.
[8]	Iyamba J. M, Wambale J. M, Takaisi-Kikuni N. B. (2014). Antimicrobial susceptibility patterns of enterobacteriaceae isolated from HIV-infected patients in Kinshasa. Pan Afr Med J, 17:179.
[9]	Clinical and Laboratory Standards Institute (CLSI). (2011). Performance standards for antimicrobial susceptibility testing. Approved standard. CLSI document M100-S21. Wayne, PA: CLSI.
[10]	Inyang-Etoh P. C, Udofia G. C, Alaribe A.A.A, Udonwa N. E. (2009). Asymptomatic bacteriuria in patients on antiretroviral drug therapy in Calabar. J Med Sci, 9: 270-275.
[11]	Omoregie R and Eghafona N. O. (2009). Urinary tract infection among asymptomatic HIV patients in Benin City, Nigeria. Br J Biomed Sci, 66:190-193.
[12]	Arshed S, Luo H, Middleton J, Yousif A. (2015). An Unusual Case: Salmonella UTI and Orchitis in HIV Patient. Case Rep Med.