Journal of Applied & Environmental Microbiology
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Journal of Applied & Environmental Microbiology. 2016, 4(4), 75-84
DOI: 10.12691/jaem-4-4-2
Open AccessArticle

Bioremediation of Crude Oil-polluted Soil Using Broiler-Chicken Droppings

Ugochukwu C. Okafor1, , Michael U. Orji1, Kingsley C.Agu1, Nsikak S. Awah1, Benjamin C. Okeke1, Onyedika I. Okafor1 and Nnenna C.N. Okoro1

1Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

Pub. Date: July 11, 2016

Cite this paper:
Ugochukwu C. Okafor, Michael U. Orji, Kingsley C.Agu, Nsikak S. Awah, Benjamin C. Okeke, Onyedika I. Okafor and Nnenna C.N. Okoro. Bioremediation of Crude Oil-polluted Soil Using Broiler-Chicken Droppings. Journal of Applied & Environmental Microbiology. 2016; 4(4):75-84. doi: 10.12691/jaem-4-4-2

Abstract

The impact of poultry droppings in the bioremediation of crude oil-polluted soil was evaluated. Different concentrations of the poultry droppings (10%, 30%, and 50%) were also studied. The physicochemical and microbiological properties of the soil were monitored for a period of 6 months. The poultry droppings had total heterotrophic bacterial and fungal counts of 4.2 × 104cfu/g and 1.8 × 104 cfu/g respectively. The total hydrocarbon utilizers increased progressively from month 2 to month 3, after which a decline from month 4 down occuured. The total heterotrophic microbial counts also increased from month 2 to month 4 followed by a decline from month 5 down. The control showed slight increase in microbial growth. The microbial growth rate increased as the concentration of the poultry droppings increased. Statistical analyses showed a significant difference at (P<0.05), level for the amended options and control. The total hydrocarbon content of the oil-polluted soil decreased from 6609.83 to 2951.37ml/g. Bacillus spp Pseudomonas spp Flavobacterium spp Fusarium spp, Aspergillus spp were isolated. Alkaline pH was observed in the poultry droppings as well as in the amended soils at 50% and lowest at the control. Ecotoxicity assay, measured in terms of germination index was used to evaluate the extent of contaminant removal.. Using seeds of Viciafaba, germination index of 95 % was observed in the 50 % amended option only. The study therefore showed that poultry droppings can serve as a good remediation material in the reclamation of a crude oil-polluted lithosphere.

Keywords:
bioremediation crude oil polluted soil chicken droppings amendment

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