Journal of Applied & Environmental Microbiology
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Journal of Applied & Environmental Microbiology. 2016, 4(4), 75-84
DOI: 10.12691/jaem-4-4-2
Open AccessArticle

Bioremediation of Crude Oil-polluted Soil Using Broiler-Chicken Droppings

Ugochukwu C. Okafor1, , Michael U. Orji1, Kingsley C.Agu1, Nsikak S. Awah1, Benjamin C. Okeke1, Onyedika I. Okafor1 and Nnenna C.N. Okoro1

1Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

Pub. Date: July 11, 2016

Cite this paper:
Ugochukwu C. Okafor, Michael U. Orji, Kingsley C.Agu, Nsikak S. Awah, Benjamin C. Okeke, Onyedika I. Okafor and Nnenna C.N. Okoro. Bioremediation of Crude Oil-polluted Soil Using Broiler-Chicken Droppings. Journal of Applied & Environmental Microbiology. 2016; 4(4):75-84. doi: 10.12691/jaem-4-4-2


The impact of poultry droppings in the bioremediation of crude oil-polluted soil was evaluated. Different concentrations of the poultry droppings (10%, 30%, and 50%) were also studied. The physicochemical and microbiological properties of the soil were monitored for a period of 6 months. The poultry droppings had total heterotrophic bacterial and fungal counts of 4.2 × 104cfu/g and 1.8 × 104 cfu/g respectively. The total hydrocarbon utilizers increased progressively from month 2 to month 3, after which a decline from month 4 down occuured. The total heterotrophic microbial counts also increased from month 2 to month 4 followed by a decline from month 5 down. The control showed slight increase in microbial growth. The microbial growth rate increased as the concentration of the poultry droppings increased. Statistical analyses showed a significant difference at (P<0.05), level for the amended options and control. The total hydrocarbon content of the oil-polluted soil decreased from 6609.83 to 2951.37ml/g. Bacillus spp Pseudomonas spp Flavobacterium spp Fusarium spp, Aspergillus spp were isolated. Alkaline pH was observed in the poultry droppings as well as in the amended soils at 50% and lowest at the control. Ecotoxicity assay, measured in terms of germination index was used to evaluate the extent of contaminant removal.. Using seeds of Viciafaba, germination index of 95 % was observed in the 50 % amended option only. The study therefore showed that poultry droppings can serve as a good remediation material in the reclamation of a crude oil-polluted lithosphere.

bioremediation crude oil polluted soil chicken droppings amendment

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[1]  Jobson, A. M., Mclaughlin, M., Cook, F. D.,Westlake, D.W.S. 1974. Effects of amendment on microbial utilization of oil applied to soil, Appl. Microbiol. 27, 166-171.
[2]  Amadi, A..J. 1992. A double control approach to assessing the effect of remediation of preplan ting oil pollution on maize growth.Delta Agric. J. Nigeria.1, 1-6.
[3]  Ekpo, M. A., and Udofia, U. S. 2008. Rate of Biodegradation of Crude Oil by Microorganisms isolated from Oil Sludge Environment. Afr. J. Biotechnol. 7, 4495-4499.
[4]  Reisinger, H. J. 1995.. Hydrocarbon bioremediation an overview. In: Hinchee. R. E., Kittel, J. A. and Reisinger, H. J.(Eds.). Applied Bioremediation of Petroleum Hydrocarbons. Battelle Press, Columbus. 7.
[5]  Dutta, T. K. and Harayama, S. 2001. Analysis of long side chain alkyl aromatics in crude oil for evaluation of their fate in the environment.Environ.Sci. Technol. 35, 102-107.
[6]  Gerhart, P. Murray., R. R. E., Kneg, N. R., Phillips, C. B. 1981.Manual of Methods of General Bacteriology.American Society for Microbiology, Washington D.C. W. A.
[7]  Marshal. T. R. 1988. Biodegradation of Petroleum wastes in soil. The Microbial Ecosystem and the Optimization of a Treatment Process.DoctoralThesis.University of Southern California, California, USA.
[8]  Nwankwegu, A.S., Onwosi, C.O., Orji, M.U., Anaukwu, C.G., Okafor, U.C., Azi, F., Marins, P.E. 2016. Reclamation of DPK hydrocarbon polluted agricultural soil using a selected bulking agent. J.Environ. Manag. 172, 136-42.
[9]  Romantschuk, M., Sarand, I., Petanen, T., Peltola, R., Jonsson-vihanne, M.,Koivula, T. 2000. Means to improve the effect of in situ bioremediation of contaminated soil: an overview of novel approaches. Environ. Pollut. 107, 179-185.
[10]  Morgan, W. W., and Watkinson, G. R. 1989.Hydrocarbon degradation in soils and methods for soil biotreatment Criteria Revolution Biotechnology. 8, 305 -333.
[11]  Walker. J. D. 1976. Enumeration of Petroleum -Degrading Microorganisms. Appl. Environ. Microbiol.31, 198-207.
[12]  Andreolli, M., Lampis, S., Brignoli, P., Vallini, G. 2015. Bioaugmentation and biostimulation as strategies for the bioremediation of a burned woodland soil contaminated by toxic hydrocarbons: A comparative study. J. Environ. Manage. 153, 121-131.
[13]  McGill, W. B., and Nyborg, M. F. 1975. Reclamation of Wet Forest Soils Subjected to Oil Spills. Alberta institute of Pedology.Cannada.Pub.No.G-75-1.
[14]  Atlas, R. M. 1981. Biodegradation of Petroleum Hydrocarbon: An Environmental Perspective Microbiological Review 45,180-209.
[15]  Baker, J. M. 1970. The Effects of Oil on Plants. Environmental Pollution 1, 27-44.
[16]  Plice, M. J., 1948. Some effect of crude petroleum on soil fertility. Soil Science Society Proceedings.13, 413-416.
[17]  Toogood. J, A. 1974.Effects of Light Oil Spills on Crop Growth. In: Proceedings of the Workshop on Reclamation of Disturbed lands in Aberta. Alberta, Canada.
[18]  Horowit, J. U., and Atlas, R. M. 1978.Microbial seeding to enhance petroleum hydrocarbon biodegradation in aquatic ecosystems. Biodeterioration. Proceedings Of the Fourth International Symposium. 49, 79-92.
[19]  Yeung, P. Y., Johnson, R. L., Xu, J. G. 1997. Biodegradation of Petroleum hydrocarbons in soil as affected by healing and forced aeration, J. Environ.Qual. 26, 1511-1516.
[20]  Isirimaru, N. O., Zoufa, K.,Loganathan, P. 1989. Effect of Crude oil on maize performance and soil chemical properties in humid forest zone of Nigeria. Discovery and Innovation. 1, 95-98.
[21]  Adeniyi, E. O., Oluwole, B. A., Amyaye, G. K. 1983. Environmental and social economic impacts of oil spillage in the petroleum producing service area of Nigeria. Environmental Impact Statement and Assessment.8, 233-237.
[22]  Adams, P.Z., Jackson, P. P. 1996.Bioremediation of oil spill.Theory and Practice.In Nigeria National Petroleum Corporation Publication.Department of Petroleum Resources. 3-10.
[23]  Odgen, R., and Adams, D. A., 1989. Recombinant DNA Technology: Applications In: Carolina Tops, Vol. 52, Carolina Biological Supply company, Burlington, North Carolina. 18-19.
[24]  Keeney, D. R., and Nelson, D. W. 1994. Determination of numeral (inorganic) Nitrogen. In: Miller, A. L, and Keeney, D. R. (Ed.). Methods of Soil Analysis. Animal Science Publishers. Madison. 643.
[25]  Odu, C. T., Ogunbi, A. A., Fagade, O. F. 1989. Microbiology of Soil Contaminated with Petroleum Hydrocarbon in Natural Rehabilitation and Reclamation Petroleum Technology Publication, Warri Nigerian. 11-48.
[26]  Office of Technology Assessment (OTA) 1990. Bioremediation of marine oil spill. Workshop. Government Printing Press. Washington, D. C. 4, 1-30.
[27]  Ijah, U. J., and Antai, S. P. 2003. The Potential use of chicken droppings microorganisms for oil spill remediation. The Environmentalist.23, 89-95.
[28]  Tinely, P. Y., Yu, K., Thomas, J. W. 1967. Animal wastes as Microbial Labitato, Oxford University Press, London.
[29]  Herman, D. C., Artiola, J, F, Miller, R. M. 1995. Removal of Cadmium. Lead and Zinc from, soil by a Rhamnophical Biosurfactant. Environ. Sci.Technol.29, 2280-2285.
[30]  Atlas, R. M. 1995. Petroleum biodegradation and oil spill bioremediation. Microbiological Review.45, 180-209.
[31]  Atlas, R. M., Bartha, R. 1973.Fate and effects of polluting petroleum on the marine environment.Microbiology Review 49, 49-85.
[32]  Okafor U.C, Orji M.U. 2016.Effects of Chicken droppings amendment on bioremediation of crude oil polluted soil. European Journal of Experimental Biology 6, 62-68.
[33]  Black, C. A. 1965.Methods of Soil Analysis. Madison: American Society of Agronomy, Madison, Wisconsin. 5 - 25.
[34]  Bray. R., H and Kurtz, L. T. 1945.Determination of Total Organic and Available Forms of Phosphorus in Soils.Soil Sci. 59, 39-54.
[35]  Murphy, K. M., and Riley, J. P. 1962. Trace metal speciation Analytical Method and problems. Analytical Chemical, Act 227, 31-36.
[36]  Thomas, G. W. 1982. Exchangeable cations, Pgs 159-165 In: Methods of soil analysis. Part II (page A. L Miller. R. H., and Keeney. D. R., eds,). 2nd edition.America Society of Agronomy and Soil Science of America. Madison. Wisconsin, USA.
[37]  Amadi, A. J., Abbey, S. D., Anthony, N. U. 1993. Chronic Effects of Oil Spillon Soil Properties Micro-floras of a Rain Forest Ecosystem in Nigeria.Water, Air and Soil Pollut.86: 1
[38]  Atlas, R. M., and Bartha, R. 1992.Hydrocarbon biodegradation and oil-spill bioremediation. In: Marshall, K. C. (ed.). Advances in Microbial Ecology.Plenum Press, New York. 12, 287-338.
[39]  Collins C. H., and Lyne, P. M. 1976.Microbiological Methos.4lhed, London: Butterworth.
[40]  Okpokwasili, G. C., and Amanchukwu, S. C. 1988. Petroleum Hydrocarbon Degradation by Candida species. Environ. Int. 4, 234-247.
[41]  Okpokwasili, G.C., and Ibe, S. 1989. Incidence of Hydrocarbon-Degrading Bacteria in Crude Oil, Kerosene and Petrol. Bulletin of Biotechnology Society of Nigeria. Paper Presented at 3rd National Conference on Biotechnology, Enugu, Nigeria.
[42]  Chikere, C.B., Chikere B.O and Okpokwasili, G.C. (2012). Bioreactor-based bioremediation of hydrocarbon-polluted Niger Delta marine sediment, Nigeria J. Biotechnology 2: 53-66.
[43]  Thijsee, G. J., and Vander Linden, A. C. 1961.Iso-alkane Oxidation by a Pseudomonas.Antonie van Leuwenhoek J. Microbiol. 27, 171-179.
[44]  Odu, C. T. I. 1981. Degradation and Weathering of crude oil under Tropical Condition.Proceeding of the International Seminar on the Petroleum Industry and the Nigerian Environment, Warri, Nigeria. 143-153.
[45]  Yakubu, M. J. 2007. Biodegradation of Lagoma Crude Oil using Pig Dung. J. Microbiol.6, 2821-2828.
[46]  Laukova, A. K. 2000. In Vitro Treatment of Hydrocarbons by members of the genus Candida.J. Bacteriol.93, 1847-1852.
[47]  Mansour, M. A., Bottegfroy, D., Lindcr, A. 1999. Inhibition of Bacillus licheniformisspore growth in milk by nisin, monolaurin, and pH combinations.J. Appl. Microbiol.86, 311-324.
[48]  Bhatta-chyrn, C. L., Fontenol, J. P. 1966. Value of Broiler litter as Feed for Ruminants.ProceedingsOf National Symposium On Animal Science. Waste Manag.366, 105.
[49]  Pritchard, H.P. 1991.EPA's Alaska oil spill Bioremediation Project. Sc.Technol. 25, 372-379.
[50]  Flegal, C. J., and Zindal, R. C. 1970.The Utilization of Poultry Waste as Stuff for Growing Chick.Research report No. 117, Michigan State Agricultural Experiment Station, East Lansing, Michigan. 21.
[51]  Watkinson, R. J. and Morgan, P. 1990.. Physiology of Aliphatic Hydrocarbon- Degrading Microorganisms. Biodegradation. 1, 79-92.
[52]  Dotson, G. K., Dean, R. B., Cooke, W. B., Kenner, B. A. 1972. Land Treatment Spreading, a Conserving and Non-pollution Method of Disposal of Oily Waste. In: Reprocessing and Disposal of Waste Petroleum Oils: L. Y Press Noyes Data Crops. 259-267 Educational Books, New York, 436-447.