Journal of Applied & Environmental Microbiology
ISSN (Print): 2373-6747 ISSN (Online): 2373-6712 Website: Editor-in-chief: Sankar Narayan Sinha
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Journal of Applied & Environmental Microbiology. 2016, 4(2), 39-45
DOI: 10.12691/jaem-4-2-3
Open AccessArticle

Effect of Sourdough on Shelf Life, Freshness and Sensory Characteristics of Egyptian Balady Bread

Sobhy. M. Mohsen1, Mohamed H. Aly1, Afaf A. Attia2 and Dalia B. Osman2,

1Faculty of Agriculture, Food industries department, Cairo University, Giza, Egypt

2Bread and Pasta Department, Food Technology Research Institute, Agricultural Research Center, Giza, Egypt

Pub. Date: May 20, 2016

Cite this paper:
Sobhy. M. Mohsen, Mohamed H. Aly, Afaf A. Attia and Dalia B. Osman. Effect of Sourdough on Shelf Life, Freshness and Sensory Characteristics of Egyptian Balady Bread. Journal of Applied & Environmental Microbiology. 2016; 4(2):39-45. doi: 10.12691/jaem-4-2-3


The present study was aimed to improve the quality characteristics of Egyptian balady bread by using sourdough containing (2% Saccharomyces cerevisiae+ 1, 2 or 3% Lactobacillus plantarum). Microbial contents i.e. lactic acid bacteria, total bacterial count and yeasts, pH, organic acids and antimicrobial activity were evaluated during sourdough fermentation. Results showed an increase in organic acids, antimicrobial activity and reduction in pH during the preparation of different sourdough samples. These metabolites were increased by increasing lactic acid bacteria ratio in sourdough (3%> 2%> 1% Lb. plantarum). A significant reduction in total bacterial count and a significant increase in LAB and yeast count during fermentation period was recorded. Bread characteristics showed an extension of shelf life for 8 days for bread samples containing sourdough (2 or 3% Lb. plantarum) comparing to 3 days for control bread. Addition of 20% sourdough containing 2 or 3% Lb. plantarum to wheat flour dough also retarded staling rate by 19.98% and 19.30% after 3 days comparing to control sample was (42.84%). Improvements in sensory characteristics and acceptability of balady bread were also recorded. Accordingly, this could reduce the bread losses and consequently reduce the amount of wheat flour used.

sourdough lactic acid bacteria antimicrobial activity organic acids shelf life staling and sensory evaluation

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[1]  Katina, K. (2005). Sourdough: A tool for the improved flavor, texture and shelf life of wheat bread. Vtt Technical Research Center of Finland, Vtt Publications.
[2]  Arendt, E.K.; L.A.M. Ryan and F.D. Bello (2007). Impact of sourdough on the texture of bread. Food microbial., 24: 165-174.
[3]  De Vuyst, L. and P. Neysens (2005). Biodiversity and identification of sourdough lactic acid bacteria. Food Microbiol., 24(2): 120-127.
[4]  Gobbetti, M., M. Angelis, A. Corsetti and R. Di Cagno (2005). Biochemistry and physiology of sourdough lactic acid bacteria. Trends Food Sci. Technol., 16 (1-3): 57-69.
[5]  Paramithiotis, S.; S. Gioulatos; E. Tasakalidou and G. Kalantzopoulos (2006). Interactions between Saccharomyces cervisiae and Lactic acid bacteria in sourdough. Process Biochem., 41: 2429-2433.
[6]  Corsetti, A. and L. Sattani (2007). Lactobacilli in sourdough fermentation. Food Res. Int., 40: 539-558.
[7]  Corsetti, A., L. Settanni, S. Valmorri, M. Mastrangelo and G. Suzzi (2007). Identification of subdominant sourdough lactic acid bacteria and their evolution during laboratory scale fermentation. Food Microbiol., 24(6): 592-600.
[8]  Stolze, P. and G. Böcker (1996). Technology, properties and applications of sourdough products. Advances in Food Sci., 18: 234-236.
[9]  Hammes, W.P. and M.G. Gänzle (1998). Sourdough breads and related products. In: Microbiology of fermented foods. PP. 199-216. Edited by B.J.B. Wood, ed. Blackie Academic and Professional: London.
[10]  Corsetti, A.; M. Gobbetti; J. Rossi and P. Damiani (1998). Antimould activity of sourdough lactic acid bacteria: identification of mixture of organic acids produced by lactobacillus sanfrancisco CB1. Appl. Microbiol. Biotechnol. 50: 253-256.
[11]  Corsetti, A., M. Gobetti, B. De Marco, F. Balestrieri, F. Paoletti, L. Russi and J. Russi (2000). Combined effect of sourdough lactic acid bacteria and additives on bread firmness and staling. J. Agric. Food Chem., 48: 3044-3051.
[12]  Katina, K.; M. Sauri; H.L. Alakomi and T. Mattila-Sandholm (2002). Potential of lactic acid bacteria to inhibit rope spoilage in wheat sourdough bread. LWT, 35: 38-45.
[13]  Thiele, C.; M.G. Gänzle and R.F. Vogel (2002). Contribution of sourdough lactobacilli, yeast, and cereal enzymes to the generation of amino acids in dough relevant for bread flavor. Cereal Chem., 79: 45-51.
[14]  Clarke, C.; T. Schober; P. Dockery; K. Ơsullican and E. Arendt (2004). Wheat sourdough fermentation: effects of time and acidification on fundamental rheological properties. Cereal Chem., 81 (3): 409-417.
[15]  Katina, K.; M. Samenkallio-Marttilla; R. Partanen; P. Forssell and K. Autio (2006). Effects of sour dough and enzymes on staling of high fibre wheat bread LWT – Food Sci. and Technol., 39 (5): 479-491.
[16]  Ur- Rehman, S.; H. Nawaz; S. Hussain; M. M. Ahmad; M. A. Murtaza and M. S. Ahmad (2006). Effects of sourdough bacteria on the quality and shelf life of bread. Pakistan J. Nutr. 6(6): 562-565.
[17]  Dalbello, F., C.I. Clarke, L.A.M. Ryan, H. Ulmer, T.J. Shober, K. Ström, J. Sjögren, D. Van Sinderen, J. Schnürer and E.K. Arendt (2007). Improvement of the quality and shelf life of wheat bread by fermentation with the antifungal strain Lactobacillus Plantarum FST 1.7. J. Cereal Sci., 45(3): 309-318.
[18]  De Vuyst, L. and Vancanneyt, M. (2007). Biodiversity and identification of sourdough lactic acid bacteria. Food Microbiol. 24: 120-127.
[19]  Mentes, Ö.; R. Ercan and M. Akcelik (2007). Inhibitor activities of two lactobacillus strains, isolated from sourdough, against rope- forming Bacillus strains. Food Microbiol., 18: 359-363. (c.f. Ahmed, M.A.A. (2009). M.R.G. M. Sc., Thesis, Fac. of Agric. Ain Shams Univ).
[20]  Sadeghi, A. F. Shahidi, S.A. Mortazavi, A. Koocheki and M. Mohebbi (2007). Evaluation of sourdough effect on Iranian Barbari bread staling. World Applied Sci. J., 2(5): 490-498.
[21]  Sadeghi, A. (2008). The secrets of sourdough: a review of microflora potential of sourdough in bread shelf life. Biotechnol., 7(3): 413-417.
[22]  Ogunbanwo, S.T.; A.I. Sanni and A.A. Onilude (2003). Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OGI. African J. Biotechnol., 2 (8): 219-227.
[23]  Messens, W. and L. De Vuyst (2003). Inhibitory substances produced by Lactobacilli isolated from sourdoughs: A review. Int. J. Food Microbiol., 72(1-2): 31-43.
[24]  Clarke and Arendt, (2005). A Review of the Application of Sourdough Technology to Wheat Breads. Advances in Food and Nutrition Research 49: 137-161.
[25]  Yousif, M.R.G. (2011). Studies on production of some functional bakery products. Ph.D. Thesis. Fac. of Agric. Ain Shams Univ.
[26]  Lavermicocca, P.; F. Valerio; A. Evidente; S. Lazzaroni; A. Corsetti and M. Gobbetti (2000). Purification and characterization of novel antifungal compounds from the sourdough Lactobacillus plantarum strain 21B. Appl. and Environ. Microbiol., 66(4): 4084-4090.
[27]  Cizeikiene, D.; G. Juodeikiene; A. Paskevicius and E. Bartkiene (2013). Antimicrobial activity of lactic acid bacteria against pathogenic and spoilage microorganism isolated from food and their control in wheat bread. Food Control, 31: 539-545.
[28]  Denkova, S.; I. Z. Denkova, G. Ljubk; M. Yordanovaa; D. Nikolova and Y. Evstatieva (2014). Production of wheat bread without preservatives using sourdough starters. Biotechnology & Biotechnological Equipment. 28 (5): 889-898.
[29]  A.O.A.C. (2000). Official Methods of Analysis, 17th Ed., Association of Official Analytical Chemists International. Gaithersburg, Maryland, USA.
[30]  Oxoid Manual (1990). The Oxoid Manual of Culture, Media and Other Laboratory Services. Sixth Edition.
[31]  Plesssas, S.; A. Bekatorou; J. Gallangh; P. Nigam; A.A. Koutinas and C. Psarianos (2008). Evolution of aroma volatiles during storage of sourdough breads made by mixed cultures of Kluveromycesmarxianus and lactobacillus delbrukii ssp. Bulgaricus or lactobacillus helveticus. Food Chem., 107: 883-889.
[32]  GÜl, H.; S. Özcelik; O. SăGdic and M. Certel (2005). Sourdough bread production with Lactobacilli and S. cerevisiae isolated from sourdoughs. Process Biochem., 40: 691-687.
[33]  Robert, H.; V. Gabriel; D. Lefebvre; P. Rabier; Y. Vayssier and C. ontagné- Faucher (2006). Study of the behavior of Lactobacillus Plantarum and Leuconostoc starters during a complete wheat sour dough breadmaking process. LWT 39: 256-265.
[34]  Schillinger, V. and F. K. Lücke (1989). Antimicrobial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55: 1901-1906.
[35]  Faridi, H.A. and Rubenthalar (1984). Effect of baking time and temperature on bread quality, starch gelatinization and staling of balady bread. Cereal Chem., 61: 151-154.
[36]  Kitterman, S. and G.L. Rubanthaler (1971). Assessing the quality of orally generation wheat selection with the micro AWRC test. Cereal Sci., Today, 16: 313.
[37]  Mousa, E.I.; R.H. Ibrahim; W.C. Shuey and R.D. Maneval (1979). Influence of wheat classes, flour extractions, and baking methods on Egyptian Balady Bread. Cereal Chem., 56 (6): 563-566.
[38]  Sendecor, G.W. and Cochran, W.G. (1997).Statistical Methods; 7th Ed. Oxford and J; B.H.Publishing Co. pp. 504.
[39]  SAS (1997). Statistical Analysis System. User's Guide: Statistics, SAS Institute Inc, Gary, Nc., USA.
[40]  Ahmed, M.A.A. (2009). Role of mixed microbial cultures in improvement of bread quality. M.Sc. Thesis. Fac. of Agric. Ain Shams Univ.
[41]  Gobbetti, M.; M.S. Simonetti; A. Corsetti; F. Santinelli; J. Rossi and P. Damiani (1995). Volatile compound and organic acid productions by mixed wheat sour dough starters: influence of fermentation parameters and dynamics during baking. Food Microbiol., 12: 497-507.
[42]  Corsetti, A.; P. Lavermococca; M. Morea; F. Baruzzi; N. Tosti and M. Gobbetti (2001). Phenotypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat sourdoughs of southern Italy. Int. J. of Food Microbiol., 64: 95-104.
[43]  Yousif, M.R.G1 and S. M. Faid (2014). Effect of Using Different Types of Yeasts on the Quality of Egyptian Balady Bread. Journal of American Science 10 (2):100-109.
[44]  Wehrle, K. and E.K. Arendt (1998). Rheological changes in wheat sourdough during controlled and spontaneous fermentation. Cereal Chem., 75 (6): 882-886.
[45]  Oscroft, C.A.; J.G. Banks; and S. Mephee (1990). Inhibition of thermally-stressed bacillus spores by combinations of nisin, pH and organic acids. Lebensm. Wiss. Technol., 23 (6): 538-544. (c.f. Yousif, M.R.G. (2011). Ph.D Thesis, Fac. of Agric. Ain Shams Univ).
[46]  Ur- Rehman, S.; H. Nawaz; S. Hussain; M.M. Ahmad; M.A. Murtaza and M.S.Ahmad (2007). Effect of Sourdough Bacteria on the Quality and Shelf Life of Bread. Pakistan J. of Nutrition 6 (6): 562-565.
[47]  Rumeus, I and M. Turtoi (2013). Influence of sourdough use on rope spoilage of wheat bread. J. Agroalimentary Processes and Technol. 19(1), 94-98.
[48]  Hansen, A. and P. Schieberle (2005). Generation of aroma compounds during sourdough fermentation: Applied and fundamental aspects. Trends in Food Sci. and Technol., 16: 85-94.
[49]  Korakali, M.; M. Pavlovic; M.G. Ganzle and R.F. Vogel (2003). Exopolysaccharides and kestose production by lactobacillus sanfranciscensis LTH2590. Appl. and Environ. Microbiol. 69(4): 2073-2079.
[50]  Sadeghi, A.; F. Shahidi, A. Mortazavi and M.A. Mahallati (2008). Evaluation of lactobacillus sanfrancisco (ATCC 14917) and Lactobacillus plantarum (ATCC 43332) effects on Iranian Barbari bread shelf life. 7(18): 3346-3351.
[51]  Petrulákova, Z.; H. MikuŠová, L. Gereková; M. Kockova and E. Šturdik (2009). The effect of lactobacilli starter culture on quality of bread. Acta Chimica Slovaca. 2(2): 120-128.