Production and Estimation of Keratinase by Immobilized and Free Bacillus licheniformis (St. 24)

Farag M. Saieb¹, Salama A. Boaker¹, Hasham M. El-komy² and Ahmed Issa^{3, 4,}

¹Microbiology Laboratory, Department of Botany, Omar Almukhtar University, El-beida, Libya

²Department of Botany, Faculty of Science, Minia University, Minia, Egypt

³Biology Department, Faculty of Science, Taif University, Taif KSA

⁴Department of Botany, Faculty of Science, Assiut University, Assiut , Egypt

Cite this paper:
Farag M. Saieb, Salama A. Boaker, Hasham M. El-komy and Ahmed Issa. Production and Estimation of Keratinase by Immobilized and Free Bacillus licheniformis (St. 24). Journal of Applied & Environmental Microbiology. 2015; 3(5):119-122. doi: 10.12691/jaem-3-5-2

Abstract

Alginate immobilized Bacillus licheniformis (St. 24) were isolated from chicken feather wastes recorded higher keratinase production than free bacterial suspension. 2-3% alginate concentration, medium pellet size, and in addition of keratin powder as adjuvant were optimum for keratinase production by the immobilized bacteria. Encapsulated B. licheniformis st. 24 was successfully produced keratinase for three repeated batch fermentation cycles each 24 hour incubation period. Immobilization of the whole cells proved to be useful for continuous production of keratinase and feather degradation.

Keywords:
Bacillus licheniformis keratinase

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