Journal of Applied & Environmental Microbiology
ISSN (Print): 2373-6747 ISSN (Online): 2373-6712 Website: http://www.sciepub.com/journal/jaem Editor-in-chief: Sankar Narayan Sinha
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Journal of Applied & Environmental Microbiology. 2014, 2(2), 61-64
DOI: 10.12691/jaem-2-2-5
Open AccessArticle

Isolation of Arcobacter Butzleri from Caspian Sea’s Water

Fahimeh Ghorbani Moeinand Masood Ghane1,

1Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

Pub. Date: March 16, 2014

Cite this paper:
Fahimeh Ghorbani Moeinand Masood Ghane. Isolation of Arcobacter Butzleri from Caspian Sea’s Water. Journal of Applied & Environmental Microbiology. 2014; 2(2):61-64. doi: 10.12691/jaem-2-2-5

Abstract

The major purpose of this study was isolation, identification and characterization of Arcobacter spp. from Caspian Sea’s water in north of Iran. Sampling from the water was conducted within 4 seasons. Arcobacter spp. was isolated using standard method then identified by Phenotyping tests. Finally, the identification of strain was verified by PCR method. After conducting of the Phenotyping methods and their authentication with molecular technique, fourteen strains of Arcobacter butzleri were identified. With regard to the obtained results, prevalence of this bacterium in the coastal waters of the Caspian Sea’s was evaluated as 5.32 percent. The result obtained from this study is, in fact, regarded as the first report from the isolation of Arcobacter butzleri from Caspian Sea. Knowledge of the state of the entry and time span of the survival of the Arcobacter in the water environments to control the water quality and prevention from the disease is of importance.

Keywords:
Arcobacter butzleri isolation Caspian Sea water sample

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References:

[1]  Alispahic, M., Hummel, K., Jandreski-Cvetkovic, D., Bauer, K., “Species-specific identification and differentiation of Arcobacter, Helicobacter and Campylobacter by full-spectral matrix-associated laser esorption/ionization time of flight mass spectrometry analysis.” J. Med. Microbiol. 59. 295-301. 2010.
 
[2]  Ellis, W. A., Neill, S. D., O'Brien, J. J., Ferguson, H.W. and Hanna, J. “Isolation of Spirillum/Vibrio-like organisms from bovine fetuses.” Vet. Rec. 100. 451-452. 1977.
 
[3]  Vandamme, P., Pugina, P., Benzi, G. and van Etterijck, R. “Outbreak of recurrent abdominal cramps associated with Arcobacter butzleri in an Italian school”. Journal Clinical Microbiol. 30. 2335-7. 1992
 
[4]  ICMSF. “Microorganisms in foods. 7. Microbiological testing in food safety management”. International Commission on Microbiological Specifications for Foods. Kluwer Academic/Plenum, New York, NY. 2002.
 
[5]  Houf, K. and Stephan, R. “Isolation and characterization of the emerging foodborne pathogen Arcobacter from human stool”. Journal of Microbiological Methods. 68. 408-413. 2007.
 
[6]  Kiehlbauch, J. A., Brenner, Plikaytis, B. D., Swaminathan, B., Cameron, D. N. and Wachsmuth, I. K. “Restriction fragment length polymorphisms in the ribosomal genes for species identification and subtyping of aerotolerant Campylobacter species”. J. Clin. Microbiol. 29. 1670-1676. 1991.
 
[7]  Vandenberg, O., Dediste, A., Houf, K. and Ibekwem, S. “Arcobacter Species in Humans”. Emerging. Infectious disease, 42. 1851-1852. 2004
 
[8]  Wesley, I. V. and Miller, G. W. “Arcobacter: an opportunistic human food-borne pathogen? p. 185-211. In W. M. Scheld, M. L. Grayson, and J. M. Hughes (ed.), Emerging infections 9”. ASM Press, Washington, DC. 2010
 
[9]  Phillips, C. A. “Arcobacters as emerging human food borne pathogens”. Food Control. 12: 1-6. 2001.
 
[10]  Diergaardt S. M., Venter S. N., Spreeth A., Theron J., and Brozel V. S. “The occurrence of Campylobacters in water sources in South Africa”. Water. Res. 38. 2589-2595. 2004.
 
[11]  Moreno, Y., Alonso, J. L., Botella, S., Ferrus, M. A. and Hernandez, J. “Survival and injury of Arcobacter after artificial inoculation into drinking water”. Res. Microbiol. 155. 726-730. 2004.
 
[12]  Morita, Y., Maruyama, S., Kabeya, H., Boonmar, S., Nimsuphan, B., Nagai, A., Kozawa, K., Nakajima, T., Mikami, T., Kimura, H. “Isolation and phylogenetic analysis of Arcobacter spp. in ground chicken meat and environmental water in Japan and Thailand”. Microbiol. Immunol. 48: 527-533. 2004.
 
[13]  Rice, E. W., Rodgers, M. R., Wesley, I. V., C. H. Johnson, and S. A. Tanner. “Isolation of Arcobacter butzleri from ground water”. Lett. Appl. Microbiol. 28. 31-35. 1999.
 
[14]  Keller, S., Räber, S., Tasara, T. and Stephan, R. “Prevalence of Arcobacter butzleri in fecal samples, on carcasses and in retail meat of cattle, pig and poultry in Switzerland”. Archiv für Lebensmittelhygiene. 57. 64-68. 2006.
 
[15]  Sette, L. D., Simioni, K. C., Vasconcellos, S. P., Dussan, L. J., Neto, E. Y. and Oliveira, V. M. “Analysis of the composition of bacterial communities in oil reservoirs from a southern offshore Brazilian basin”. Antonie Leeuwenboek. 91. 253-266. 2007.
 
[16]  Moussard, H., Corre, E., Cambon-Bonavita, M. A., Fouquet, Y. and Jeanthon, C. “Novel uncultured Epsilonproteobacteria dominate a filamentous sulphur mat from the 13 degrees N hydrothermal vent field, East Pacific Rice”. FEMS Microbiol. Ecol. 58: 449-463. 2006.
 
[17]  Debruyne, L., Gevers, D. and Vandamme, P. “Taxonomy of the family Campylobactereaceae. p. 3-25. In I. Nachamkin, C. Szymanski, and M. Blaser (ed.), Campylobacter, 3rd ed.” ASM Press, Washington, DC. 2008.
 
[18]  Atabay, H.I. and Corry, J.E.I. “The prevalence of campylobacters and arcobacters in broiler chickens”. Journal of Applied Microbiology. 83. 619-626. 1997.
 
[19]  Snelling, W. J., Matsuda, M., Moore, J. E. and Dooley, J. S. “ Under the microscope” Arcobacter. Lett. Appl. Microbiol. 42: 7-14. 2006.
 
[20]  Figueras, M. J., Collado, L. and Guarro, J. “A new 16S rDNA-RFLP method for the discrimination of the accepted species of Arcobacter”. Diagn. Microbiol. Infect. Dis. 62. 11-15. 2008.
 
[21]  On, S. L., Holmes, B., and Sackin, M. J. “A probability matrix for the identification of campylobacters, helicobacters and allied taxa.” J. Appl. Bacteriol. 81. 425-432. 1996.
 
[22]  Abdelbaqi, K., et al. “Development of a real-time fluorescence resonance energy transfer PCR to detect Arcobacter species”. J. Clin. Microbiol. 45. 3015 3021. 2007.
 
[23]  Kopilovic, B., Ucakar, V., Koren, N., Krek, M. and Kraigher, A. “Waterborne outbreak of acute gastroenteritis in a coastal area in Slovenia in June and July 2008”. Eurosurveillance. 13. 1-3. 2008.
 
[24]  Bu¨cker, R., Troeger, H., Kleer, J., Fromm, M. and Schulzke, J. D. “Arcobacter butzleri induces barrier dysfunction in intestinal HT-29/B6cells.” J. Infect. Dis. 200. 756-764. 2009.
 
[25]  Fera, M. T., Maugeri, T. L., Gugliandolo, C., Beninati, C. “Detection of Arcobacter spp. in the coastal environment of the Mediterranean Sea”. Appl. Environ. Microbiol. 70. 1271-1276. 2004.
 
[26]  Collado, L., Inza, I., Guarro, J. Guarro, and Figueras, M. J. “Presence of Arcobacter spp. in environmental waters correlates with high levels of fecal pollution”. Environ. Microbiol. 10: 1635-1640. 2008.
 
[27]  Chinivasagam, H. N., Corney, B. G., Wright, L. L., Diallo, I. S. and Blackall, P. J. “Detection of Arcobacter spp. in piggery effluent and effluentirrigated soils in southeast Queensland”. J. Appl. Microbiol. 103: 418-426. 2007.
 
[28]  Van Driessche, E. and Houf, K. “Survival capacity in water of Arcobacter species under different temperature conditions”. J. Appl. Microbiol. 105: 443-451. 2008.
 
[29]  Dhamabutra, N., Kamol-Rathanakul, P. and Pienthaweechai, K. “Isolation of campylobacters from the canals of Bangkok metropolitan area”. J. Med. Assoc. Thai. 75: 350-364. 1992.
 
[30]  Fera, M. T., Maugeri, T. L., Giannone, M., Gugliandolo, C., La Camera, E., Blandino, G. and Carbone, M. “In vitro susceptibility of Arcobacter butzleri and Arcobacter cryaerophilus to differentantimicrobial agents”. Int. J. Antimicrob. Agents. 21: 488-491. 2003.
 
[31]  Maugeri, T. L., Irrenra, G. P., Lentini, V., Carbone, M., Fera, M. T., G., and Gugliandolo, C. “Detection and enumeration of Arcobacter spp. Inthe Coastal environment of the Straits of Messina (Italy).” New microbial. Apr. 28 (2): 177-82. 2005.
 
[32]  Collado, L., et al. “Occurrence and diversity of Arcobacter spp. alongLlobregat river catchment, at sewage effluents and in a drinking watertreatment plant”. Water Res. 44: 3696-3702. 2010.