International Journal of Environmental Bioremediation & Biodegradation
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International Journal of Environmental Bioremediation & Biodegradation. 2018, 6(2), 36-56
DOI: 10.12691/ijebb-6-2-1
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Polycyclic Aromatic Hydrocarbons (PAHs) Metabolism by White Rot Fungi Agaricomycetes sp AGAT and Its Microcosm Study

Archana Pandey1 and Akshaya Gupte1,

1Natubhai V Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, 388 120

Pub. Date: September 11, 2018

Cite this paper:
Archana Pandey and Akshaya Gupte. Polycyclic Aromatic Hydrocarbons (PAHs) Metabolism by White Rot Fungi Agaricomycetes sp AGAT and Its Microcosm Study. International Journal of Environmental Bioremediation & Biodegradation. 2018; 6(2):36-56. doi: 10.12691/ijebb-6-2-1


Hydrocarbon pollution is a perennial problem not only in India but throughout the globe. A plethora of microorganisms have been reported to be efficient degraders of these recalcitrant pollutants. In the present study the Basidiomycetous a fungal isolate Agaricomycetes sp. AGAT was isolated from wood bark collected from Anand, Gujarat India. The isolate was screened for production of ligninolytic enzyme by primary and secondary screening. The isolate Agaricomycetes sp. AGAT is also able to degrade the polycyclic aromatic hydrocarbons (PAHs) present in the contaminated soil. The basidiomycetous fungal Agaricomycetes sp. AGAT was able to grow on 100 ppm Pyrene, Phenanthrene, Fluorene and Fluoranthene as a sole source of carbon in minimal medium. Maximum degradation of 78.53%, 85.05%, 20.5% and 70.49% of Phenanthrene, Pyrene, Fluoranthene and Fluorene respectively. The depletion in the residual PAHs in the culture medium was determined by HPLC. By GC-MS analysis metabolites were identified as 1-hydroxypyrene and phthalic acid from Pyrene, 9H- Fluoren-9-ol from Fluorene, Fluorene, 4-[1,2-dihydroxethyl] from Fluoranthene and 9,10-dihydro-9,10-dihydroxyphenanthrene from Phenanthrene. Further effect of surfactants, mediator were also studied. Phytotoxicity study of degraded metabolite was also studied.

Agaricomycetes sp. AGAT polycyclic aromatic hydrocarbons (PAHs) phytotoxicity

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[1]  Lee H, Jang Y, Choi YS, Kim MJ, Lee J, Lee H, Hong JH, Lee YM, Kim GH, Kim JJ (2014). Biotechnological procedures to select white rot fungi for the degradation of PAHs. J. Microbiol. Methods 97: 56-62.
[2]  Zhang S, Ning Y, Zhang X, Zhao Y, Yang X, Wu K, Yang S, La G, Sun X, Li X (2015). Contrasting characteristics of anthracene and pyrene degradation by wood rot fungus Pycnoporus sanguineus H1. Int. Biodeterior. Biodegradation 105: 228-232.
[3]  Wu YR, Luo ZH, Vrijmoed LLP (2010). Biodegradation of anthracene and benz[a]anthracene by two Fusarium solani strains isolated from mangrove sediments. Bioresour Technol 101: 9666-9672.
[4]  Gogoi BK, Dutta NN, Goswami P, Krishna MTR (2003) A case study of bioremediation of petroleum-hydrocarbon contaminated soil at a crude oil spill site. Adv Environ Res 7: 767-782.
[5]  Townsend GT, Prince RC, Suflita JM (2004) Anaerobic biodegradation of alicyclic constituents of gasoline and natural gas condensate by bacteria from an anoxic aquifer. FEMS Microbiol Ecol 49: 129-135.
[6]  Pham V H T, Chaudhary DK, Jeong SW, Kim J (2018).Oil-degrading properties of a psychrotolerant bacterial strain Rhodococcus sp. Y2-2, in liquid and soil media. World J Microbiol Biotechnol 34(33) 1-11.
[7]  Bamforth SM and Singleton I. (2005). Bioremediation of polycyclic aromatic hydrocarbons. J. Chem. Technol. Biotechnol. 80: 723-736.
[8]  Li XZ, Lin XG, Yin R, Wu YC, Chu HY, Zeng J, Yang T (2010). Optimization of laccase-mediated benzo[a]pyrene oxidation and the biomedical application n aged polycyclic aromatic hydrocarbons-contaminated soil. J Health Sci 56: 534-540.
[9]  Covino S, Svobodova K, Kresinova Z, Petruccioli M, Federici F, Annibale AD, Cvancarova M, Cajthaml T (2010). Invivo and in-vitro polycyclic aromatic hydrocarbons degradation by Lentinus (Panus) tigrinus CBS 577.79. Bioresour Technol 101: 3004-3012.
[10]  Han Mj, Choi HT, Song HG (2004). Degradation of Phenanthrene by Trametes versicolor and its laccase. J Microbiol42(2): 94-98.
[11]  Pozdnyakova N, Rodakiewicz j, NowakTurkovskaya OV, Haber J. (2006). Oxidative degradation of polyaromatic hydrocarbons catalyzed by blue laccase form Pleurotus ostreatus D1 in the presence of synthetic mediators. Enzyme Microb. Technol. 39: 1242-1249.
[12]  Wilson SC and Jones KC. (1993). Bioremediation of soil contaminated with polynuclear aromatic hydrocarbons (PAHs): A review. Environ Pollut 81(3): 229-49.
[13]  Johnsy G and Kaviyarasan V. (2014). Effect of physico-chemical parameters on ligninolytic enzyme production of an indigenous isolate of Neolentinus kanffmanii under submerged condition. World J Pharm Sci 2321-3310.
[14]  Tien M and Kirk TK. (1988). Lignin peroxidase of Phanerochaete chrysosporiumMethods Enzymol l161:238-249.
[15]  Kalme SD, Jadhav SU, Parshetti GK, Govindwar SP (2010). Biodegradation of Green HE4B: Co-substrate effect, biotransformation enzymes and metabolite toxicity analysis. Indian J Microbiol. 50: 156-154.
[16]  Chhaya U and Gupte A (2013). Effect of different cultivation conditions and inducers on the production of Laccase by the litter-dwelling fungal isolate Fusariumin carnatum LD-3 under solid state fermentation, Ann Microbiol 63(1): 215-223.
[17]  Gao H, Wang Y, Zhang W, Wang W, Mu Z. (2011). Isolation, identification and application in lignin degradation of ascomycetes GHJ-4. Afr. J. Biotechnol.. 10: 4166-4174.
[18]  Patel H, Gupte A, Gupte S. (2009). Biodegradation of Fluoranthene by basidiomycetes fungal isolate Pleurotus ostreatus HP-1 Appl Biochem Biotechnol 157: 367-376.
[19]  Agrawal N, Verma P, Singh RS, Shahi SK (2017). Ligninolytic enzyme production by white rot fungi Podoscypha elegans strain FTG4. International Journal of Current Microbiology and Applied Sciences 6(5): 2757-2764.
[20]  Asther M, Lesage L, Drapron R, Corrieu G, Odie RE. (1988). Phospholipid and fatty acid enhancement of Phanerochaete chrysoporium INA - 12 in relation to ligninase production. Appl Microbiol Biotechnol. 27: 393-398.
[21]  Vares T, Kalsi M, Hatakka A. (1995). Lignin peroxidases, manganese peroxidases, and other ligninolytic enzymes produced by Phlebia radiata during solid-state fermentation of wheat straw. Appl Environ Microbiol 61: 351-3520.
[22]  Gupte A, Gupte S and Patel H (2007). Ligninolytic enzyme production under solid state fermentation by white-rot fungi. Journal of Scientific and Industrial Research. 66: 611-614.
[23]  Kapoor S, Khanna PK, Katyal P. (2009). Effect of supplementation of wheat straw on growth and lignocellulolytic enzyme potential of Lentinus edodes. World Journal of Agricultural Sciences 5: 328-331.
[24]  Hadibarata T and Tachibana S (2010). Characterization of Phenanthrene degradation by strain Polyporus sp. S133. J Environ Sci 22(1): 142-149.
[25]  Wirasnita R and Hadibarata T (2016). Potential of the White-Rot Fungus Pleurotus pulmonarius F043 for Degradation and Transformation of Fluoranthene. Pedosphere 26(1): 49-54.
[26]  Bezalel L, Hadar Y, Cerniglia C (1997). Enzymatic Mechanism Involved in Phenanthrene Degradation by the White Rot Fungus Pleurotus ostreatus. Appl Environ Microbiol 63(7): 2495-2501.
[27]  Hadibarata T and Kristanti R.A (2014). Potential of a White-rot fungus Pleurotus eryngii F032 for degradation and transformation of fluorene. Fungal Biol. 118: 222-227.
[28]  Mao J and Guan W (2016). Fungal degradation of polycyclic aromatic hydrocarbon (PAHs) by Scopulariopsis brevicaulis and its application in bioremediation of PAH-contaminated soil. ACTA Agricultuare Scandinavica Section B-Soil and Plant Science 66(5): 399-405.
[29]  Hammel KE, Gai WZ, Green B, Moen MA (1922). Oxidative Degradation of Phenanthrene by the Ligninolytic Fungus Phanerochaete chrysosporium. Appl Environ Microbiol 58(6): 1832-1838.
[30]  Hwang HM, Hu X, Zhao X (2007). Enhanced bioremediation of polycyclic aromatic hydrocarbons by environmentally friendly techniques. J Environ Sci Health 25: 313-352.
[31]  Tekere M, Read JS, Mattiasson B (2005). Polycyclic aromatic hydrocarbon biodegradation in extracellular fluids and static batch cultures of selected sub-tropical white rot fungi. J Biotechnol 115: 367-377.
[32]  Wang N, Hill G, Peng J (2002). The role of glucose in developing enhanced biological phosphorous removal. Environmental Engineering and Policy 3: 45-54.
[33]  Swamy J and Ramsay JA (1999). Effects of glucose and NH4+ concentration on sequential dye decolorization by Trametes versicolor. Enzyme Microb Technol 25: 278-284.
[34]  Reddy PG, Singh HD, Roy Pk, Baruah JN (1982). Predominant role of hydrocarbon solubilization in the microbial uptake of hydrocarbons. Biotechnol. Bioeng. 24: 1241-1269.
[35]  Cerniglia CE. (1992).Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation 3(2): 51-368.
[36]  Tiehm A. (1994). Degradation of polycyclic aromatic hydrocarbons in the presence of synthetic surfactants. Appl Environ Microbiol 60: 258-263.
[37]  Hadibarata T, Tachibana S, Itoh K.(2009). Biodegradation of chrysene, an aromatic hydrocarbon by Polyporus sp. S133 in liquid medium. J Hazard Mater164: 911-917.
[38]  Zhao H, Wu Q, Wang L, Zhao X, Gao H. (2009). Degradation of Phenanthrene by bacterial strain isolated from soil in refinery fields in Shanghai China. J Hazard Mater 164: 863-869.
[39]  Garon D, Krivobok S, Wouessidjewe D,Seigle-Murandi F. (2002). Influence of surfactants on solubilization and fungal degradation of fluorine. Chemosphere 47: 303-309.
[40]  Balaji V, Arulazhagan P, Ebnezer P. (2014). Enzymatic bioremediation of polyaromatic hydrocarbons by fungal consortia enriched from petroleum contaminated soil and oil seeds. J Env Biol 35: 521-529.
[41]  Majcherczyk A, Johannes C, Huttermann A (1988). Oxidation of polycyclic aromatic hydrocarbon (PAH) by laccase of Trametes versicolor. Enzyme Microb. Technol. 22: 335-341.
[42]  Johannes C and Majcherczyk (2000). Natural Mediators in the oxidation of Polycyclic Aromatic Hydrocarbons by Laccase Mediator System. Appl Environ Microbiol 66(2): 524-528.
[43]  Kastner M, Breuer-Jammali M, Mahro B (1998). Impact of inoculation protocols, salinity and pH on the degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of PAH-degrading bacteria introduced into soil. Appl Environ Microbiol 64: 359-362.
[44]  Patel V, Patel J, Madamwar D (2013). Biodegradation of Phenanthrene in bio augmented microcosm by consortium Asp developed from coastal sediment of Alang-Sosiya ship breaking yard. Marine Poll Bull 74: 199-207.
[45]  Teng Y, Luo Y, Sun M, Liu Z, Li Z, Christie P (2010). Effect of bioaugmentation by Paracoccus sp strain HPD-2 on the soil microbial community and removal of polycyclic aromatic hydrocarbons from an aged contaminated soil. Bioresour Technol 101: 3437-3443.
[46]  Jain PK and Bajpaiv (2012). Biotechnology of Bioremediation- An review. International Journal of Environmental Science 3: 535-549.
[47]  Singh A and Ward OP (2004). Biotechnology and Bioremediation - an overview. Biodegradation and Bioremediation. Springer 1-17.
[48]  Winquist E, Bjorklof K, Schultz E, Rasanen M, Salonen K, Anasonye F, Cajthaml T, Steffen K, Jorgensen K, Tuomela M. (2016).Bioremediation of PAH-contaminated soil with fungi- From laboratory to field scale. Int. Biodeterior. Biodegradation 86: 238-247.
[49]  Acevedo F, Pizzul L, Castillo MD, Cuevas R, Diez MC (2011). Degradation of polycyclic aromatic hydrocarbons by the Chilean white-rot fungus Anthracophyllum discolor. J. Hazard. Mater. 185: 212-219.
[50]  Bezalel L,Hadar Y,Fu PP, Freeman JP, Cerniglia C (1996). Metabolism of Phenanthrene by white rot fungus Pleurotus ostreatus .Applied And Environmental Microbiology 62(7): 2547-2553.
[51]  Sack U, Heinze TM, and Deck J, et al. (1997). Comparison of Phenanthrene and pyrene degradation by different wood-decaying fungi. Appl Environ Microbiol 63: 3919-3925.
[52]  Vila J, Lopez Z, Sabate J, Minguillon C, Solanas AM, Grifoll M (2001). Identification of a novel Metabolite in the Degradation of Pyrene by Mycobacterium sp. Strain AP1: Actions of the Isolate on Two-and Three-Ring Polycyclic Aromatic Hydrocarbons. Appl Environ Microbiol 67(12): 5497-5505.
[53]  Kottermann MJJ, Rietberg HJ, Hage A, Field JA. (1997). Polycyclic aromatic hydrocarbon oxidation by the white-rot fungus Bjerkandera sp. Strain BOS55 in the presence of non-ionic surfactants. Biotechnol. Bioeng. 57: 220-227.
[54]  Calvelo, Pereira R, Monterroso C, Macias F. (2010). Phytotoxicity of Hexachlorocyclohexane: Effect on Germination and Early Growth of Different Plant Species, Chemosphere 79: 326-333.
[55]  Somtrakoon K and Chouychai (2013). Phytotoxicity of single and combined polycyclic aromatic hydrocarbons toward economic crops. Russ. J. Plant Physiol. 60(1): 139-148.