International Journal of Environmental Bioremediation & Biodegradation
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International Journal of Environmental Bioremediation & Biodegradation. 2016, 4(2), 35-46
DOI: 10.12691/ijebb-4-2-2
Open AccessArticle

Decolorization of Xenobiotic Azo Dye- Black WNN by Immobilized Paenibacillus alvei MTCC 10625

Anamika Pokharia1 and Sarabjeet Singh Ahluwalia1,

1Department of Biotechnology, General Shivdev Singh Diwan Gurbachan Singh Khalsa College, Patiala -147004, Punjab, India

Pub. Date: July 08, 2016

Cite this paper:
Anamika Pokharia and Sarabjeet Singh Ahluwalia. Decolorization of Xenobiotic Azo Dye- Black WNN by Immobilized Paenibacillus alvei MTCC 10625. International Journal of Environmental Bioremediation & Biodegradation. 2016; 4(2):35-46. doi: 10.12691/ijebb-4-2-2


The present study, Paenibacillus alvei MTCC 10625 elucidates the ability for the decolorization of synthetic dye effluent containing Black WNN dye, isolated from textile wastewater contaminated site. 96.4% decolorization of Black WNN (100 mg/l) was attained at pH 9, temperature 35°C within 48 h under optimized condition with free cells of P. alvei MTCC 10625. Moreover, P. alvei MTCC 10625 immobilized over polyurethane foam (PUF) and nylon mesh (NM), achieved 95.2 and 97.0% decolorization under microaerophilic conditions within 24 h. Biodegradation analysis by FTIR and HPTLC confirms the degradation of Black WNN dye and formation of different intermediate metabolites. Further, microbial toxicity as well as phytotoxicity assay confirms the nontoxic nature of the effluent released from the outlet of column. The former is in relation to using soil microbial flora; Bacillus cereus, Bacillus subtilis and Kocuria rosea MTCC 5932; whereas the later by using Triticum aestivum and Vigna radiata plants.

biodegradation black WNN dye decolorization nylon mesh polyurethane foam

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