International Journal of Environmental Bioremediation & Biodegradation
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International Journal of Environmental Bioremediation & Biodegradation. 2016, 4(1), 1-7
DOI: 10.12691/ijebb-4-1-1
Open AccessArticle

Presence of Arbuscular Mycorrhizal Fungi on Fern from Tailing Deposition Area of Gold Mine in Timika, Indonesia

Suharno1, , Rina Sri Kasiamdari2, Endang Sutariningsih Soetarto3 and Retno Peni Sancayaningsih4

1Department of Biology, Faculty of Mathematics and Natural Science, University of Cenderawasih, Jayapura, Papua, Indonesia

2Laboratory of Plant Taxonomy, Faculty of Biology, Gadjah Mada University, Yogyakarta

3Laboratory of Microbiology, Faculty of Biology, Gadjah Mada University, Yogyakarta

4Laboratory of Plant Ecology, Faculty of Biology, Gadjah Mada University, Yogyakarta

Pub. Date: January 25, 2016

Cite this paper:
Suharno, Rina Sri Kasiamdari, Endang Sutariningsih Soetarto and Retno Peni Sancayaningsih. Presence of Arbuscular Mycorrhizal Fungi on Fern from Tailing Deposition Area of Gold Mine in Timika, Indonesia. International Journal of Environmental Bioremediation & Biodegradation. 2016; 4(1):1-7. doi: 10.12691/ijebb-4-1-1

Abstract

Detection of the presence of arbuscular mycorrhizal fungi (AMF) on marginal land within degrading reclamation effort land is very important to support the success of such reclamation. The purpose of this study is to determine the presence of AMF in the root rhizosphere of fern plants Equisetum debile, Pteris radicans, and Nephrolepis hirsutula that grow in the tailing area of gold mine Timika. The study used a survey method to isolate AMF from the root rhizosphere of fern plants in the gold mine tailing deposition area of the Modified Ajkwa Deposition Area (ModADA) of PT Freeport Indonesia (PTFI) in Mimika Regency, Papua, Indonesia. AMF infection was tested using a trypan blue staining method, while the calculation of the percentage of infection was carried out by a slide method. The presence of AMF spores was detected by a wet sieving method. The results of the study showed that there were AMFs in the root rhizosphere of the three types of fern. However, based on the observation of infection of the plant root system, there were only P. radicans indicating to interact with AMF, while E. debile and N. hirsutula were not associated with AMF. There were 54. 44% of infection at the root rhizosphere found in P. radicans and number of spores ranged from 8 to 12 per 10 g of soil samples. Although there were no infection of AMF on the root rhizosphere of E. debile and N. hirsutula, however there were 4.33 and 11 spores per 10 g of soil samples of both plant rhizosphere respectively. Based on morphology, it can be identified that the types of AMF were member of genus Scutelospora, Glomus, Claroideoglomus, and Acaulospora.

Keywords:
Arbuscular Mycorrhizal Fungi Tailings Ferns Gold Mine Timika

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