International Journal of Environmental Bioremediation & Biodegradation
ISSN (Print): 2333-8628 ISSN (Online): 2333-8636 Website: http://www.sciepub.com/journal/ijebb Editor-in-chief: Apply for this position
Open Access
Journal Browser
Go
International Journal of Environmental Bioremediation & Biodegradation. 2015, 3(2), 48-53
DOI: 10.12691/ijebb-3-2-2
Open AccessArticle

Enhanced Aerobic Biodegradation of Naphthalene in Soil: Kinetic Modelling and Half-Life Study

S. E. Agarry1, and K. M. Oghenejoboh1

1Biochemical and Bioenvironmental Engineering Laboratory, Department of Chemical Engineering, Delta State University, Oleh Campus, Nigeria

Pub. Date: June 07, 2015

Cite this paper:
S. E. Agarry and K. M. Oghenejoboh. Enhanced Aerobic Biodegradation of Naphthalene in Soil: Kinetic Modelling and Half-Life Study. International Journal of Environmental Bioremediation & Biodegradation. 2015; 3(2):48-53. doi: 10.12691/ijebb-3-2-2

Abstract

To demonstrate the potential use of bioremediation in polycyclic aromatic hydrocarbons contaminated soil using naphthalene as a model pollutant, a laboratory study with the objectives of investigating, evaluating and comparing the methods of natural attenuation, biostimulation, bioaugmentation, and combined biostimulation and bioaugmentation was performed. The study dealt with naphthalene biodegradation in soil using inorganic NPK fertilizer and mixed culture of Alcaligenes, Aeromonas, Micrococcus, and Serratia as source of biostimulation and bioaugmentation, respectively. Each treatment strategy contained 4% (w/w) naphthalene in soil as a sole source of carbon and energy. After 4 weeks of remediation, the results revealed that natural attenuation, biostimulation, bioaugmentation, and combined biostimulation and bioaugmentation exhibited 44%, 69.5%, 77.5%, and 85% naphthalene degradation, respectively. Also, the total hydrocarbon-degrading bacteria (THDB) count in all the treatments increased throughout the remediation period. The highest bacterial growth was observed for combined biostimulation and bioaugmentation treatment strategy. A first-order kinetic model was fitted to the biodegradation data to evaluate the biodegradation rate and the corresponding half-life time was estimated. The model revealed that naphthalene contaminated-soil microcosms under combined biostimulation and bioaugmentation treatment strategy had higher biodegradation rate constants, as well as lower half-life times, than other remediation systems. Therefore, the kinetic parameter values showed that the degree of effectiveness of these bioremediation strategies in the cleanup of naphthalene contaminated soil is in the following order: natural attenuation < biostimulation < bioaugmentation < combined biostimulation and bioaugmentation. Thus, the present work will contribute to the development of strategies for in situ treatment of polycyclic aromatic hydrocarbons contaminated soils.

Keywords:
bioremediation biostimulation bioaugmentation first-order kinetics naphthalene

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Bisht, S., Pandey, P., Sood, A., Sharma, S., Bisht, N.S. “Biodegradation of naphthalene and anthracene by chemo-tactically active rhizobacteria of populus deltoids. Brazilian Journal of Microbiology 41: 922-930, 2010.
 
[2]  Miya, R.K., Firestone, M.K. “Phenanthrene-degrader community dynamics in rhizosphere soil from a common annual grass”. Journal of Environmental Quality 29: 584-592, 2000.
 
[3]  Directive 2000/60/EC, 2000. EU Water Framework Directive. Official Journal L 327.
 
[4]  U.S. Environmental Protection Agency Technical Background Document to Support Rulemaking Pursuant to the Clean Air Act - Section 112(g). Ranking of Pollutants with Respect to Hazard to Human. EPA-450/3-92-010.Emissions Standards Division, Office of Air Quality Planning and Standards, Research Triangle Park, NC.1994.
 
[5]  Mueller J. G., Chapman P.J., Pritchard P.H. “Creosote-contaminated sites”. Environmental Science and Technology 23: 1197-1202, 1989.
 
[6]  Siciliano, S.D., Germida, J.J., Banks, K., Greer, C.W. “Changes in microbial community composition and function during a polyaromatic hydrocarbon phytoremediation field trial”. Applied Environmental Microbiology 69: 483-489, 2003.
 
[7]  Agency for Toxic Substances and Disease Registry (ATSDR), Toxicological Profile for Naphthalene and 2-Methylnaphthalene. U.S. Public Health Service, U.S. Department of Health and Human Services, Atlanta, GA, 1990.
 
[8]  Amoore J. E., Hautala E. “Odor as an aid to chemical safety: Odor thresholds compared with threshold limit values and volatilities for 214 industrial chemicals in air and water dilution”. Journal of Applied Toxicology 3: 272-290, 1983.
 
[9]  Grund, Denecke B, Eichenlab “Naphthalene degradation via salicylate and gentisate by Rhodococcus spp.strain B4” Applied Environmental Microbiology 55:1874-1877, 1992.
 
[10]  Karl J Rockne, Joanne C Chee-Sanford, Robert A Sanford, Brian P Hedlund, James T Stanley, et al. “Anaerobic Naphthalene Degradation by Microbial Pure Cultures under Nitrate-Reducing Conditions”. Applied and Environmental Microbiology 66: 1595-1601, 2000.
 
[11]  Sukor, M.Z., Yin, C-Y, Savory, R.M., Abdul-Talib, S. “Biodegradation kinetics of naphthalene in soil medium using Pleurotus ostreatus in batch mode with addition of fibrous biomass as a nutrient”. Bioremediation Journal, 16 (3): 177-184, 2012.
 
[12]  Pawar AN, Ugale SS, More MG, Kokani NF, Khandelwal SR “Biological Degradation of Naphthalene: A New Era”. Journal of Bioremediation and Biodegradation 4: 203.2013
 
[13]  Bach, Q.D., Kim, S.J., Oh, Y.S. “Enhancing the intrinsic bioremediation of PAH-contaminated anoxic estuarine sediments with biostimulating agents”. Journal of Microbiology 43(4): 319-324, 2005.
 
[14]  Owabor, C.N., Ogunbor, O.F. “Naphthalene and pyrene degradation in contaminated soil as a function of the variation of particle size and percent organic matter”. African Journal of Biotechnology 6(4): 436-440, 2007.
 
[15]  Agarry, S. E., Yusuf, R. O., Ajani, A. O. “Biodegradation of anthracene and naphthalene in soil”. Journal of Nigerian Society of Chemical Engineers, 25 (1-2): 55-63, 2010a.
 
[16]  Agarry, S.E., Owabor, C.N. “Anaerobic bioremediation of marine sediment artificially contaminated with anthracene and naphthalene”. Environmental Technology, 32(12): 1375-1381, 2011.
 
[17]  Kanaly, R.A., Harayahama, S. (2000) Biodegradation of high molecular weight polycyclicaromatic hydrocarbons by bacteria. J. Bacteriol. 182: 2059-2067.
 
[18]  Han, M.j., Choi, H.T., Song, H.G. “Degradation of phenanthrene by Trametes versicolor and its laccase”. Journal of Microbiology 42: 94-98, 2003.
 
[19]  Thomas, S.P., Stensel, H.D., Strand, S.E. “Biodegradation of polyaromatic hydrocarbons by marine bacteria Effect of solid phase on degradation kinetics”. Water Research. 33: 868-880, 1998.
 
[20]  Elliot, M. “Polycyclic aromatic hydrocarbons and redox parameter in a creosote-contaminated aquifer”. M.Sc. Thesis, Virginia Polytechnic Institute and State University, USA, 2001.
 
[21]  Wang, X.C., Zhang, Y.X., Chen, R.F. “Distribution and partitioning of polycyclic aromatic hydrocarbons (PAHs) in different size fractions in sediments from Boston Harbor, United States”, 2001.
 
[22]  Leahy, J.G., Colwell, R.R. “Microbial degradation of hydrocarbons in the environment”. Microbiological Reviews 54: 305-315, 1990.
 
[23]  Thibault, S.I., Anderson, M., Frankenberger, W.T. “Influence of surfactants on pyrene desorption and degradation in soil”. Applied Environmental Microbiology 62(1): 283-287, 1996.
 
[24]  Agarry SE, Owabor CN, Yusuf RO “Enhanced bioremediation of soil artificially contaminated with kerosene: Optimization of biostimulation agents through statistical experimental design”. Journal of Petroleum and Environmental Biotechnology 3:120, 2012.
 
[25]  Vogel, T.M. “Bioaugmentation as a soil bioremediation approach”. Current Opinion in Biotechnology 7: 311-316, 1996.
 
[26]  Berardesco G, Dyhrman S, Gallagher E, Shiaris MP “Spatial and temporal variation of phenanthrene-degrading bacteriain intertidal sediments". Applied and Environmental Microbiology 64: 2560-2565, 1998.
 
[27]  McLean, E. O. Soil pH and lime requirement In “Methods in Soil Analysis: Chemical and Microbiological Properties, Part II, ed. C. A. Black. Madison, WI: American Society of Agronomy, 1982.
 
[28]  Nelson, D. W., Sommers. L.E. Determination of organic carbon. In “Method of Soil Analysis, vol. 2, ed. A. L. Page, R. H. Miller, and D. R. Keeney, 539. Madison, WI: American Society of Agronomy, 1982.
 
[29]  Bremner, J. M., Mulvaney, C.S. Total nitrogen determination. In “Method of Soil Analysis, vol. 2, ed. A. L. Page, R. H. Miller, and D. R. Keeney, pp. 595. Madison, WI: American Society of Agronomy, 1982.
 
[30]  Olsen, S. R., Sommers. L.E. Determination of available phosphorus. In “Method of Soil Analysis, vol. 2, ed. A. L. Page, R. H. Miller, and D. R. Keeney, 403. Madison, WI: American Society of Agronomy, 1982.
 
[31]  Amanchukwu, C. C., Obafemi, A., Okpokwasili, G.C. Hydrocarbon degradation and utilization by a palmwine yeast isolate. FEMS Microbiol. Lett. 57:51-54, 1989.
 
[32]  APHA “Standard Methods for the Examination of Water and Wastewater, 21st ed., American Public Health Association, Washington, DC, 2005.
 
[33]  Krieg, N. R., Holt, J. G., Sneath, P. H.A., Stanley, J. T., and Williams, S. T. “Bergey’s Manual of Determinative Bacteriology, 9th ed., Williams and Wilkins, Baltimore, 1994.
 
[34]  Agarry, S. E., Owabor, C. N., Yusuf, R. O. “Studies on biodegradation of kerosene in soil under different bioremediation strategies”. Bioremediation Journal. 14(3): 135-141, 2010b.
 
[35]  Beolchini F, Rocchetti L, Regoli F, Dell’Anno A “Bioremediation of marine sediments contaminated by hydrocarbons: Experimental analysis and kinetic modeling”. Journal of Hazardous Material 182: 403-407, 2010.
 
[36]  Olabemiwo, OM., Adediran, GO., Adekola, FA., Adelowo, OO., Olajire, AA “Biodegradation of hydrocarbon compounds in Agbabu natural bitumen”. African Journal of Biotechnology, 13 (11): 1257-1264, 2014.
 
[37]  Zhu X, Venosa AD, Suidan MT, Lee K “Guidelines for the Bioremediation of Marine Shorelines and Freshwaters Waterlands”. US Environmental Protection Agency Office of Research and Development National Risk Management Research Laboratory, 2001.
 
[38]  Wang, JL., Han, L.P., Shi, H.C., Qian, Y. Biodegradation of quinoline by gel immobilized Burkholderia sp”. Chemosphere 44:1041-1046, 2001.
 
[39]  Greene, EA., Kay, JG., Jaber, K., Stehmeier, LG., Voordouw, G “Composition of soil microbial communities enriched on a mixture of aromatic hydrocarbons”. Applied and Environmental Microbiology 66, 5282-5289, 2000.
 
[40]  Aronson, D., Boethling, R., Howard, P., Stiteler, W (2006) Estimating biodegradation half-lives for use in chemical screening. Chemosphere 63: 1953-1960 (2006).
 
[41]  Sinkkonen, S., Paasivirta, J “Degradation half-life times of PCDDs, PCDF sand PCBs for environmental fate modeling”. Chemosphere 40, 943-949, 2000.
 
[42]  Dimitrov, S., Pavlov, T., Nedelcheva, D., Reuschenbach, P., Silvani, M. et al. “A kinetic model for predicting biodegradation”. SAR QSAR Environmental Research 18, 443-457, 2007.
 
[43]  Matthies, M., Witt, J., Klasmeier, J (2008) Determination of soil biodegradation half lives from simulation testing under aerobic laboratory conditions: a kinetic model approach. Environmental Pollution 156, 99-105, 2008.
 
[44]  Zahed, MA, Abdul Aziz, H., Isa, MH, Mohajeri, L., Mohajeri, S., Kutty, SRM. “Kinetic modeling and half life study on bioremediation of crude oil dispersed by Corexit 9500”. Journal of Hazardous Materials 185, 1027-1031, 2011.
 
[45]  Agarry, SE, Aremu, MO., Aworanti, OA. (2013) Kinetic modelling and half-life study on bioremediation of soil co-contaminated with lubricating motor oil and lead using different bioremediation strategies. Soil and Sediment Contamination- An International Journal 22 (7), 800-816, 2013.
 
[46]  Yeung, PY., Johnson, RL., Xu, JG. “Biodegradation of petroleum hydrocarbons in soil as affected by heating and forced aeration”. Journal of Environmental Quality 26, 1511-1576, 1997.
 
[47]  Shabir, G., Afzal, M., Anwar, F., Tahseen, R., Khalid, Z.M. “Biodegradation of kerosene in soil by a mixed bacterial culture under different nutrient conditions”. International Journal of Biodeterioration and Biodegradation 61, 161-166, 2008.
 
[48]  Abdulsalam, S., Omale, A.B. “Comparison of biostimulation and bioaugmentation techniques for the remediation of used motor oil contaminated soil”. Brazilian Archives of Biology and Technology 52, 747-754, 2009.
 
[49]  Abdulsalam, S., Bugaje, I. M., Adefila, S. S., Ibrahim, S. (2011) Comparison of biostimulation and bioaugmentation for remediation of soil contaminated with spent motor oil. International Journal of Environmental Science and Technology 8(1), 187-194, 2011.
 
[50]  Bento, F. M., Camargo, F.A., Okeke, B., Frankenberger, Jr. T.W. (2003) Bioremediation of soil contaminated by diesel oil. Brazilian Journal of Microbiology 34(Suppl. 1), 65-68, 2003.
 
[51]  Xu, Y., Lu, M. “Bioremediation of crude oil-contaminated soil: comparison of different biostimulation and bioaugmentation treatments”. Journal of Hazardous Materials 183 (1-3), 395-401, 2010.