International Journal of Environmental Bioremediation & Biodegradation
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International Journal of Environmental Bioremediation & Biodegradation. 2014, 2(6), 250-255
DOI: 10.12691/ijebb-2-6-1
Open AccessArticle

Biodegradation and Decolorization of Reactive Dye Red ME4BL by Bacillus subtilis

Velmurugan. S1 and Ravikumar. R1,

1Department of Botany, Jamal Mohamed College, Tiruchirappalli, Tamil Nadu

Pub. Date: December 21, 2014

Cite this paper:
Velmurugan. S and Ravikumar. R. Biodegradation and Decolorization of Reactive Dye Red ME4BL by Bacillus subtilis. International Journal of Environmental Bioremediation & Biodegradation. 2014; 2(6):250-255. doi: 10.12691/ijebb-2-6-1


A bacterial strain SVM1 with remarkable ability to decolorization textile dye Reactive Red ME4BL was isolated from the activated sludge soil collected from a common effluent treatment plant (CETP) in Perunduari, Erode district Tamil Nadu. Different parameters such as temperature and pH were optimized for decolorization of Red ME4BL by using isolated bacteria. The most promising bacterial isolated was used to further dye degradation studies. The 16 s RNA gene sequencing isolated organisms as Bacillus subtilis strain SVM1 the strain showed 93% decolorization of the selected dye Reactive Red ME4BL 200mg/1000ml within 42 hours in static and shaking condition. The optimum pH and temperature for the decolorization was 8 and 37°C respectively. The biodegradation was analysis by UV-Vis, FTIR and HPLC. The result suggested that the isolated organisms Bacillus subtilis strain SVM1 can be used useful tool to treat waste water containing textile reactive dye.

Decoloriaztion Bacillus subtilis Red ME4BL dye

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[1]  Tan NCG, Borger A, Slenders P, Svitelskaya A, Lettinga G & Filed JA (2000). Degradation of azo dye mordant yellow 10 in a sequential anaerobic and bioaugmented aerobic bioreactos, water sci. Techol, 42,337-334.
[2]  Sarioglu, M., Bali, U., Bisgin, T., (2007). The removal of C.I. Basic Red 46 in a mixed methanogenic anaerobic culture. Dyes and Pigments 74, 223-229.
[3]  Pandey A., Singh P, and Iyengar L. (2007)Bacterial decolorization and degradation of azo dyes.International Biodeterioration & Biodegradation, 59:73-84.
[4]  Nozaki K., Beh C. H., Mizuno M., Isobe T., Shiroishi M. and Kanda T.(2008). Screening And Investigation Of Dye Decolorization Activities Of Basidiomycetes. Journal of Bioscience and Bioengineering, 105: 69-72.
[5]  Maulin P Shah. (2014). Microbial Degradation of Azo Dye by Pseudomonas spp 2413 Isolated from Activated Sludge of Common Effluent Treatment Plant. International Journal of Environmental Bioremediation & Biodegradation.133-138.
[6]  O Neil, C., Lopez.A., Esteves. S., Hawkes,F.R., Hawkes. D.L. and Wilcox.S. (2000). Azo dye degradation in an anaerobic – aerobic treatment system operating on simulated textile effluent. App. Microbial. Biotechnol., 53:249: 254.
[7]  Kalme, S.D., Parshetti, G.K., Jadhav, S.U., Govindwar, S.P., (2007). Biodegradation of benzidine based dye Direct Blue-6 by Pseudomonas desmolyticum NCIM 2112. Bioresource Technology 98, 1405-1410.
[8]  Manikandan N., Surumbar kuzhali and Kumuthuakalavalli R. (2012). Biodegradation of textile dye using Achromobacter xylosoxidans GRIRKNM 11 isolated from dye polluted site. J Environ and toxicol. 2161-0525.
[9]  Carliell, C.M., Barclay, S.J., Naidoo, N., Buckley, C.A., Mulholland, D.A. and Senior, E. (1995).'Microbial Decolourisationo f a ReactiveA zo Dye under Anaerobic Conditions', Water Sl, 21(l),.6r-69.
[10]  Oxspring,D. A., McMullan, G., Smyth,W. F. andM archantR. (1996).' Decolourisation and M etabolism of the Reactive Textile, Remazol Black B, by an Immobilized of Microbial Consortium', Biotechnol. Lett.,l8(5), 527-530.
[11]  Khehra MS, Saini HS, Sharma DK., Chadha BS and Chimni SS.(2006). Biodegradation of azo dye C.1 acid red88 by an anoxic- aerobic sequential bioreactor. Dyes and pigments (70): 1-7.
[12]  Salar R.K., Rohilla S.K and Rohilla J.K., (2012). Decolorization of Reactive Black HFGR by Aspergillus suphureus. Res. J. Recent Sci., 1(1), 55-61.
[13]  Aksu Z. (2005) “Application of biosorption for the removal of organic pollutants: a review” Process Biochemistry 40: 997-1026.
[14]  Olukanni S, Osuntoki OD, Gbentle AA (2006). Textile effluent biodegradation potentials of textile effluent adapted and non adapted bacteria. African J. Biotechnol., 20: 1980-1984.
[15]  Nigam, P., Mc Mullan, G., Bana! I.M. andM archant (1996).' Decolourisationo f Effluent from the Textile Industryb y a Microbial Consorsium'B, i otechnol. Lett., l8(l), l 17-I20.
[16]  Kalyani D.C, Patil P.S, Jadhav J.P, Govindwar S.P.(2008). Biodegradation of reactive textile dye Red BLI by an isolated bacterium Pseudomonas sp. SUK1. Bioresource Technology. 99: 4635-4691.
[17]  Chen, K.-C., Wu, J.-Y., Liou, D.-J., Hwang, S.-C.J., (2003). Decolorization of the textile dyes by newly isolated bacterial strains. Journal of Biotechnology 101, 57-68.
[18]  Daeshwar N, Ayazloo M, Khataee A.R, Pourhassanb M. (2007). Biological decolorization of dye solution containing malachite green by microalgae Cosmarium sp. Bioresour Technol. 98: 1176-1182.
[19]  Axelesson, J., Nilsson, U., Terrazas, E., Aliaga, T.A., Welander, U., (2006). Decolorization of the textile dyes Reactive Red 2 and Reactive Blue 4 using Bjerkandera sp. Strain BOL 13 in a continuous rotating biological contactor reactor. Enzyme and Microbial Technology 39, 32-37.
[20]  Barragan, B.E., Costa, C., Marquez, M.C., (2007). Biodegradation of azo dyes by bacteria inoculated on solid media. Dyes and Pigments 75, 73-81.
[21]  Xu, M.Y., Guo, J., Zeng, G.Q., Zhong, X.Y., Sun, G.P., (2006). Decolorization of anthraquinone dye by Shewanella decolorationis S12. Applied Microbiology and Biotechnology 71, 246-25.
[22]  Vandevivere, P.C., Bianchi, R., Verstraete,W., (1998). Treatment and reuse of wastewater from the textile wet-processing industry: review of emerging technologies. J. Chem. Technol. Biotechnol. 72, 289-302.
[23]  Jadhav J.P, Kalyani D.C, Telke A.A, Phugare S.S, Govindwar S.P. (2010). Evaluation of the efficacy of a bacterial consortium for the removal of color, reduction of heavy metals and toxicity of textile dye effluent. Bioresource Technology. 101: 165-173.
[24]  Franciscon Elisangela, Zille Andrea, Dias Guimaro Fabio, Ragagnin de Menezes Cristiano, Durrant Lucia Regina, Cavaco-Paulo Artur. (2009). Biodegradation of textile azo dyes by a facultative Staphylococcus arlettae strain VN-11 using a sequential microaerophilic/ aerobic process. International Biodeterioration & Biodegradation. 63: 280-288.
[25]  Vijaya P.P, Sandhya, S. (2003). Decolorization and complete degradation of methyl red by a mixed culture. The Environmentalist. 23: 145-149.
[26]  Sneath P.H.A., Staley J.T., Brenner DJ., Holt J.G., Castenholz R.W., Schleifer K.H., Tully J.G., Yrsing J., Williams S.T., Bryant M., Krieg N.R., Liston J., Moulder J.w., Murray R.G.E., Niven C.F. and Pfenning N. (1994). Bergey's manual of determinative bacteriology 9th edn, Williams & Wilkins.
[27]  Saratale G.D., Kalme S.D. and Govindwar S.P., (2006). Decolorization of textile dyesby Aspergillus ochraceus, Ind. J. Biotechnol. 5: 407-410.
[28]  Sheela B., Khasim beebi. S., Yellaji rao, O., (2014). Bioremediation of Ammonia using Anmonia Oxidizing Bacteria Isolated from Sewage. International Journal of Environmental Bioremediation and Biodegradation 2 (4), 146-150.
[29]  Mathew s, Madamwar D. (2004). Decolorization of Ranocid Fast Blue dye by bacterial consortium SV5. Appl Biochem Biotechnol 118: 371-381.
[30]  Leyla celik, Ayten ozturk, and Meysun I. Abudullah. (2012). Biodegradation of reactive red 195 azo dye by the bacterium Rhodopseudomonas palustris 51ATA. African journal of microbiology research 6(1), 120-126.