International Journal of Environmental Bioremediation & Biodegradation
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International Journal of Environmental Bioremediation & Biodegradation. 2014, 2(4), 146-150
DOI: 10.12691/ijebb-2-4-1
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Bioremediation of Ammonia Using Ammonia Oxidizing Bacteria Isolated from Sewage

Sheela. B1, , khasim beebi. S2 and Yellaji rao.O3

1Department of biotechnology, Gitam institute of science, GITAM University, Visakhapatnam

2Department of biotechnology, Gitam institute of Technology, Gitam University, Visakhapatnam

3Environment and safety, synergies and castings limited, Visakhapatnam

Pub. Date: June 16, 2014

Cite this paper:
Sheela. B, khasim beebi. S and Yellaji rao.O. Bioremediation of Ammonia Using Ammonia Oxidizing Bacteria Isolated from Sewage. International Journal of Environmental Bioremediation & Biodegradation. 2014; 2(4):146-150. doi: 10.12691/ijebb-2-4-1


Urbanization in the world increased the pollution levels in the environment making it for worsen life. General awareness about pollution is increasing today. To control pollution levels, biological methods are eco-safe and economical. In this study a wild strain of Bacillus species was isolated and optimized for bioremediation studies of ammonia in flask cultures. Strategy was tried to develop for ammonia bioremediation in a single stage aerobic nitrification and denitrification. The results reveal that the optimum pH for the isolated Bacillus species was 7 and optimum temperature was 30°C. At optimum pH and temperature this organism was able to remove 99.2% of ammonia in flask cultures supplemented with 5g/L of ammonium sulphate. The biomass obtained after bioremediation studies was 0.3g/L which is very low. The study on ammonia toxicity revealed that toxicity was due to pH and osmolarity.

ammonia sewage water Bacillus species growth parameters acclimatization bioremediation

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[1]  Princic.A, Mahne.I, Megasus.F, Paul.E.A, Tidje.J.M,. 1998. Effects of pH and oxygen and Ammonium concentrations on the community structure of nitrifying bacteria from wastewater. Applied and Environmental Microbiology, vol.64, No.10, pp. 3584-3590.
[2]  APHA, AWWA, WEF, 2005. Standard Methods for the Examination of Water and Wastewater. 21st Edition.
[3]  Brierley, E.D.R, Wood.M, 2001. Hetrotrophic nitrification in an acid forest soil: isolation and characterization of a nitrifying bacterium. Soil.Biol.Biochem. 33, pp.1403-1409.
[4]  Ford.D.L, 1988. Design operation and control of biological nitrification and denitrification systems. International Workshop on Wastewater Treatment Technology, Danish Association of Consulting Engineers, Copenhagen, June 11-13.
[5]  Grunditzm.C, Dalhammar.G, 2001. Development of nitrification inhibition assays using pure cultures of Nitrosomonas and Nitrobacter. Water. Res.Vol.35, No.2, pp. 433-440.
[6]  Joo.H.S, Hirai.M, Shoda.M, 2005. Nitrification and denitrification in high strength ammonium by Alcaligenes feacalis. Biotechnology letters. Vol.27 (11): 773-778.
[7]  Khin.T, Annachhatre.A.P, 2004. Nitrogen removal in a fluidized bed bioreactor by using mixed culture under oxygen limited conditions. Wat.Sci.technol, 50(6): 313-320.
[8]  Kim.D.J, Lee.D.I, Keller.J, 2006. Effect of temperature and free ammonia on nitrification and nitrite accumulation in landfill leachate and analysis of its nitrifying bacterial community by FISH. Bioresour.Technol.97, pp. 459-464.
[9]  Kim.J.K, Park.J.K, Cho.K.S, Nam.S.W, Park.T.J, Bajpai.R, 2005. Aerobic nitrification-denitrification by heterotrophic Bacillus species strains. Bio.Res.Technol. 96, pp. 1897-1906.
[10]  Leejeerajumnean.A, Ames.J.M, Owens.J.D, 2000. Effect of ammonia on the growth of Bacillus species and some other bacteria.
[11]  Muller.T, Walter.B, Wirtz.A, Barkovski.A, 2006. Ammonium toxicity in bacteria. Current microbiology. Vol.52, pp. 400-406.
[12]  Yang.X.P, Wang.S.M, Zhang.D.W, Zhou.L.X, 2011. Isolation and nitrogen removal characteristics of an aerobic heterotrophic nitrifying denitrifying bacterium, A1. China Bioresour.Technol. 102, pp. 854-862.