International Journal of Environmental Bioremediation & Biodegradation
ISSN (Print): 2333-8628 ISSN (Online): 2333-8636 Website: http://www.sciepub.com/journal/ijebb Editor-in-chief: Apply for this position
Open Access
Journal Browser
Go
International Journal of Environmental Bioremediation & Biodegradation. 2014, 2(3), 133-138
DOI: 10.12691/ijebb-2-3-6
Open AccessArticle

Microbial Degradation of Azo Dye by Pseudomonas spp 2413 Isolated from Activated Sludge of Common Effluent Treatment Plant

Maulin P Shah1,

1Industrial Waste Water Research Laboratory Division of Applied & Environmental Microbiology Enviro Technology Limited Plot No: 2413/14 GIDC, Ankleshwar-393002 Gujarat, India

Pub. Date: May 21, 2014

Cite this paper:
Maulin P Shah. Microbial Degradation of Azo Dye by Pseudomonas spp 2413 Isolated from Activated Sludge of Common Effluent Treatment Plant. International Journal of Environmental Bioremediation & Biodegradation. 2014; 2(3):133-138. doi: 10.12691/ijebb-2-3-6

Abstract

In this study we intended to isolate and identify azo dye degrading and decolorizing bacterial strains as well as investigation its biodegradation mechanism. Different bacterial isolates were isolated from activated sludge of common effluent treatment plant and tested against Reactive Violet 5 (RV5) dye. The most potent isolate was identified as Pseudomonas spp via partial sequencing of 16s rRNA DNA. The decolorized sample showed lowering of peak to a smaller absorbance value for dye concentration of 200 mg/L, which informs that the decolorization is due to removal or degradation of dye. The comparison of TLC chromatograms before and after decolorization by Pseudomonas spp under UV light showed that the original dye was quite different from the supernatant obtained after dye decolorization, which was suggested by different values of retention factors obtained in the TLC experiment This difference confirms that decolorization was due to breakdown of dyes into unknown products. The identification of several degradation products from purified RV5 by Pseudomonas spp was achieved with FTIR spectroscopy, 1H NMR and GC-MS. The results showed four compounds, nitrobenzene, 4-nitrophenol, 4-nitroaniline, and 4-nitroanisole, as degradation products.

Keywords:
pseudomonas Decolorization GC-MS FTIR reactive violet

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  P. Kaushik, A. Malik, “Fungal dye decolourization: recent advances and future potential”, Environment International, Vol. 35, No. 1, pp. 127-141, 2009.
 
[2]  K. Chen, J. Wu, D. Liu, S. J. Hwang, “Decolorization of the textile dyes by newly isolated bacterial strains”, Journal of Biotechnology, Vol. 101, No. 1, pp. 57-68, 2003.
 
[3]  A. Srinivasan, T. Viraraghavan, “Decolorization of dye waste waters by biosorbents: a review”, Journal of Environmental Management, Vol. 91, No. 10, pp. 1915-1929, 2010.
 
[4]  I. M. Banat, P. Nigam, D. Singh, R. Marchant, “Microbial decolorization of textile dye containing effluents: a review”, Bioresource Technology, Vol. 58, No. 3, pp. 217-227,1996.
 
[5]  E. Forgacs, T. Cserhati, G. Oros, “Removal of synthetic dyes from wastewaters: a review”, Environment International, Vol. 30, No. 7, pp. 953-971, 2004.
 
[6]  J. S. Knapp, P. S. Newby, “The decolourisation of a chemical industry effluent by white rot fungi”, Water Research, Vol. 33, No. 2, pp. 575-577, 1999.
 
[7]  Call, H. P. and Mucke, I. 1997. Minireview: history, overview and applications of mediated ligninolytic systems, especially laccasemediator- systems (Lignozym-Process). Journal of Biotechnology, 53, 163-202.
 
[8]  Gomare, S.S., and Govindwar, S.P. 2007. Biotransformation of the Reactive Yellow 84 by Brevibacillus laterosporus MTCC 2298. Presented at National Conference on “Emerging trends in Biotechnology for modern era”. Organised by Dept. of biotechnology, College of Computer Science and information Technology, latur, September 29-30.
 
[9]  Goszczynski, S., Paszczynski, A., Pasti-Grigsby, M.B., Crawford, R.L. 1994. New pathway for degradation of sulfonated azo dyes by microbial peroxidases of Phanerochaete chrysosporium and Streptomyces chromofuscus. Journal of Bacteriology, 176, 1339-1347.
 
[10]  Kalyani DC, Patil PS, Jadhav JP, Govindwar SP. 2008. Bio -degradation of reactive textile dye red BLI by an isolated bacterium Pseudomonas sp. SUK1. Bioresource Technol., 9: 4635-4641.
 
[11]  Krieg, N.R., and Holt, J.G. 1984. “Bergey’s Manual of Systematic Bacteriology.” vol. 1, Williams & Wilkins Co., Baltimore, pp. 161-172.
 
[12]  Manivannan, M., Reetha, D., Ganesh, P. 2011. Decolourization of Textile Azo Dyes by using Bacteria Isolated from Textile Dye Effluent. J Ecobiotech 3 (8): 29-32.
 
[13]  Mata, J. A., Martínez-Cánovas, J., Quesada, E.; Béjar, V., 2 002. A detailed phenotypic characterisation of the type strains of Halomonas species. Syst. Appl. Microbiol. 25 (3), 360-375.
 
[14]  Ola, I. O., Akintokun, A. K., Akpan, I., Omomowo, I. O, Areo, V. O. 2010. Aerobic decolourization of two reactive azo dyes under varying carbon and nitrogen source by Bacillu cereus. African Journal of Biotechnology. 9 (5), 672-677.
 
[15]  Pahlaviani K, Massiha A, Issazadeh K(2011) An Innovative Approach to Biodegradation of Textile Azo Dyes by native bacterial strains in Iran. International Conference on Biotechnology and Environment Management. IPCBEE vol. 18
 
[16]  Pasti-Grigsby, M., Paszczynski, S., Goszczynski, D. L., Crawford, and Crawford R. L., 1993. Biodegradation of novel azo dyes. Applied Biotechnology for site remediation. Lewis Publishers, Boca Raton, Fla., p. 384-390.
 
[17]  Pasti-Grigsby, M.B., Burke, N.S., Goszczynski, S. and Crawford, L. 1996. Transformation of azo dye isomers by Streptomyces chromofuscus A11. Appl Environ Microbiol 62, 1814-1817.
 
[18]  Smibert, R. M., Krieg, N. R., 1994. Phenotypic characterization. in methods for general and molecular bacteriology. American Society for Microbiology, 611-651.
 
[19]  Tripathi, A., Srivastava,. S K. 2012. Biodegradation of orange G by a novel isolated bacterial strain Bacillus megaterium ITBHU01 using response surface method ologyafr j biotech 11 (7), 1768-1781.
 
[20]  Wesenberg, D., Kyriakides, I., and Agathos, S.N. 2003. White-rot fungi and their enzymes for the treatment of industrial dye effluents. Biotechnology Advances, 22, 161-187.
 
[21]  Ventosa, A., Quesada, E., Rodriguez-Valera, F., 1982. Numericaltaxonomy of moderately halophilic Gram-negative rods. J. Gen. Microbiol., 128 (9), 1959-1968.