International Journal of Environmental Bioremediation & Biodegradation
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International Journal of Environmental Bioremediation & Biodegradation. 2014, 2(3), 93-99
DOI: 10.12691/ijebb-2-3-1
Open AccessArticle

Bioremedial Application of Bacillus Megaterium PMS82 in Microbial Degradation of Acid Orange Dye

Maulin P Shah1,

1Industrial Waste Water Research Laboratory Applied & Environmental Microbiology Lab, Enviro Technology Limited (CETP), GIDC, Ankleshwar, Gujarat, India

Pub. Date: May 03, 2014

Cite this paper:
Maulin P Shah. Bioremedial Application of Bacillus Megaterium PMS82 in Microbial Degradation of Acid Orange Dye. International Journal of Environmental Bioremediation & Biodegradation. 2014; 2(3):93-99. doi: 10.12691/ijebb-2-3-1

Abstract

A potential bacterial strain PMS82, capable of degrading an azo dye Acid orange as a sole source of carbon was isolated from common effluent treatment plant of Ankleshwar, India. The 16S rDNA sequence and phenotypic characteristics indicated that an isolated organism as Bacillus megaterium PMS82. This strain exhibited complete decolorization of Acid orange (100 mg/L) within 16 h, while maximally it could decolorize 800 mg/L of dye within 38 h with 73% decolorization under static condition. For color removal, the most suitable pH and temperature were pH 6.0-9.0 and 25-40C respectively. The organism has shown more than 70% decolorization activity against five structurally different azo dyes within 38 h and tolerates high salt concentration up to 2% (w/v) respectively with more than 90% decolorization. UV-Visible absorption spectra before and after decolorization suggested that decolorization was due to biodegradation and was further confirmed by FT-IR spectroscopy. Overall results indicating the effectiveness of the strain PMS82 explored for the treatment of common effluent treatment plants containing various azo dyes. To our knowledge, this could be the first report on biodegradation of Acid oragne by Bacillus megaterium PMS82.

Keywords:
biodegradation decolorization bacillus megaterium acid orange textile azo dye

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References:

[1]  McMullan G, Meehan C, Conneely A, Kirby N, Robinson T, Nigam P, Banat IM, Marchant R, Smyth WF: Microbial decolourization and degradation of textile dyes. Appl Microbiol Biotechnol 2001, 81-87.
 
[2]  Kirk RE, Othmer DF: In Dyes, environmental chemistry in: Kirk-Othmer encyclopaedia of chemical technology. 4th edition. Edited by Howe-Grant M. New York: Wiley; 1993: 753-773.
 
[3]  Bell J, Chris A, Buckley A: Treatment of a textile dye in the anaerobic baffled reactor. Water SA 2003, 29: 29-134.
 
[4]  Martins LO, Soares CM, PereiraMM, TeixeraM, Costa T, Jones GH, Henriques AO: Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat. J Biol Chem 2002, 277: 18849-18859.
 
[5]  O’Neill C, Lopez A, Esteves S, Hawkes FR, Hawkes DL, Wilcox S: Azo-dye degradation in an anaerobic-aerobic treatment system operating on simulated textile effluent. Appl Microbiol Biotechnol 2000, 53: 249-254.
 
[6]  Nilsson R, Nordlinder R, Wass U: Asthma, rhinitis, and dermatitis in workers exposed to reactive dyes. Br J Ind Med 1993, 50: 65-70.
 
[7]  Netpradit S, Thiravetyan P, Towprayoon S: Adsorption of three azo reactive dyes by metal hydroxide sludge: effect of temperature, pH, and electrolytes. J Colloid Interface Sci 2004, 270: 255-261.
 
[8]  Robinson T, Chandran B, Nigam P: Studies on the production of enzymes by white-rot fungi for the decolorization of textile dyes. Enzyme Microb Technol 2001, 29: 575-579.
 
[9]  McKay G, Porter JF, Prasad GR: The removal of dye colors from aqueous solutions by adsorption on low cost materials. Water Air Soil Pollut 1999, 114: 423-438.
 
[10]  Ehrampoush MH, Ghanizadeh G, Ghaneian MT: Equilibrium and kinetics study of reactive red 123 dye removal from aqueous solution by adsorption on eggshell. Iran J Environ Health Sci Eng 2011, 8: 101-108.
 
[11]  Lin SH, Peng FC: Continuous treatment of textile waste-water by combined coagulation, electrochemical oxidation and activated sludge. Water Res 1996, 3: 587-592.
 
[12]  Wesenberg D, Kyriakides I, Agathos SN: White-rot fungi and their enzymes for the treatment of industrial dye effluents. Biotechnol Adv 2003, 22: 161-187.
 
[13]  Banat IM, Nigam P, Singh D, Marchant R: Microbial decolorization of textile-dye-containing effluents: a rev. Bioresour Technol 1996, 58: 217-227.
 
[14]  Moreira MT, Mielgo I, Feijoo G, Lema JM: Evaluation of different fungal strains in the decolourisation of synthetic dyes. Biotechnol Lett 2000, 22: 1499-503.
 
[15]  Stolz A: Basic and applied aspects in the microbial degradation of azo dyes. Appl Microbiol Biotechnol 2001, 56 :69-80.
 
[16]  Claus H, Faber G, König H: Redox-mediated decolorization of synthetic dyes by fungal laccases. Appl Microbiol Biotechnol 2002, 59: 672-678.
 
[17]  Couto SR, Sanromán MA, Gübitz GM: Influence of redox mediators and metal ions on synthetic acid dye decolourization by crude laccase from Trametes hirsuta. Chemosphere 2005, 58: 417-422.
 
[18]  Zille A, Tzanov T, Gübitz GM, Cavaco-Paulo A: Immobilized laccase for decolorization of reactive black 5 dyeing effluent. Biotechnol Lett 2003, 25: 1473-1477.
 
[19]  Cripps C, Bumpus JA, Aust ST: Biodegradation of azo and heterocyclic dyes by Phanerochaete chrysosporium. Appl Environ Microbiol 1990, 56: 1114-1118.
 
[20]  Khalaf MA: Biosorption of reactive dye from textile wastewater by nonviable biomass of Aspergillus niger and Spirogyra sp. Bioresour Technol 2008, 99: 6631-6634.
 
[21]  Tatarko M, Bumpus JA: Biodegradation of Congo Red by Phanerochaete chrysosporium. Water Res 1998, 32: 1713-1717.
 
[22]  Willmott N, Guthrie J, Nelson G: The biotechnology approach to color removal from textile effluent. J Soc Dyer Colour 1998, 114: 38-41.
 
[23]  C. Brilon, W. Beckmann, M. Hellwig, and H. J. Knackmuss, “Enrichment and 451 isolation of naphthalene sulfonic acid utilizing Pseudomonads,” Applied and 452 Environmental Microbiology, vol. 42, no. 1, pp. 39-43, 1981.
 
[24]  K. L. Shuttleworth, C .E. Cerniglia, Practical methods for the isolation of polycyclic aromatic hydrocarbon (PAH) degrading microorganisms and biodegradation intermediates, in: J.Hurst (Ed.), Manual of Environmental Microbiology, ASM, Washington, 1996, pp. 766-775.
 
[25]  B. Zhao, S.J.He, Microbiology Exoeriment, 1st ed., Science Press, Beijing, 2002.
 
[26]  G.P.Fergus, G.Michael, T.J.Carole, A.Numerical Classification of the genus Bacillus, J. Gen. Microviol. 134 (1988) 1847-1882.
 
[27]  S.E.Cowan, Manual for the identification of medical Bacteria, 2nd ed., Cambridge University Press, Cambridge, 1974.
 
[28]  A. S. Rosado, G. F. Durate, L. Seldin, J.D. Van Elsas, Genetic diversity of Nifh gene sequence in paenibacillus azotofixans strain and soil samples analyzed by denaturing gradient gel electrophoresis of PCR-amplified gene fragments, Appl. Environ. Microb. 64 (1998) 2770-2779.
 
[29]  M. L. Nievas, M. G. Commendatore, N. L. Oliversa, J. L. Esteves, V. Bucala, Biodegradation of bilge waste from Patagonia with an indigenous microbial community, Bioresour. Technol. 97 (2006). 2280-2289.
 
[30]  A. Khalid, M. Arshad, and D. E. Crowley, “Accelerated decolorization of structurally 471 different azo dyes by newly isolated bacterial strains,” Applied Microbiology and 472 Biotechnology, vol. 78, no. 2, pp. 361-369, 2008.
 
[31]  S. R. Dave and R. H. Dave, “Isolation and characterization of Bacillus thuringiensis 474 for Acid red 119 dye decolorization,” Bioresource Technology, vol. 100, no. 1, pp. 475 249-253, 2009.
 
[32]  D. Cetin and G. Donmez, “Decolorization of reactive dyes by mixed cultures isolated 493 from textile effluent under anaerobic condition,” Enzyme and Microbial technology, 494 vol. 38, pp. 926-930, 2006.
 
[33]  D. C. Kalyani, A. A. Telke, R. Dhanve, and J. P. Jadhav, “Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. 444 SUK1,” Journal of Hazardous Materials, vol. 163, pp. 735-742, 2009.
 
[34]  C. I. Pearce, J. R. Lioyd, and J. T. Gutherie, “The removal of color from textile wastewater using whole bacterial cells: a review,” Dyes and Pigments, vol. 58, no. 3, 491 pp. 179-186, 2003.
 
[35]  D. Cetin and G. Donmez, “Decolorization of reactive dyes by mixed cultures isolated from textile effluent under anaerobic condition,” Enzyme and Microbial technology, 494 vol. 38, pp. 926-930, 2006.
 
[36]  J. Chan and T. Kuo, “Kinetics of bacterial decolorization of azo dye with Escherichia coli NO3,” Bioresource Technology, vol. 75, no. 2, pp. 107-111, 2000.
 
[37]  D. P. Tamboli, M. B. Kurade, T. R. Waghmode, S. M. Joshi, and S. P. Govindwar, “Exploring the ability of Sphingobacterium sp. ATM to degrade textile dye Direct 447 Blue GLL, mixture of dyes and textile effluent and production of 448 polyhydroxyhexadecanoic acid using waste biomass generated after dye degradation,” 449 Journal of Hazardous Materials, vol. 182, pp. 169-176, 2010.
 
[38]  L. A. De Baere, M. Devocht, P. V. Assche, and W. Verstraete, “Influence of high 498 NaCl and NH4Cl salt levels on methanogenic associations,” Water Research, vol. 18, 499 pp. 543-548, 1984. 500
 
[39]  T. Panswad and C. Anan, “Specific oxygen, ammonia and nitrate uptake rates of a biological nutrient removal process treating elevated salinity wastewater,” 502 Bioresource Technology, vol. 70, pp. 237-243, 1999.
 
[40]  P. S. Patil, U. U. Shedbalkar, D. C. Kalyani, and J. P. Jadhav, “Biodegradation of Reactive Blue 59 by isolated bacterial consortium PMB11,” Journal of Industrial 505 Microbiology and Biotechnology, vol. 35, pp. 1181-1190, 2008.
 
[41]  J. Felsenstein, “Confidence limits on phylogenies: An approach using the bootstrap,” vol. 39, pp. 783-791, 1985. 461
 
[42]  N. Takezaki, A. Rzhetsky and M. Nei, “Phylogenetic test of the molecular clock and linearized trees,” Molecular Biology and Evolution, vol. 12, pp. 823-833, 2004.
 
[43]  K. Tamura, M. Nei and S. Kumar, “Prospects for inferring very large phylogenies by using the neighbor-joining method,” Proceedings of the National Academy of Sciences (USA), vol. 101, pp. 11030-11035, 2004.
 
[44]  K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar, “MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary 468 Distance, and Maximum Parsimony Methods,” Molecular Biology and Evolution, vol. 469 28, no. 10, pp. 2731-2739, 2011.
 
[45]  S. Asad, M.A. Amoozegar, A.A. Pourbabaee, M.N. Sarbolouki, and S.M.M. 430 Dastgheib, “Decolorization of textile azo dyes by newly isolated halophilic and 431 halotolerant bacteria,” Bioresource Technology, vol. 98, no. 11, pp. 2082-2088, 2007.